© 2008 Alexis Corporation
ALX-804-515 Revised 30-Aug-06
Monoclonal Antibody to Adiponectin (rat) (RADI 06)
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SYNONYMS anti-ACRP30 (rat) MAb (RADI 06)
anti-Adipocyte Complement Related Protein of 30kDa (rat) MAb (RADI 06)
anti-AdipoQ (rat) MAb (RADI 06)
anti-apM1 (rat) MAb (RADI 06)
anti-GBP28 (rat) MAb (RADI 06)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Adiponectin [ACRP30] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-515-C050   50 µg 195.00 USD Add To Cart
ALX-804-515-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE: RADI 06
ISOTYPE: Mouse IgG2
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant rat adiponectin.
SPECIFICITY: Recognizes both natural and recombinant rat adiponectin.
APPLICATION: ELISA (direct and indirect: 1:5’000-1:10’000).
Western Blot (1:2’000-10’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
BACKGROUND/TECHNICAL INFORMATION
Western Blot Protocol:

1: SDS-PAGE Sample buffer
62.5mM Tris-HCl (pH 6.8), 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.1% (w/v) bromophenol blue.

2: Serum preparation
A. 1µl rat serum and 9µl sample buffer after boiling for 5 min.
B. Recombinant rat adiponectin protein: 100ng/µl and 9µl sample buffer after boiling for 5 min.

3: Electrophoresis

4: Transfer
A. Nitrocellulose membrane
B. Constant 30V for 16 hours.

5: Western blotting
A. Blocking: 0.5% skim milk in TBST
B. Secondary IgG: anti-mouse IgG whole molecular (1:5’000)
C. ECL reaction
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
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