| MW: |
~35-40kDa |
| SOURCE/HOST: |
Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-262) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| CONCENTRATION: |
0.1mg/ml after reconstitution. |
| PURITY: |
≥95% (SDS-PAGE) |
| FORMULATION: |
Lyophilized. Contains PBS. |
| ENDOTOXIN CONTENT: |
<0.1EU/µg purified protein (LAL test; Bio Whittaker). |
| RECONSTITUTION: |
Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
| BIOLOGICAL ACTIVITY: |
Binds to mouse and human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86). |
| SHIPPING: |
SHIPPED ON BLUE ICE |
| LONG TERM STORAGE: |
-20°C |
| USE/STABILITY: |
Stable for at least 6 months after receipt when stored at -20°C. |
| HANDLING: |
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles. |
