ALEXIS Biochemicals: TNF-α, Soluble (mouse) (recombinant)

ALX-522-009

Revised 22-Aug-07

TNF-α, Soluble (mouse) (recombinant)
SYNONYMS	Tumor Necrosis Factor-α, Soluble (mouse) (recombinant) TNFSF 2, Soluble (mouse) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	TNF-alpha

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-009-C050		50 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
MW:	~20kDa (SDS-PAGE).
SOURCE/HOST:	Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY:	Exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells).
SPECIFIC ACTIVITY:	ED₅₀=0.02ng/ml (WEHI 164 cells)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P06804: TNF-α (mouse) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994) Abstract
Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994) Abstract
The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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