ALEXIS Biochemicals: Netrin-4 (human) Detection Set [For ELISA Application]

APO-54N-033

Revised 07-Apr-08

Netrin-4 (human) Detection Set [For ELISA Application]
SYNONYMS	β-Netrin (human) Detection Set [For ELISA Application] Hepar-derived Netrin-like Protein (human) Detection Set [For ELISA Application]
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Netrins & Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-033-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	50pg/ml (range 0 to 5ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human netrin-4 from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

The Netrins (whose name means “one who guides” in Sanskrit) constitutes a family of laminin-related secreted protein. In mice, human and rat, Netrin-1, Netrin-3/NTL2, Netrin-4/β and G-Netrins have been cloned [1]. The Netrins are involved in axonal guidance, are required for regeneration and maintenance of the central nervous system and have been implicated in the development of mammary gland, lung, pancreas, and blood vessel [1, 2]. Netrin-4 (also called β-Netrin) is a novel Netrin more closely related to laminin β chain that is broadly expressed in both neural and non-neural tissues [3]. It acts during the development and controls the migration of neurons [4]. The role of Netrin-4 in guiding neurones seems to be mediated by known Netrin-1 receptors: DCC and UNC5H1 [5]. Netrin-4 gene (NTN4) is transcribed in a variety of cells including epithelial cells and vascular endothelial cells [4]. Recent studies suggest a role of Netrin-1 and 4 in the branching morphogenesis in the lung [2]. Netrin-4 promotes angiogenesis by stimulating proliferation and migration of microvascular endothelial cells to the same extent that vascular endothelial growth factor (VEGF) [6]. Kidney also express Netrin-4 whose level is down regulated upon ischemia-reperfusion injury [7]. Netrin-4 has been reported to be a novel prognostic marker in breast cancer [8].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9HB63: Netrin-4 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Netrins: beyond the brain: V. Cirulli & M. Yebra; Nat. Rev. Mol. Cell Biol. 8, 296 (2007) Abstract
[2] Novel role for Netrins in regulating epithelial behavior during lung branching morphogenesis: Y. Liu, et al.; Curr. Biol. 14, 897 (2004) Abstract
[3] The Netrin family of guidance factors: emphasis on Netrin-1 signalling: M.J. Barallorbre, et al.; Brain Res. Brain Res. Rev. 49, 22 (2005) Abstract
[4] A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization: M. Koch, et al.; J. Cell Biol. 151, 221 (2000) Abstract
[5] Characterization of the receptors for axon guidance factor netrin-4 and identification of the binding domains: S. Qin, et al.; Mol. Cell. Neurosci. 34, 243 (2007) Abstract
[6] Netrins promote developmental and therapeutic angiogenesis: B.D. Wilson, et al.; Science 313, 640 (2006) Abstract
[7] Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney: W. Wang, et al.; Am. J. Physiol. Renal Physiol. 294, F739 (2008) Abstract
[8] Identification of NTN4, TRA1, and STC2 as prognostic markers in breast cancer in a screen for signal sequence encoding proteins: S. Esseghir, et al.; Clin. Cancer Res. 13, 3164 (2007) Abstract

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