© 2008 Alexis Corporation
ALX-581-012 Revised 26-Apr-07
LPS from E. coli, Serotype O111:B4 (TLRgrade™) (liquid)
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SYNONYMS Lipopolysaccharide from E. coli, Serotype O111:B4 S-form (TLRgrade™) (liquid)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-012-L002   2 ml 155.00 USD Add To Cart
Product Specification
RTECS: OJ0895400
SOURCE/HOST: Smooth (S)-form LPS isolated and purified from E. coli O111:B4 by a modification of the phenol water extraction and PCP extraction method [1,2], converted to the uniform sodium salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: Absence of detectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Specific activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at +4°C.
HANDLING: Use must be restricted to qualified personnel. Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream. Keep sterile.
HAZARD: HARMFUL. HIGHLY PYROGENIC.
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Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydrate Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
[4] R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006) Abstract
 
 
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