ALEXIS Biochemicals: EGR-2 Protein (human) (recombinant)

ALX-201-223

Revised 04-Aug-05

EGR-2 Protein (human) (recombinant)
SYNONYMS	Early Growth Response Protein 2 (human) (recombinant) Krox-20 Protein (human) (recombinant) pAT591 (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Egr Protein Family / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-223-1		1 Vial	390.00 USD

Product Specification

SOURCE/HOST:	Produced in Sf9 cells.
QUANTITY:	150µl. Sufficient for >10 Western blots or >70 gel shift assays under standard conditions.
FORMULATION:	Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
BIOLOGICAL ACTIVITY:	Interacts with NFκB protein p65.
APPLICATION:	EMSA promoter characterization, in vitro transcription assay, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as positive control).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

The recombinant EGR-2 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-2 binding promoter elements. b) Detection of EGR-2 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-2. d) Screening for EGR-2 activity in tumor cells and lines. e) Drug screening: Effect on EGR-2 generation and DNA binding.

Product Specific Literature References

Coordinate expression and distinct DNA-binding characteristics of the four EGR-zinc finger proteins in Jurkat T lymphocytes: C. Skerka, et al.; Immunobiology 198, 179 (1997) Abstract
Egr-2 and Egr-3 are negative regulators of T cell activation: M. Safford, et al.; Nat. Immunol. 6, 472 (2005) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either ³²P-ATP or ³³P-ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of ³³P-ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.
Swiss-Prot link P11161: EGR-2 (human)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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