© 2008 Alexis Corporation
ALX-445-004 Revised 08-Oct-08
Phorbol 12-myristate 13-acetate
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SYNONYMS PMA
12-O-Tetradecanoylphorbol 13-acetate
TPA
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Phorbols / Phorbol Esters
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-445-004-M001   1 mg 18.00 USD Add To Cart
ALX-445-004-M005   5 mg 60.00 USD Add To Cart
ALX-445-004-M010   10 mg 110.00 USD Add To Cart
ALX-445-004-M025   25 mg 210.00 USD Add To Cart
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Product Specification
FORMULA: C36H56O8
MW: 616.8
CAS NUMBER: 16561-29-8
MERCK INDEX: 14: 7332
RTECS: QH4377000
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, methanol, 100% ethanol, acetone, ether or dimethylformamide; almost insoluble in aqueous buffers.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Dissolve in an organic solvent prior to dilution into aqueous buffers. Add calculated volume of organic solvent to vial to prepare concentrated stock solution. Intermediate dilutions into the same organic solvent are recommended prior to final dilution into aqueous buffer.
HANDLING: Protect from light.
HAZARD: MAY BE CARCINOGENIC. HARMFUL. MAY BE MUTAGENIC.

Product Description
Most commonly used phorbol ester. Binds to and activates protein kinase C (PKC). Causes an extremely wide range of effects in cells and tissues. Very potent mouse skin tumor promoter. Inhibits apoptosis induced by Fas, but induces apoptosis in HL-60 promyelocytic leukemia cells.
Product Specific Literature References
Autoxidation of phorbol esters under normal storage conditions: R. Schmidt & E. Hecker; Cancer Res. 35, 1375 (1975) Abstract
Phorbol esters as signal transducers and tumor promoters: M. Castagna; Biol. Cell 59, 3 (1987), Review Abstract
P.M. Blumberg; CRC Crit. Rev. Toxicol 8, 153 (1988)
Differential irreversible insertion of protein kinase C into phospholipid vesicles by phorbol esters and related activators: M.G. Kazanietz, et al.; J. Biol. Chem. 267, 20878 (1992) Abstract
Phorbol esters induce nitric oxide synthase activity in rat hepatocytes. Antagonism with the induction elicited by lipopolysaccharide: S. Hortelano, et al.; J. Biol. Chem. 267, 24937 (1992) Abstract; Full Text
Phorbol ester induces apoptosis in HL-60 promyelocytic leukemia cells but not in HL-60 PET mutant: D.E. Macfarlane & P.S. O'Donnel; Leukemia 7, 1846 (1993) Abstract
Effect of phorbol 12-myristate 13-acetate on Ca2+-ATPase activity in rat liver nuclei: K. Oishi & M. Yamaguchi; J. Cell. Biochem. 55, 168 (1994) Abstract
Role for ceramide as an endogenous mediator of Fas-induced cytotoxicity: C.G. Tepper, et al.; PNAS 92, 8443 (1995) Abstract
Evidence for different mechanisms of growth inhibition of T-cell lymphoma by phorbol esters and concanavalin A: S. Desrivieres, et al.; J. Biol. Chem. 272, 2470 (1997) Abstract
Phorbol esters and synthetic diacylglycerols inhibit gamma-aminobutyric acid uptake in human spermatozoa: an effect mediated by protein kinase C?: A. Aanesen, et al.; Mol. Hum. Reprod. 4, 339 (1998) Abstract
Gene expression of TPA induced differentiation in HL-60 cells by DNA microarray analysis: X. Zheng, et al.; Nucleic Acids Res. 30, 4489 (2002) Abstract; Full Text
Phorbol esters and neurotransmitter release: more than just protein kinase C?: E.M. Silinsky and T.J. Searl; Br. J. Pharmacol. 138, 1191 (2003), Review Abstract
Characterization of phorbol esters activity on individual mammalian protein kinase C isoforms, using the yeast phenotypic assay: L. Saraiva, et al.; Eur. J. Pharmacol. 491, 101 (2004) Abstract
Further Categories Containing This Product:
PKC Activators • Natural Products - Apoptosis Inducers & Inhibitors
 
 
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