ALEXIS Biochemicals: ZnAF-2 . tetrahydrochloride

ALX-620-072

Revised 22-Apr-08

ZnAF-2 . tetrahydrochloride
SYNONYMS	6-[N-[N',N'-bis(2-Pyridinylmethyl)-2-aminoethyl]amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one . 4 HCl
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Oxidative Stress Markers & Reagents/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-072-M001		1 mg	380.00 USD

Product Specification

FORMULA:	C₃₄H₂₈N₄O₅ . 4HCl
MW:	572.6 .145.8
CONCENTRATION:	~5mM
PURITY:	≥98% (HPLC)
FORMULATION:	Liquid. In 0.28ml DMSO.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Prepare 500-5'000-fold dilution (~1-10µM) in 100mM HEPES, pH 7.4) immediately before use. Do not store diluted solutions. BSA and phenol red may affect the fluorescence and must be avoided.
HANDLING:	Keep under inert gas. Protect from light. After opening, prepare aliquots and store at +4°C.

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Product Description

Non cell permeable fluorescent reagent (Ex(max): 492nm; Em(max): 514nm) for the detection of low concentration of zinc ion due to its strong affinity to zinc ion (dissociation constant: 2.7nM). The sample zinc ion can be specifically detected. Low background fluorescense supersensitizes the visualization for in vivo sample zinc ion. Also available as cell permeable derivative (Prod. No. ALX-620-076). ZnAF-2 conjugates TPEN (Prod. No. ALX-450-011) affinities with fluorescein and enhances the specificity for Zn²⁺.

Product Specific Literature References

Highly Zinc-Selective Fluorescent Sensor Molecules Suitable for Biological Applications: T. Hirano, et al.; JACS 122, 12399 (2000) Full Text
Mossy fiber Zn2+ spillover modulates heterosynaptic N-methyl-D-aspartate receptor activity in hippocampal CA3 circuits: S. Ueno, et al.; J. Cell Biol. 158, 215 (2002) Abstract; Full Text
Improvement and biological applications of fluorescent probes for zinc, ZnAFs: T. Hirano, et al.; JACS 124, 6555 (2002) Abstract

General Information

Zinc (Zn) is the second most abundant transition metal in the body ant it is essential as catalytic, structural and regulatory ion. Zinc ions are involved in homeostasis, immune responses, oxidative stress, apoptosis and aging. Zinc has been proposed to function as a conventional neurotransmitter for the presynaptic neuron and as a transmembrane signal to traverse the postsynaptic neuron. Aberrant zinc metabolism is associated with many neurological diseases including Alzheimer’s disease, Parkinson’s disease and epilepsy. The most suitable technique for in vivo monitoring of zinc has been proven to be fluroescent imaging.

General Literature References

Fluorescent detection of zinc in biological systems: recent development on the design of chemosensors and biosensors : P. Jiang & Z. Guo; Coord. Chem. Rev. 248, 205 (2004) Full Text
Zinc: a multipurpose trace element: M. Stefanidou, et al.; Arch. Toxicol. 80, 1 (2006) Abstract
Sticky fingers: zinc-fingers as protein-recognition motifs: R. Gamsjaeger, et al.; TIBS 32, 63 (2007) Abstract
Mechanism and regulation of cellular zinc transport: I. Sekler, et al.; Mol. Med. 13, 337 (2007) Abstract; Full Text