ALEXIS Biochemicals: PTX3 (human) Detection Set [For ELISA Application]

ALX-850-299

Revised 18-Aug-08

PTX3 (human) Detection Set [For ELISA Application]
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Pentraxin3 [PTX3]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-299-KI01		1 Set	995.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	Detection limit: 75pg/ml.
KIT/SET CONTAINS:	Contains - 1 vial (10µg) PTX3 (human) (rec.) (Prod. No. ALX-201-124) - 1 vial (50µg) PAb to PTX3 (Biotin) [Detection Antibody] (Prod. No. ALX-210-365B) - 1 vial (100µg) MAb to PTX3 (human) (MNB4) [Capture Antibody] (Prod. No. ALX-804-464)
APPLICATION:	For the quantitative determination of PTX3 in human and bovine (weak) cell culture media, EDTA-plasma or serum (weak) samples.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Once thawed store biotinylated antibody at 4°C and do not re-freeze.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNFalpha, LPS, and cyclic stretch: C.C. dos Santos, et al.; Physiol. Genomics 19, 331 (2004) Abstract; Full Text
TNFalpha-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK: B. Han, et al.; J. Immunol. 175, 8303 (2005) Abstract; Full Text
The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells: P. Baruah, et al.; J. Leukoc. Biol. 80, 87 (2006) Abstract; Full Text
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract
Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris: K. Inoue, et al.; Arterioscler. Thromb. Vasc. Biol. 27, 161 (2007) Abstract; Full Text
Calorie restriction modulates inactivity-induced changes in the inflammatory markers C-reactive protein and pentraxin-3: A. Bosutti, et al.; J. Clin. Endocrinol. Metab. 93, 3226 (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Sandwich ELISA for PTX3

- 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 min. at 405nm in an automatic ELISA reader.

Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.

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