ALEXIS Biochemicals: Annexin V (human) (recombinant) (FITC)

ALX-209-256

Revised 18-May-06

Annexin V (human) (recombinant) (FITC)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-256-T100		100 tests	173.00 USD
ALX-209-256-3100		3x100 tests	366.00 USD

Product Specification

MW:	~52kDa (SDS-PAGE).
QUANTITY:	100 tests/0.5ml; 3x100 tests/3x0.5ml.
PURITY:	≥90%
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least one year after receipt when stored at +4°C.
HANDLING:	Do not freeze. Protect from light.

Product Images

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Product Description

FITC-labelled recombinant human annexin V fusion protein (produced in E. coli) shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm filter).
- Adjust cells to a density between 200'000 and 500'000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-FITC. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)

MANUFACTURER

Manufactured by Apotech Corporation.

Related Products

ALX-209-258	Annexin V (human) (recombinant) (ATTO 488)
ALX-209-251	Annexin V (human) (recombinant) (R-PE)
ALX-209-250	Annexin V (human) (recombinant) (FITC)
ALX-209-252	Annexin V (human) (recombinant) (APC)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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