ALEXIS Biochemicals: NAD Kinase (NT truncated) (active) (human) (recombinant) (His)

ALX-201-236

Revised 17-Aug-06

NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
SYNONYMS	NADK (NT truncated) (active) (human) (recombinant) (His) Poly(P)/ATP NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	NAD+ Metabolism / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-236-C050		50 µg	260.00 USD

Product Specification

EC:	2.7.1.23
SOURCE/HOST:	Produced in E. coli. Human NADK (aa 64-446) is fused at the N-terminus to a His-tag.
QUANTITY:	Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Free of any nucleotide converting side-activities.
FORMULATION:	Liquid. Ammonium sulphate suspension.
RECONSTITUTION:	Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SPECIFIC ACTIVITY:	≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 3 months when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract

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