ALEXIS Biochemicals: Monoclonal Antibody to RIP3 (human) (Rippy-3)

ALX-804-155

Revised 27-Feb-07

Monoclonal Antibody to RIP3 (human) (Rippy-3)
SYNONYMS	anti-Receptor-interacting Protein 3 (human) MAb (Rippy-3) anti-Receptor-interacting Serine/Threonine Protein Kinase 3 (human) MAb (Rippy-3) anti-RIP-like Protein Kinase 3 (human) MAb (Rippy-3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	RIP Kinases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-155-C100		100 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Rippy-3
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human RIP3 (receptor-interacting protein 3).
SPECIFICITY:	Recognizes human RIP3.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles.
POSITIVE CONTROL:	For Positive Control (Western blot) see Prod. No. ALX-840-608.

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General Information

Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These recent data suggest that RIP4 is involved in NF-κB and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF-κB-dependent pro-survival or pro-inflammatory signals [4].

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

[1] Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999) Abstract
[2] RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999) Abstract; Full Text
[3] Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999) Abstract; Full Text
[4] RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002) Abstract; Full Text