© 2008 Alexis Corporation
ALX-300-140
Revised 08-Jul-08
1,2-bis(Heptanoylthio)glycerophosphocholine
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Lipid Signalling Pathways Other Products
Ordering Information
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ALX-300-140-M005
5 mg
215.00 USD
Product Specification
FORMULA:
C
22
H
44
NO
6
PS
2
MW:
513.7
CAS NUMBER:
89019-63-6
PURITY:
≥95%
FORMULATION:
Liquid. Solution in ethanol.
SOLUBILITY:
50 mg/ml soluble in DMSO or dimethyl formamide; sparingly soluble in PBS, pH 7.2 (62μg/ml).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.
Product Description
Substrate for the general assay of phospholipases, except Ca
2+
-dependent cytosolic phospholipase A
2
(cPLA
2
). PLA
2
hydrolyzes the
sn
-2 position of the phospholipid thiol groups. The released thiol can be estimated colorimetrically using Ellman's reagent (DTNB, Prod. No.
ALX-400-034
). For an assay kit for secretory PLA
2
(sPLA
2
) based on this substrate see Prod. No.
ALX-850-007
. For a substrate for cPLA
2
see Arachidonoyl Thio-PC (Prod. No.
ALX-300-141
).
Product Specific Literature References
Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: development of a spectrophotometric assay suitable for a microtiterplate reader:
L.J. Reynolds, et al.; Anal. Biochem.
204
, 190 (1992)
Abstract
Thio-based phospholipase assay:
L. Yu & E.A. Dennis; Meth. Enzymol.
197
, 65 (1991)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice. Avoid dissolving 1,2-bis(Heptanoylthio)glycerophosphocholine in basic solutions, since base treatment promotes hydrolysis of the thioester. Triton X-100 will enhance the solubility significantly. The critical micellar concentration of 1,2-bis(Heptanoylthio)glycerophosphocholine 0.17mM in 0.2M Tris-maleate buffer, pH 7.4.
Further Categories Containing This Product:
PLA2 Substrates
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