| EC: |
2.1.1.63 |
| SOURCE/HOST: |
Produced in E. coli. Full length human MGMT is fused at the N-terminus to a GST-tag. |
| PURITY: |
Purified with Glutathiose-Sepharose 4B and thrombin cleavage |
| FORMULATION: |
Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA. |
| SPECIFIC ACTIVITY: |
100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O6-methylguanine from DNA. |
| QUALITY CONTROL: |
The biological activity has been checked in a radioactive standard MGMT assay. |
| SHIPPING: |
SHIPPED ON BLUE ICE |
| LONG TERM STORAGE: |
-20°C |
| HANDLING: |
Do not store at -80°C. |