© 2008 Alexis Corporation
ALX-610-022
Revised 25-Apr-08
2',7'-Dichlorodihydrofluorescein diacetate
SYNONYMS
DCDHF diacetate
2',7'-Dichlorofluorescein diacetate
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
Ordering Information
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ALX-610-022-M050
50 mg
43.00 USD
Product Specification
FORMULA:
C
24
H
16
O
7
Cl
2
MW:
487.3
CAS NUMBER:
4091-99-0
PURITY:
≥95%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
25mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately diluted with PBS, pH 7.2, to the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1mg/ml. We do not recommend storing the aqueous solution for more than one day.
Product Description
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=79,500M
-1
cm
-1
). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.
Product Specific Literature References
Peroxynitrite-mediated oxidation of dihydrorhodamine 123:
N.W. Kooy, et al.; Free Radic. Biol. Med.
16
, 149 (1994)
Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species:
J.P. Crow; Nitric Oxide
1
, 145 (1997)
Abstract
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate:
S.L. Hempel, et al.; Free Radic. Biol. Med.
27
, 146 (1999)
Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123:
H. Ischiropoulos, et al.; Meth. Enzymol.
301
, 367 (1999)
Abstract
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
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