ALEXIS Biochemicals: Osteoprotegerin (human):Fc (human) (recombinant)

ALX-522-007

Revised 30-Jul-07

Osteoprotegerin (human):Fc (human) (recombinant)
SYNONYMS	OPG (human):Fc (human) (recombinant) TNFRSF 11B (human):Fc (human) (recombinant) Osteoclastogenesis Inhibitory Factor (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-007-C050		50 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human OPG (osteoprotegerin) (aa 22-202) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human and mouse RANKL and TRAIL.
BIOLOGICAL ACTIVITY:	Inhibits rhsTRAIL-mediated apoptosis at a concentration range of 5-20µg/ml, as well as rhsRANKL-induced osteoclastogenesis and stimulation of dendritic cells. Concentrations of rhOPG:Fc required to inhibit may vary depending on the cell type studied and on the concentration of rhsTRAIL used to kill the cells.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
A CD40-CD95L fusion protein interferes with CD40L-induced prosurvival signaling and allows membrane CD40L-restricted activation of CD95: C. Assohou-Luty, et al.; J. Mol. Med. 84, 785 (2006) Abstract

General Information

Osteoprotegerin (OPG) is a secreted tumor necrosis factor receptor homologue that inhibits osteoclastogenesis and increases bone density in vivo. OPG contains 4 cysteine-rich repeats and 2 ‘death domains’ (DD). OPG binds to RANKL (TRANCE) and with a similar affinity to TRAIL.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O00300: Osteoprotegrin (human) (precursor) AfCS Signalling Gateway link A002819: Osteoprotegrin (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.