© 2008 Alexis Corporation
ALX-200-422 Revised 07-Nov-07
MMP-9 Proenzyme (human), Monomer
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SYNONYMS Gelatinase B Proenzyme (human), Monomer
Matrix Metalloproteinase 9 Proenzyme (human), Monomer
Collagenase (92kDa Type IV) Proenzyme (human), Monomer
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-422-C005   5 µg 121.00 USD Add To Cart
Product Specification
MW: ~92kDa.
EC: 3.4.24.35
SOURCE/HOST: Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
CONCENTRATION: 100µg/ml 
PURITY: ≥95% (SDS-PAGE, Western blot)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% Brij 35, and 0.05% NaN3.
SPECIFIC ACTIVITY: ≥1’300mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for 1 week when stored at +4°C and for serveral weeks when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Description
MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) for 60-120 min. or 100µg/ml TPCK-trypsin for 30 min. at 37°C. If required trypsin can be inhibited by incubation with 1µl of 1mg/ml aprotinin solution for 10 min. at 25°C.
If working with MMP-9/TIMP-1 complexes, it is recommended to use stromelysin-1 (MMP-3) for activation. Incubation at 37°C for 2 hours at 40:1 (MMP-9:stromelysin-1) will remove only the propeptide to give the active form of the enzyme.

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link P14780: MMP-9 (human) (precursor)
AfCS Signalling Gateway link A001479: MMP-9 (mouse)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
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