ALEXIS Biochemicals: Pertussis Toxin A Protomer

ALX-630-080

Revised 01-Nov-07

Pertussis Toxin A Protomer
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Neurotoxins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-630-080-C010		10 µg	250.00 USD

Product Specification

MW:	28kDa
SOURCE/HOST:	Isolated from Bordetella pertussis.
PURITY DETAIL:	One major band by SDS-PAGE; <1% holotoxin by CHO cell assay.
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with 500µl distilled water to 10µg of protein in 10mM TRIS, pH 8.0, containing 0.1mM Na₂ETDA and 0.03% CHAPS.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HAZARD:	TOXIC.

Product Description

The A protomer (S-1) of pertussis toxin possesses both NAD⁺glycohydrolase and ADP-ribosyltransferase activities, but is unable to penetrate cells in the absence of B oligomer. It is, however, as effective as native pertussis toxin in catalyzing ADP-ribosylation in broken cell preparations.

Product Specific Literature References

Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model: M. Tamura, et al.; Biochemistry 21, 5516 (1982) Abstract
Activation by thiol of the latent NAD glycohydrolase and ADP- ribosyltransferase activities of Bordetella pertussis toxin (islet- activating protein): J. Moss et al.; J. Biol. Chem. 258, 11879 (1983) Abstract; Full Text
ADP-ribosylation of G proteins with pertussis toxin: G.S. Kopf & M.J. Woolkalis; Meth. Enzymol. 195, 257 (1991) Abstract
Pertussis toxin and target eukaryotic cells: binding, entry, and activation: H. R. Kaslow & D. L. Burns; FASEB J. 6, 2684 (1992), (Review) Abstract
A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit: C. Locht and R. Antoine; Biochimie 77, 333 (1995), (Review) Abstract

Further Categories Containing This Product:

Adenylyl Cyclase Activators • ADP Ribosylation Factor [ARF] / Related Products

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