ALEXIS Biochemicals: DNA Ligase I (human) (recombinant)

ALX-201-278

Revised 19-Mar-08

DNA Ligase I (human) (recombinant)
SYNONYMS	DNA Ligase 1 (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Replication Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-278-C010		10 µg	260.00 USD

Product Specification

MW:	~102kDa. Note: The mobility of DNA ligase I in a PAA gel is not corresponding to its MW; it runs at ~125kDa.
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.6mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM HEPES-KOH, pH 7.5, containing 30mM sodium chloride, 1mM EDTA, 1mM DTT and 30% glycerol.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
USE/STABILITY:	Enzyme is freshly produced upon customer request and is stable for ~2 weeks at -80°C. Very prone to degradation even in the absence of proteases. Freeze in liquid nitrogen for long term storage.
HANDLING:	After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.

Product Specific Literature References

The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair: A. Gembka, et al.; Nucleic Acids Res. 35, 2596 (2007) Abstract; Full Text

Related Products

ALX-201-276	DNA Polymerase λ (human) (recombinant)
ALX-201-277	Apurinic/Apyrimidinic Endonuclease (human) (recombinant)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • DNA Repair Other Products

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