ALEXIS Biochemicals: QUIN 2 . tetrapotassium salt

ALX-620-006

Revised 04-Mar-05

QUIN 2 . tetrapotassium salt
SYNONYMS	2-[(2-bis-[Carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis[carboxymethyl]aminoquinoline . 4K
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Chelators / Caged Calcium Chelators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-006-M005		5 mg	35.00 USD

Product Specification

FORMULA:	C₂₆H₂₃N₃O₁₀ . 4K
MW:	537.5 . 156.4
CAS NUMBER:	73630-23-6
MERCK INDEX:	14: 8042
PURITY:	≥95% (contains 11-13% water)
APPEARANCE:	Yellow powder.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Calcium specific chelator and indicator. Upon binding calcium there is a shift in the UV spectrum and an enhancement of fluorescence. QUIN 2 was a member of the first generation of Ca²⁺ chelators introduced by Tsien in 1980. These chelators have backbones similar to that of EGTA (ethylenglycol-bis(β-aminoethyl)-N,N,N',N'-tetraacetic acid) and, like EGTA, they are able to chelate Ca²⁺ with high affinity and specifity. Moreover, these molecules contain aromatic rings that interact electronically and sterically with the chelating backbone. Thus chelation results in changes in the UV-absorbing and fluorescing properties of the molecules. The resulting spectral changes allow these substances to be used as very sensitive probes for Ca²⁺ ions, Ca²⁺ concentrations in single cells of various types can be measured for the first time! TPEN (Prod. No. ALX-450-011) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Product Specific Literature References

Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator: R.Y. Tsien, et al.; J. Cell. Biol. 94, 325 (1982) Abstract
Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas. A new, membrane-permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free Ca2+: P. Arslan, et al.; J. Biol. Chem. 260, 2719 (1985) Abstract; Full Text
Calcium transients in human platelets monitored by aequorin, fura-2 and quin-2: effects of protein kinase C activation and inhibition: P. Erne, et al.; BBRC 145, 66 (1987) Abstract
Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli: I. Maruyama, et al.; FEBS Lett. 220, 89 (1987) Abstract
Effects of interferon on natural killer (NK) cells assessed by fluorescent probes and flow cytometry: K. McGinnes, et al.; J. Immunol. Meth. 107, 129 (1988) Abstract
Measurement and manipulation of cytoplasmic free calcium of ram and boar spermatozoa using quin 2: A.M. Simpson & I.G. White; Cell Calcium 9, 45 (1988) Abstract
Measurement of the cytosolic free calcium ion concentration of individual lymphocytes by microfluorometry using quin 2 or fura-2: H. Komada, et al.; Cell Struct. Funct. 14, 141 (1989) Abstract
Quantitation of bovine sperm cytoplasmic calcium with Quin-2 and Fura- 2: evidence that external calcium does not have direct access to the sperm cytoplasm: S. Vijayaraghavan & D. Hoskins; Cell Calcium 10, 241 (1989) Abstract

Further Categories Containing This Product:

MDR Other Products • Calcium Indicators / Probes • Dyes / Stains / Fluorescent Probes / Fluorescent Labels

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