ALEXIS Biochemicals: MMP-9 Proenzyme (human), Monomer

ALX-200-422

Revised 12-Nov-08

MMP-9 Proenzyme (human), Monomer
SYNONYMS	Gelatinase B Proenzyme (human), Monomer Matrix Metalloproteinase 9 Proenzyme (human), Monomer Collagenase (92kDa Type IV) Proenzyme (human), Monomer
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	MMPs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-422-C005		5 µg	121.00 USD

Product Specification

MW:	~92kDa.
EC:	3.4.24.35
SOURCE/HOST:	Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
CONCENTRATION:	100µg/ml
PURITY:	≥95% (SDS-PAGE, Western blot): no other MMP contaminants are detectable
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl₂, 1µM ZnCl₂, 0.05% Brij 35, and 0.05% NaN₃.
SPECIFIC ACTIVITY:	≥1’400mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for 1 week when stored at +4°C and for serveral weeks when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Description

MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin.

Product Specific Literature References

Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) for 60-120 min. or 100µg/ml TPCK-trypsin for 30 min. at 37°C. If required trypsin can be inhibited by incubation with 1µl of 1mg/ml aprotinin solution for 10 min. at 25°C.
If working with MMP-9/TIMP-1 complexes, it is recommended to use stromelysin-1 (MMP-3) for activation. Incubation at 37°C for 2 hours at 40:1 (MMP-9:stromelysin-1) will remove only the propeptide to give the active form of the enzyme.

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link P14780: MMP-9 (human) (precursor)
AfCS Signalling Gateway link A001479: MMP-9 (mouse)

Further Categories Containing This Product:

Natural Proteins • Enzymes

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