ALEXIS Biochemicals: Convulxin

ALX-350-100

Revised 03-Apr-08

Convulxin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Toxins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-100-C050		50 µg	565.00 USD

Product Specification

MW:	~84kDa.
CAS NUMBER:	37206-04-5
SOURCE/HOST:	Isolated from Crotalus durissus terrificus snake venom.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized.
SOLUBILITY:	Soluble in organic buffer (HEPES).
ACTIVITY:	<50ng/ml (minimal concentration to induce maximum activation of washed human platelets).
APPLICATION:	Studies on platelet receptors.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for 8 hours at +20°C, for 2 days at +4°C or for 1 month when stored at -80°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
HAZARD:	TOXIC.

Product Description

Heterodimeric C-type lectin. Activates mammalian platelets via binding and clustering of p62/GPVI-receptors under physiological conditions.

Product Specific Literature References

Convulxin, a new toxin from the venom of the South American rattlesnake Crotalus durissus terrificus: J. Prado-Franceschi & O.V. Brazil; Toxicon 19, 875 (1981) Abstract
Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor: J. Polgar, et al.; J. Biol. Chem. 272, 13576 (1997) Abstract
Convulxin-induced platelet adhesion and aggregation: involvement of glycoproteins VI and IaIIa: M. Jandrot-Perrus, et al.; Platelets 9, 207 (1998) Abstract
Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases: M. Cicmil, et al.; J. Biol. Chem. 275, 27339 (2000) Abstract
Convulxin binds to native, human glycoprotein Ib alpha: S. Kanaji, et al.; J. Biol. Chem. 278, 39452 (2003) Abstract
Structure of the snake-venom toxin convulxin: T. Batuwangala, et al.; Acta Crystallogr. D. Biol. Crystallogr. 60, 46 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Assay performed in aggregometer:
- Incubate 485µl platelets suspension (5x10⁸ platelets/ml) for 1-2 minutes at 37°C until base line is stable.
- Add 5µl 200mM CaCl₂.
- Add 5µl 200mM MgCl₂.
- Wait until baseline is stable.
- Add 5µl collagen solution (0.5mg/ml) for positive control OR add 5µl convulxin (0.1µg/ml in HEPES).
- Measure aggregation at 37°C.

Further Categories Containing This Product:

Unconjugated Lectins • Platelet Activating Factors [PAF] & Receptors / Related Products

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