ALEXIS Biochemicals: Monoclonal Antibody to Apaf-1 (human) (2E12)

ALX-804-348

Revised 18-Sep-07

Monoclonal Antibody to Apaf-1 (human) (2E12)
SYNONYMS	anti-Apoptosis Protease-activating Factor 1 (human) MAb (2E12)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Apaf-1

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-348-C100		100 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	2E12
ISOTYPE:	Rat IgG2a
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Apaf-1 (apoptosis protease-activating factor 1) N-terminal CARD and CED-4 homologous domains (aa 1-464) .
SPECIFICITY:	Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
APPLICATION:	ELISA Immunocytochemistry Immunohistochemistry (EM on frozen sections; for details see G. Hausmann, et al., below.) Immunoprecipitation Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1: K. Moriishi, et al.; PNAS 96, 9683 (1999) Abstract; Full Text
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol. 149, 623 (2000) Abstract; Full Text
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines: K. Adachi, et al.; Int. J. Oncol. 23, 713 (2003) Abstract
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein: D.H. Cho, et al.; J. Biol. Chem. 279, 39942 (2004) Abstract; Full Text
BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004) Abstract

General Literature References

Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell 94, 727 (1998) Abstract
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS 95, 4386 (1998) Abstract; Full Text
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci. 58, 1688 (2001), (Review) Abstract

Further Categories Containing This Product:

Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies

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