ALEXIS Biochemicals: Polyclonal Antibody to Cardif (human) (AT107)

ALX-210-929

Revised 16-Apr-08

Polyclonal Antibody to Cardif (human) (AT107)
SYNONYMS	anti-CARD Adapter Inducing Interferon-β (human) PAb (AT107) anti-IPS-1 (human) PAb (AT107) anti-Interferon-β Promoter Stimulator Protein 1 (human) PAb (AT107) anti-MAVS (human) PAb (AT107) anti-Mitochondrial Antiviral Signalling Protein (human) PAb (AT107) anti-VISA (human) PAb (AT107) anti-Virus-induced Signalling Adapter (human) PAb (AT107)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antiviral Signalling / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-929-C100		100 µg	370.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Epitope-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY:	Recognizes human cardif.
APPLICATION:	Immunocytochemistry (1:500) Immunoprecipitation (1:100) Western Blot (1:2’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Specific Literature References

Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006) Abstract
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract

General Information

RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract

Further Categories Containing This Product:

Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Polyclonal Antibodies

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