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ALX-200-602 Revised 16-Oct-07
Granzyme B (human)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-602-C010   10 µg 495.00 USD Add To Cart
Product Specification
MW: ~32kDa.
EC: 3.4.21.79
SOURCE/HOST: Isolated from IL-2 activated human lymphocytes [1]. Active enzyme.
CONCENTRATION: ~5.6mg/ml
FORMULATION: Liquid. In 150mM sodium chloride. Contains no preservatives.
BIOLOGICAL ACTIVITY: Using standard cell lines, 10µg is sufficient to kill 1x108 cells [2].
SPECIFIC ACTIVITY: ≥10U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol BAADT per min. (Prod. No. ALX-260-050) [1].
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Do not dilute below 1mg/ml. Dilute solutions should be used within 24 hours.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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Product Specific Literature References
[1] Rapid purification of cationic granule proteases: application to human granzymes: W.L. Hanna, et al.; Protein Purif. Exp. 4, 398 (1993) Abstract
[2] New paradigm for lymphocyte granule-mediated cytotoxicity. Target cells bind and internalize granzyme B, but an endosomolytic agent is necessary for cytosolic delivery and subsequent apoptosis: C.J. Froelich, et al.; J. Biol. Chem. 271, 29073 (1996) Abstract; Full Text
[3] Granzyme B binds to target cells mostly by charge and must be added at the same time as perforin to trigger apoptosis: L. Shi, et al.; J. Immunol. 174, 5456 (2005) Abstract
[4] Granzyme A induces a pro-inflammatory cytokine response and not cell death.: C. J. Froelich, et al.; Immunity Epub ahead of print, (2007)
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P10144: Granzyme B (human) (precursor)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
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