ALEXIS Biochemicals: Annexin V (human) (recombinant) (FITC)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-250-T020		20 tests	106.00 USD
ALX-209-250-T100		100 tests	352.00 USD
ALX-209-250-T300		300 tests	531.00 USD

Product Specification

MW:	~35.8kDa.
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY:	≥98% (SDS-PAGE, HPLC)
FORMULATION:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	After opening, prepare aliquots and store at -20°C.

Product Description

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).

Product Specific Literature References

[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through
0.2µm pore filter).
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC- mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)