ALEXIS Biochemicals: Dihydrorhodamine 123

ALX-610-021

Revised 04-Nov-05

Dihydrorhodamine 123
SYNONYMS	DHR
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Dyes / Stains / Fluorescent Probes / Fluorescent Labels

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-610-021-M005		5 mg	153.00 USD
ALX-610-021-M010		10 mg	272.00 USD

Product Specification

FORMULA:	C₂₁H₁₈N₂O₃
MW:	346.4
CAS NUMBER:	109244-58-8
PURITY:	≥98%
APPEARANCE:	Pink crystalline solid.
SOLUBILITY:	Soluble in organic solvents such as ethanol, DMSO or dimethyl formamide.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for at least 6 months when stored at -20°C.

Product Description

Sensitive indicator of peroxynitrite formation. DHR is oxidized by peroxynitrite to the highly fluorescent product rhodamine. Formation of rhodamine can be monitored by fluorescence spectroscopy (Ex(max): 500nm, Em(max): 536nm), or by absorbance spectroscopy at 500nm (ε=78'800M^-1cm^-1). Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.

Product Specific Literature References

Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994) Abstract
Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen: K. Briviba, et al.; Biochem. J. 319, 13 (1996) Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997) Abstract
Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase: H. Sies, et al.; J. Biol. Chem. 272, 27812 (1997) Abstract; Full Text
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999) Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999) Abstract

Further Categories Containing This Product:

Respiratory Chain Other Products • Peroxynitrite / Scavengers / Detection

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