ALEXIS Biochemicals: (E)-4-Hydroxynonenal-dimethylacetal

ALX-270-375

Revised 04-Feb-05

(E)-4-Hydroxynonenal-dimethylacetal
SYNONYMS	HNE-DA
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Oxidative Stress Markers & Reagents/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-375-1		1 Vial	98.00 USD

Product Specification

FORMULA:	C₁₁H₂₂O₃
MW:	202.3
PURITY:	≥85% (HPLC)
FORMULATION:	Liquid. 6.8mg in 1ml n-hexane. Yields ~5.2mg aldehyde after hydrolysis.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Unopened vial is stable for at least one year after receipt.
HANDLING:	Keep cool and dry.

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Product Description

Derivative from (E)-4-hydroxynonenal (HNE) (Prod. No. ALX-270-245). Can be used to freshly prepare HNE.

Product Specific Literature References

Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes: H. Esterbauer, R.J. Schaur & H. Zollner; Free Radic. Biol. Med. 11, 81 (1991) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Preparation and use of (E)-4-Hydroxynonenal (HNE) from the Acetal (HNE-DA):
- Evaporate the n-hexane under a gentle stream of nitrogen at RT. Continue to work at +4°C.
- Add 1ml of 1mM HCl (+4°C) and stir for about 45 minutes or close the vial and shake for 30 minutes.
- The initially cloudy suspension becomes almost clear as saponification proceeds.
- The resulting aqueous HNE solution has a pH of 3 and contains ~5.2mg of aldehyde.
- This solution, if kept at +4°C, is stable for at least 24 hours.
- If neutralized (see Note) this product is stable for 6 hours.
- We recommend to use the in situ prepared HNE immediately.
Note: HNE decomposes (polymerizes) in basic solution within a few minutes. Never use alkalies to neutralize the pH, but rather appropriately concentrated buffers. During saponification, methanol is generated. If methanol is undesirable, HNE can be extracted in chloroform or dichloromethane. HNE is only sparingly soluble in water.

ALX-210-767	Polyclonal Antibody to (E)-4-Hydroxynonenal
ALX-210-767	Polyclonal Antibody to (E)-4-Hydroxynonenal
ALX-270-245	(E)-4-Hydroxynonenal
ALX-270-406	(E)-4-Hydroxynonenal-d₃
ALX-270-375	(E)-4-Hydroxynonenal-dimethylacetal