ALEXIS Biochemicals: EGR-1 Protein (human) (recombinant)

ALX-201-074

Revised 18-Sep-07

EGR-1 Protein (human) (recombinant)
SYNONYMS	Early Growth Response Protein 1 (human) (recombinant) NGFI-A (human) (recombinant) krox24 (human) (recombinant) TIS-8 (human) (recombinant) zif 268 (human) (recombinant) pat 225 (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Egr Protein Family/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-074-1		1 Vial	365.00 USD

Product Specification

SOURCE/HOST:	Produced in Sf9 cells.
QUANTITY:	50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions [2].
FORMULATION:	Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
SPECIFICITY:	Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
SPECIFIC ACTIVITY:	>0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
APPLICATION:	EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.

Product Specific Literature References

A regulatory element in the human interleukin 2 gene promoter is a binding site for the zinc finger proteins Sp1 and EGR-1: C. Skerka, et al.; J. Biol. Chem. 270, 22500 (1995) Abstract; Full Text
The early growth response protein (EGR-1) regulates interleukin-2 transcription by synergistic interaction with the nuclear factor of activated T cells: E.L. Decker, et al.; J. Biol. Chem. 273, 26923 (1998) Abstract; Full Text
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor: P.F. Zipfel, et al.; Biochim. Biophys. Acta. 1354, 134 (1997) Abstract
Zinc finger transcription factors as molecular targets for nitric oxide-mediated immunosuppression: inhibition of IL-2 gene expression in murine lymphocytes: D. Berendji, et al.; Mol. Med. 5, 721 (1999) Abstract
Fibroblast growth factor-2 induction of platelet-derived growth factor-C chain transcription in vascular smooth muscle cells is ERK-dependent but not JNK-dependent and mediated by Egr-1: V.C. Midgley and L.M. Khachigian; J. Biol. Chem. 279, 40289 (2004) Abstract; Full Text

General Information

Early Growth Response- (EGR-) proteins represent a family of related transcription factors with almost identical DNA- binding zinc finger domains, but with different flanking regions. All four human EGR-proteins, EGR-1 to EGR-4 bind the EGR-consensus sequence GCG T/GGG GCG. However individual EGR-proteins show different binding to related sequences, such as the overlapping EGR-1/Sp1 binding site in the human IL-2 gene promoter. In functional assays the EGR-4 protein shows superior performance for Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E.coli.

BACKGROUND/TECHNICAL INFORMATION

For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either 32P-γ ATP or 33P-γ ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of 33P-γ ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.

Swiss-Prot link P18146: EGR-1 (human)
AfCS Signalling Gateway link A003269: EGR-1 (mouse)

General Literature References

A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization: V.P. Sukhatme, et al.; Cell 53, 37 (1988) Abstract
Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals: X.M. Cao, et al.; Mol. Cell. Biol. 10, 1931 (1990) Abstract
Early growth response protein 1 (Egr-1): prototype of a zinc-finger family of transcription factor: A. Gashler & V.P. Sukhatme; Prog. Nucleic Acid Res. Mol. Biol. 50, 191 (1995) Abstract

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

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