ALEXIS Biochemicals: FURA 2 . pentapotassium salt

ALX-620-004

Revised 21-May-08

FURA 2 . pentapotassium salt
SYNONYMS	1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid . 5K
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Chelators/Caged Calcium Chelators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-004-M001		1 mg	110.00 USD

Product Specification

FORMULA:	C₂₉H₂₂N₃O₁₄ . 5K
MW:	636.5 . 195.5
CAS NUMBER:	113694-64-7
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow to orange solid.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light and moisture.
IDENTITY:	Identity determined by NMR and fluorescence.

Product Description

Ca²⁺ chelator that offers more than 30-fold greater fluorescence intensity than QUIN 2, (Prod. No. ALX-620-006), allowing much smaller amounts to be used and minimizes toxic effects and other artifacts. Shows a strong shift in UV absorption maxima on chelation of Ca²⁺. Ca²⁺ concentration has been shown to be closely correlated to the ratios of fluorescence, measured at ~510nm, when excitated at 340nm and at 380nm. This ratioing method allows estimation of Ca²⁺ concentration independent of the dye concentration. Used in many cellular systems, including cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules.

Product Specific Literature References

Spontaneous and experimentally evoked [Ca2+]i-transients in cardiac myocytes measured by means of a fast Fura-2 technique: S. Bals, et al.; Cell Calcium 11, 385 (1990) Abstract
Measurement of intracellular Ca2+ in cultured arterial smooth muscle cells using Fura-2 and digital imaging microscopy: W.F. Goldman, et al.; Cell Calcium 11, 221 (1990) Abstract
Ryanodine reveals multiple contractile and relaxant mechanisms in vascular smooth muscle: simultaneous measurements of mechanical activity and of cytoplasmic free Ca2+ level with fura-2: T. Hisayama, et al.; Br. J. Pharmacol. 100, 677 (1990) Abstract
Cholinergic agonists increase cell calcium in rat medullary collecting tubules. A fura-2 study: J. Marchetti, et al.; Pfluegers Arch. 416, 561 (1990) Abstract
Estimation of intracellular calcium activity in confluent monolayers of primary cultures of quail medullary bone osteoclasts: S. Arkle, et al.; Exp. Physiol. 79, 975 (1994) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

FURA 2 pentapotassium salt is not cell-permeable. Cells need to be loaded by pressure microinjection or permeabilized prior to use this compound. These approaches are only suggestions as optimal conditions should be determined by individual users.

Further Categories Containing This Product:

MDR Other Products • Calcium Indicators/Probes • Dyes/Stains/Fluorescent Probes/Fluorescent Labels

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