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ALX-210-833 Revised 07-Nov-07
Polyclonal Antibody to MMP-13 (human)
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SYNONYMS anti-Matrix Metalloproteinase 13 (human) PAb
anti-Collagenase-3 (human) PAb
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Antibodies
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-833-R100   100 µl 520.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.5, containing 0.01% thimerosal.
IMMUNOGEN: Recombinant human MMP-13 (matrix metalloproteinase 13).
SPECIFICITY: Recognizes human MMP-13. Does not cross-react with human MMP-2, MMP-9, MMP-14, MMP-15, MMP-16 or MMP-17.
APPLICATION: ELISA (detection; 1:2’000-1:4’000)
Western Blot (ECL-detection; 1:2’500 - 1:8’000)
Note: For capture antibody use MAb to MMP-13 (Prod. No. ALX-804-365).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-834 Revised 04-Dec-04
Polyclonal Antibody to MMP-14 (human)
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SYNONYMS anti-Matrix Metalloproteinase 14 (human) PAb
anti-Membrane-type 1 Matrix Metalloproteinase (human) PAb
anti-MT1-MMP (human) PAb
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-834-R100   100 µl 520.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Purified. Contains 0.01% thimerosal.
IMMUNOGEN: Synthetic peptide derived from hinge region between catalytic domain and hemopexin domain of MMP-14 (matrix metalloproteinase 14).
SPECIFICITY: Recognizes human MMP-14. Does not cross-react with human MMP-15.
APPLICATION: ELISA
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-876 Revised 26-Jun-08
Polyclonal Antibody to MMP-1
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SYNONYMS anti-Matrix Metalloproteinase 1 PAb
anti-Collagenase-1 PAb
anti-Interstitial Collagenase PAb
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Antibodies
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ALX-210-876-C100   100 µg 303.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST: From guinea pig.
CONCENTRATION: 0.5mg/ml
FORMULATION: Liquid. In PBS. Contains no preservatives.
IMMUNOGEN: Matrix metalloproteinase 1 (MMP-1) from rabbit synovial fibroblasts.
SPECIFICITY: Recognizes human, rabbit and marmoset MMP-1. Moderately cross-reacts with human MMP-3.
APPLICATION: ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blot (400-800ng/ml).
Optimal conditions should be determined individually.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Localization of matrix metalloproteinase 9 (92-kilodalton gelatinase/type IV collagenase = gelatinase B) in osteoclasts: implications for bone resorption: Y. Okada, et al.; Lab. Invest. 72, 311 (1995) Abstract
Matrix metalloproteinase 13 (collagenase 3) in human rheumatoid synovium: O. Lindy, et al.; Arthritis Rheum. 40, 1391 (1997) Abstract
The activity of collagenase-1 is required for keratinocyte migration on a type I collagen matrix: B.K. Pilcher, et al.; J. Cell Biol. 137, 1445 (1997) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-877 Revised 21-Dec-06
Polyclonal Antibody to MMP-3
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SYNONYMS anti-Matrix Metalloproteinase 3 PAb
anti-Procollagenase PAb
anti-Proteoglycanase PAb
anti-Stromelysin-1 PAb
anti-Transin-1 PAb
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Antibodies
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ALX-210-877-C100   100 µg 363.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST: From guinea pig.
FORMULATION: Liquid. Purified IgG fraction in PBS. Contains no preservatives.
IMMUNOGEN: MMP-3 (matrix metalloproteinase 3) from rabbit synovial fibroblasts.
SPECIFICITY: Recognizes human, rabbit and marmoset MMP-3. Does not cross-react with MMP-1, MMP-2, MMP-8 or MMP-9.
APPLICATION: Immunoprecipitation (10-30µg/ml)
Western Blot (400-900ng/ml).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-880 Revised 20-May-08
Polyclonal Antibody to VASP (affinity purified)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein (affinity purified)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP / Related Products
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ALX-210-880-C025   25 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
SOURCE/HOST: From rabbit.
CONCENTRATION: 250µg/ml.
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN: Recombinant human VASP.
SPECIFICITY: Recognizes human, mouse and pig VASP. Detects both the 46kDa (Ser157 dephospho) and 50kDa (Ser157 phospho) forms of VASP.
APPLICATION: Immunocytochemistry (formaldehyde fixed cells; 1µg/ml)
Immunoprecipitation (2µg/ml)
Western Blot (0.1µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
POSITIVE CONTROL: Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).
Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
General Information
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-894 Revised 12-Sep-05
Polyclonal Antibody to Tenascin-W
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Cytoskeleton & Extracellular Matrix Other Products
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ALX-210-894-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY: ≥90%
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In TRIS/glycine, pH 8.0, containing 0.01% sodium azide.
IMMUNOGEN: Recombinant FNIII repeats 7-9 of mouse tenascin-W.
SPECIFICITY: Recognizes human, mouse and rat tenascin-W.
APPLICATION: ELISA (1:10’000)
Immunocytochemistry (1:1’000)
Immunohistochemistry (frozen sections, paraffin sections (1:500))
Immunoprecipitation
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Murine tenascin-W: a novel mammalian tenascin expressed in kidney and at sites of bone and smooth muscle development: A. Scherberich, et al.; J. Cell Sci. 117, 571 (2004) Abstract; Full Text
Tenascin-W is found in malignant mammary tumors, promotes alpha8 integrin-dependent motility and requires p38MAPK activity for BMP-2 and TNF-alpha induced expression in vitro: A. Scherberich, et al.; Oncogene 24, 1525 (2005) Abstract
General Literature References
Tenascins: R. Chiquet-Ehrismann; Int. J. Biochem. Cell Biol. 36, 986 (2004) Abstract
Connective tissues: signalling by tenascins: R. Chiquet-Ehrismann and R.P. Tucker; Int. J. Biochem. Cell Biol. 36, 1085 (2004) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies • Tumor Markers
 
 
ALX-210-898 Revised 11-Sep-07
Polyclonal Antibody to VASP (human)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein (human) PAb
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP / Related Products
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ALX-210-898-R100   100 µl 225.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant human VASP (vasodilator stimulated phosphoprotein) fused to a His-tag.
SPECIFICITY: Recognizes unphosphorylated as well as phosphorylated (Ser157) human VASP. Does not cross-react with mouse or rat VASP. For the detection of mouse VASP use Prod. No. ALX-210-880.
APPLICATION: Immunocytochemistry (1:500-1:‘000)
Western Blot (1:1’500-1:3’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).
Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
Product Images
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General Information
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens. 
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-056 Revised 23-Apr-07
Polyclonal Antibody to Myosin Regulatory Light Chain 2 (Atrial Isoform) (phosphorylated) (pSer22/23)
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SYNONYMS anti-MLC2a (phosphorylated) PAb (pSer22/23)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Myosin Antibodies
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ALX-215-056-R050   50 µl 210.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
SOURCE/HOST: From rabbit.
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In phosphate buffer containing 1% BSA and 0.01% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 15-27 (T15KQAQRGSSNVFS27) of myosin regulatory light chain 2 (atrial isoform) (MLC2a) phosphorylated at Ser22 and/or Ser23.
SPECIFICITY: Recognizes human, mouse, rat, monkey and bovine MLC2a phosphorylated at Ser22 and/or Ser23. Detects a band of ~20kDa by Western blot.
APPLICATION: Western Blot (1:5'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Add one volume of glycerol to the working aliquots for storage at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The MLCK-mediated alpha1-adrenergic inotropic effect in atrial myocardium is negatively modulated by PKCepsilon signaling: M. Grimm, et al.; Br. J. Pharmacol. 148, 991 (2006) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-057 Revised 23-Apr-07
Polyclonal Antibody to Myosin-binding Protein C (Cardiac Type) (phosphorylated) (pSer282)
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SYNONYMS anti-cMyBP-C (phosphorylated) PAb (pSer282)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Myosin Antibodies
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ALX-215-057-R050   50 µl 210.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
SOURCE/HOST: From rabbit.
PURITY DETAIL: Affinity-purified.
FORMULATION: Liquid. In phosphate buffer, pH 7.4, containing 0.1% BSA and 0.01% thimerosal.
IMMUNOGEN: Synthetic peptide corresponding to aa 276-288 (G276AGRRTpSDSHEDA288) of myosin-binding protein 2 (cardiac type) (cMyBP-C) phosphorylated at Ser282.
SPECIFICITY: Recognizes human, mouse, rat and dog cMyBP-C phosphorylated at Ser282. Detects a band of ~150kDa by Western blot.
APPLICATION: Western Blot (1:1'000-1:5’000)
Immunocytochemistry (1:200)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Molecular determinants of altered Ca2+ handling in human chronic atrial fibrillation: A. El-Armouche, et al.; Circulation 114, 670 (2006) Abstract; Full Text
General Information
Myosin-binding protein 2 (cardiac type) (cMyBP-C) is a protein located in the C-zones of the A-band of the sarcomere and interacts specifically with myosin, titin and actin. Mutations in the human cMyBP-C gene are one of the most frequent causes of familial hypertrophic cardiomyopathy. cMyBP-C can be phosphorylated at three different sites by a cAMP-regulated protein kinase (PKA) and a Ca2+<-calmodulin kinase (CAMK) bound to the thick filament. The dephosphorylated form binds to the S2 subfragment of myosin whereas the phosphorylated form binds to actin. In solution, phosphorylation of cMyBP-C increases force of contraction, but both phospho- and dephosphorylated forms of cMyBP-C stimulate the actin-activated myosin ATPase activity.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-260-120 Revised 12-Dec-07
Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Substrates
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ALX-260-120-M005   5 mg 250.00 USD Add To Cart
Product Specification
SEQUENCE: 2,4-Dinitrophenyl-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH
FORMULA: C44H61N13O13S
MW: 1012.1
PURITY: ≥95% (HPLC)
APPEARANCE: Yellow solid
SOLUBILITY: Soluble in DMSO; dilute with distilled water or buffer.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
Product Description
Fluorogenic substrate for MMP-2 and MMP-9. Excitation: 280nm; emission 360nm.
Product Specific Literature References
Continuously recording fluorescent assays optimized for five human matrix metalloproteinases: S. Netzel-Arnett, et al.; Anal. Biochem. 195, 86 (1991) Abstract
 
 
ALX-260-121 Revised 08-Jul-05
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Substrates
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ALX-260-121-M001   1 mg 190.00 USD Add To Cart
Product Specification
MW: 1680.0
PURITY: ≥97%
APPEARANCE: Yellow solid.
SOLUBILITY: DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry. Protect from light.
Product Description
Fluorogenic substrate for MMP-3 and MMP-9.
 
 
ALX-260-122 Revised 12-Jun-03
Mca-Pro-Cha-Gly-Nva-His-Ala-Dap(Dnp)-NH2