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Cancer
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ALX-201-203 Revised 23-Jul-07
Von Hippel-Lindau Disease Tumor Suppressor (β-domain) (human) (recombinant) (His)
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SYNONYMS VHL Disease Tumor Suppressor (β-domain) (human) (recombinant) (His)
PRODUCT LINE Cancer
PRODUCT CATEGORY Tumor Suppressors / Related Products
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ALX-201-203-C100   100 µg 220.00 USD Add To Cart
Product Specification
MW: ~19.2kDa.
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 2mM EDTA.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Gamma synuclein, a novel heat-shock protein-associated chaperone, stimulates ligand-dependent estrogen receptor alpha signaling and mammary tumorigenesis: Y. Jiang, et al.; Cancer Res. 64, 4539 (2004) Abstract
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-204/1 Revised 06-Jun-08 New product
pro-Bone Morphogenetic Protein 2 (human) (recombinant)
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SYNONYMS proBMP-2 (human) (recombinant)
PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Bone Morphogenetic Proteins [BMPs] & Receptors / Related Products
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ALX-201-204/1-C010   10 µg 380.00 USD Add To Cart
Product Specification
MW: ~85.5kDa.
SOURCE/HOST: Produced in E. coli and renatured from inclusion body material.
CONCENTRATION: 100μg/ml after reconstitution.
PURITY: ≥98% (SDS-PAGE, UV, MS)
FORMULATION: Lyophilized from 0.1M K-HEPES, pH 7.0, containing 3% mannitol.
ENDOTOXIN CONTENT: <0.02EU/mg protein.
RECONSTITUTION: Reconstitute with 100μl distilled water at +4°C for 30 minutes.
BIOLOGICAL ACTIVITY: Administration leads to ectopic bone formation in rats.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: The reconstituted solution can be diluted into other aqueous buffers and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor : F. Hillger, et al.; J. Biol. Chem. 280, 14974 (2005) Abstract; Full Text
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-205/1 Revised 17-Jun-08 New product
Bone Morphogenetic Protein 2 (human) (recombinant)
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SYNONYMS BMP-2 (human) (recombinant)
PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Bone Morphogenetic Proteins [BMPs] & Receptors / Related Products
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ALX-201-205/1-C010   10 µg 280.00 USD Add To Cart
Product Specification
MW: ~25.9kDa.
SOURCE/HOST: Produced in E. coli and renatured from inclusion body material.
CONCENTRATION: 100-500μg/ml after reconstitution. 
PURITY: ≥98% (SDS-PAGE, UV, MS)
FORMULATION: Lyophilized.
ENDOTOXIN CONTENT: <0.02EU/mg protein.
RECONSTITUTION: Reconstitute with 20-100μl 50mM sodium acetate solution, pH 4.3.
BIOLOGICAL ACTIVITY: Induces alkaline phosphatase expression at an EC50 = 14nM in C2C12 cells.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY:

The reconstituted solution can be further diluted in sodium acetate solution and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor: F. Hillger, et al.; J. Biol. Chem. 280, 14974 (2005) Abstract; Full Text
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-206 Revised 10-Feb-05
Melanoma Inhibitory Activity Protein (human) (recombinant)
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SYNONYMS MIA (human) (recombinant)
PRODUCT LINE Cancer
PRODUCT CATEGORY Antitumor Reagents Other Products
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ALX-201-206-C020   20 µg 490.00 USD Add To Cart
Product Specification
MW: ~12.2kDa.
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM potassium phosphate, pH 7.5, containing 150mM potassium chloride.
ENDOTOXIN CONTENT: <0.05EU/µg protein.
BIOLOGICAL ACTIVITY: Inhibits tumor growth in vitro.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for up to 5 weeks after receipt when stored at +4°C and for at least 1 year when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
General Information
MANUFACTURER Manufactured by Scil Proteins GmbH.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-207 Revised 14-Oct-08
pro-β-Nerve Growth Factor (human) (recombinant)
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SYNONYMS pro-β-NGF (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Nerve Growth Factors [NGF] & Receptors / Related Products
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ALX-201-207-C020   20 µg 490.00 USD Add To Cart
Product Specification
MW: ~49.7kDa.
SOURCE/HOST: Produced in E. coli.
CONCENTRATION:

PURITY: ≥98% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 7.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT: <0.05EU/µg protein.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of TF1 cells (EC50=130±30pM).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for up to 5 weeks after receipt when stored at +4°C and for at least 1 year when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Scil Proteins GmbH.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-208 Revised 12-Feb-08
β-Nerve Growth Factor (human) (recombinant)
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SYNONYMS β-NGF (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Nerve Growth Factors [NGF] & Receptors / Related Products
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ALX-201-208-C020   20 µg 190.00 USD Add To Cart
Product Specification
MW: ~26.5kDa.
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: ~0.65mg/ml.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 7.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT: <0.05EU/µg protein.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of TF1 cells (EC50=10.9±2.7pM).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for up to 5 weeks after receipt when stored at +4°C and for at least 1 year when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P01138: β-Nerve Growth Factor (human)
MANUFACTURER Manufactured by Scil Proteins GmbH.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-221 Revised 02-Oct-06
Pin1 (human) (recombinant)
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SYNONYMS Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant)
PPIase Pin1 (human) (recombinant)
Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant)
PPIase NIMA-interacting 1 (human) (recombinant)
Rotamase Pin1 (human) (recombinant)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-201-221-C100   100 µg 325.00 USD Add To Cart
Product Specification
MW: ~18.2kDa
SOURCE/HOST: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products
 
 
ALX-201-234 Revised 07-Nov-07
Proliferating Cell Nuclear Antigen (human) (recombinant)
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SYNONYMS PCNA (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Proliferating Cell Nuclear Antigen [PCNA] / Related Products
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ALX-201-234-C025   25 µg 240.00 USD Add To Cart
Product Specification
MW: 87kDa (homotrimer of 29kDa)
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 50mM sodium chloride, 1mM DTT and 1mM EDTA.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 4 weeks when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The solution structure of functionally active human proliferating cell nuclear antigen determined by small-angle neutron scattering: P. Schurtenberger, et al.; J. Mol. Biol. 275, 123 (1998) Abstract
General Literature References
Proliferating cell nuclear antigen (PCNA): a dancer with many partners: G. Maga and U. Hubscher; J. Cell Sci. 116, 3051 (2003) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Tumor Markers
 
 
ALX-201-239 Revised 12-Nov-08
Procathepsin K (human) (recombinant)
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SYNONYMS Procathepsin O (human) (recombinant)
Procathepsin O2 (human) (recombinant)
Procathepsin X (human) (recombinant)
PRODUCT LINE Cancer
PRODUCT CATEGORY Cathepsins
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ALX-201-239-C010   10 µg 166.00 USD Add To Cart
Product Specification
MW: ~35kDa
EC: 3.4.22.38
SOURCE/HOST: Produced in E. coli (aa 19-329). Methionine was introduced at aa 18 to create a new N-terminal sequence (MEEIL).
CONCENTRATION: 150μg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS, pH 8.0, containing 500mM sodium chloride.
SPECIFIC ACTIVITY: ≥1’000mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol of substrate per min. at 37°C, pH 5.5.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for several months when stored at -80°C. After activation, cathepsin is highly autoproteolytic. When not used immediately, add 1mM MMTS for stabilization and shock freeze in liquid nitrogen . Do not store dilutions of the enzyme.
HANDLING: Avoid freeze/thaw cycles.
Product Description
Member of the papain cysteine proteinase family identified as the predominant proteinase responsible for the resorption of the bone matrix.
Product Specific Literature References
[1] Reversible modification of the sulfhydryl groups of Escherichia coli succinic thiokinase with methanethiolating reagents, 5.5’-Dithio-bis(2-nitrobenzoic acid), p-hydroxymercuribenzoate, and ethylmercurithiosalicylate: J.S. Nishimura, et al.; Arch. Biochem. Biophys. 170, 461 (1975) Abstract
[2] Autocatalytic activation of human cathepsin K: M.S. McQueney, et al.; J. Biol. Chem. 272, 13955 (1997) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Activity:
Activity of mature protein was measured in 50mM MES, pH 5.5, containing 2.5mM EDTA and 5mM DTT. Assay was initiated by addition of substrate Z-Phe-Arg-AMC (Prod. No. ALX-260-131) to a final concentration of 100µM (stock solution 10mM in DMSO). Activity was monitored by the increase of fluorescence accompanying release of the product 7-amino-4-methylcoumarin (AMC) (excitation wavelength of 360nm; emission wavelength of 460nm) (see [2]).

Activation:
Activate by adjusting procathepsin K to pH 4.0 by adding an equal volume of 100mM sodium acetate, pH 3.9, containing 10mM DTT, 5mM EDTA and incubate for 40 min. at room temperature. MMTS (methyl methanethiosulfonate) modifies cysteines by attaching its relatively small, uncharged thiomethyl blocking group to reactive sulfhydryl groups (see [1]). This reversible reaction arrests the autoproteolytical process. Activity can be restored to nearly unmodified level by adding L-cysteine (~3M excess over MMTS).

Inhibitors:
Cathepsin K is inhibited by leupeptin (Prod. No. ALX-260-009) (IC50=70nM), E-64 (IC50=5nM) or cystatine. Minimal effects exhibit pepstatin, phenylmethylsulfonyl fluoride (Prod. No. ALX-270-184), inhibitors of aspartyl and serine proteases. No inhibition was observed by addition of EDTA or phenanthroline, classical inhibitors of metalloproteases.

Swiss-Prot link P43235: Cathepsin K (human) (precursor)
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Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins • Bone Metabolism Other Products
 
 
ALX-201-240 Revised 16-Sep-08
Elafin (human) (recombinant)
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SYNONYMS ESI (human) (recombinant)
Elastase-specific Inhibitor (human) (recombinant)
SKALP (human) (recombinant)
Skin-derived Antileukoproteinase (human) (recombinant)
Protease Inhibitor WAP3 (human) (recombinant)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antimicrobial Peptides & Proteins / Related Products
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ALX-201-240-C100   100 µg 275.00 USD Add To Cart
Product Specification
MW: ~5.9kDa
SOURCE/HOST: Produced in yeast.
PURITY: ≥90% (HPLC, SDS-PAGE, MS)
APPEARANCE: White to faint yellow powder.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute in sterile distilled water. Further dilutions should be made in aqueous buffers, pH 4.0-8.0.
BIOLOGICAL ACTIVITY: Inhibits human leukocyte elastase (Ki=0.17nM) and human proteinase 3 (Ki=0.42nM) .
SPECIFIC ACTIVITY: Inhibition of human leukocyte elastase: ≥90%
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
HAZARD: HARMFUL.
Product Description
Belongs to the chelonianin family of protease inhibitors and is involved in the physiological control of neutrophil serine proteinases, where it targets NE and proteinase 3. Shows antiprotease, antibacterial and antiinflammatory activity and primes innate immunity.
Product Specific Literature References
[1] Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence: O. Wiedow, et al.; J. Biol. Chem. 265, 14791 (1990) Abstract; Full Text
[2] Skin-derived antileucoproteases (SKALPs): characterization of two new elastase inhibitors from psoriatic epidermis: J. Schalkwijk, et al.; Br. J. Dermatol. 122, 631 (1990) Abstract
[3] Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor: J.M. Sallenave and A.P. Ryle; Biol. Chem. Hoppe Seyler 372, 13 (1991) Abstract
[4] Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment: Saheki, et al.; Biochem. Biophys. Res. Commun. 185, 240 (1992) Abstract
[5] Kinetics of the inhibition of human leukocyte elastase by elafin, a 6-kilodalton elastase-specific inhibitor from human skin: Q.L. Ying and S.R. Simon; Biochemistry 32, 1866 (1993) Abstract
[6] SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking: H.O. Molhuizen, et al.; J. Biol. Chem. 268, 12028 (1993) Abstract
[7] Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions: J.M. Sallenave and A. Silva; Am. J. Respir. Cell. Mol. Biol. 8, 439 (1993) Abstract
[8] Kinetics of the inhibition of human leukocyte elastase by elafin, a 6-kilodalton elastase-specific inhibitor from human skin: Q.L. Ying and S.R. Simon; Biochemistry 32, 1866 (1993) Abstract
[9] Elastase inhibitor elafin is a new type of proteinase inhibitor which has a transglutaminase-mediated anchoring sequence termed "cementoin": K. Nara, et al.; J. Biochem. (Tokyo) 115, 441 (1994) Abstract
[10] Accelerated evolution in inhibitor domains of porcine elafin family members: I. Tamechika, et al.; J. Biol. Chem. 271, 7012 (1996) Abstract; Full Text
[11] Identification and sequence analysis of two new members of the SKALP/elafin and SPAI-2 gene family. Biochemical properties of the transglutaminase substrate motif and suggestions for a new nomenclature: P.L. Zeeuwen, et al.; J. Biol. Chem. 272, 20471 (1997) Abstract; Full Text
[12] The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a C-terminal four-disulphide core: J. Schalkwijk, et al.; Biochem. J. 340, 569 (1999) Abstract; Full Text
[13] The role of secretory leukocyte proteinase inhibitor and elafin (elastase-specific inhibitor/skin-derived antileukoprotease) as alarm antiproteinases in inflammatory lung disease: J.M. Sallenave; Respir. Res. 1, 87 (2000) Abstract
[14] Kinetics of the inhibition of proteinase 3 by elafin: Q.L. Ying and S.R. Simon; Am. J. Respir. Cell Mol. Biol. 24, 83 (2001) Abstract
[15] Trappin ovine molecule (TOM), the ovine ortholog of elafin, is an acute phase reactant in the lung: T.I. Brown, et al.; Physiol. Genomics 19, 11 (2004) Abstract; Full Text
[16] Elafin and Its Precursor Trappin-2 Still Inhibit Neutrophil Serine Proteinases when They Are Covalently Bound to Extracellular Matrix Proteins by Tissue Transglutaminase: N. Guyot, et al.; Biochemistry 44, 15610 (2005) Abstract
[17] SLPI and elafin: one glove, many fingers: S.E. Williams, et al.; Clin. Sci. (Lond) 110, 21 (2006) Abstract
Further Categories Containing This Product:
Proteases Other Products • Recombinant Proteins / Fusion Proteins • Inflammation Other Products
 
 
ALX-201-241 Revised 18-Mar-08
TPD52L1 (human) (recombinant) (His)