ALEXIS Biochemicals: Polyclonal Antibody to CREB Binding Protein

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ALX-201-279

Revised 18-Mar-08

CREB Binding Protein (mouse) (recombinant) (His)
SYNONYMS	CBP (mouse) (recombinant) (His)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	CREB & CREB Binding Protein / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-279-C002		2 µg	280.00 USD

Product Specification

EC:	EC 2.3.1.48
SOURCE/HOST:	Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION:	0.5mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY:	~50-100ng are required for standard HAT assay.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P45481: CREB Binding Protein (mouse)

Further Categories Containing This Product:

Histone Acetyl- & Methyl-Transferases / Related Products • Recombinant Proteins / Fusion Proteins

ALX-804-389

Revised 30-May-08

Monoclonal Antibody to CREB (pSer133) and ATF1 (pSer63) (phosphorylated) (10E9)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	CREB & CREB Binding Protein / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-389-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	10E9
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide corresponding to aa 125-135 of CREB phosphorylated at Ser¹³³.
SPECIFICITY:	Recognizes the RRPpSYRK motif of human, mouse or dog CREB phosphorylated at Ser¹³³ and ATF1 phosphorylated at Ser⁶³. Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot.
APPLICATION:	Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:	yes

Further Categories Containing This Product:

Phospho-specific Antibodies • Monoclonal Antibodies

ALX-210-229

Revised 23-Feb-05

Polyclonal Antibody to CREB Binding Protein
SYNONYMS	anti-CBP PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	CREB & CREB Binding Protein / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-229-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
FORMULATION:	Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 162-176 (A¹⁶²TSSPATSQTGPGIC¹⁷⁶) in the nuclear factor binding domain of human CBP (CREB binding protein).
SPECIFICITY:	Recognizes human and mouse CBP. Detects a band of ~265kDa by Western blot.
APPLICATION:	Western Blot (1-2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-153-023.

General Information

CREB binding protein (CBP) is a transcriptional coactivator closely related to p300, interacting with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), cFos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB, and MyoD. CBP displays histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.