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Biotinylated Reagents Other Products
You are here: Product Lines > Immunology > Immunological Methods > Biotin-(Strept)avidin Systems / Related Products > Biotinylated Reagents > Biotinylated Reagents Other Products
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ALX-350-267 Revised 08-Apr-08
Phalloidin (Biotin)
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SYNONYMS Biotinyl-hexamethylenamidosuccinyl-aminomethyldithiolano-phalloidin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products for Cytoskeletal Research
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ALX-350-267-MC01   0.1 mg 175.00 USD Add To Cart
Product Specification
FORMULA: C57H83N13O13S4
MW: 1286.6
SOURCE/HOST: Semisynthetic from aminomethyldithiolano-phalloidin.
PURITY: ≥90%
APPEARANCE: Amorphous solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Prepare fresh solutions for each application.
HANDLING: Protect from light and moisture.
HAZARD: VERY TOXIC.

Product Description
Binds actin filaments and streptavidin simultaneously, due to the long spacer between the two active components.
Product Specific Literature References
Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis: H. Faulstich, et al.; Exp. Cell Res. 144, 73 (1983) Abstract
Biotinylphallotoxins: preparation and use as actin probes: H. Faulstich, et al.; J. Histochem. Cytochem. 37, 1035 (1989) Abstract
Further Categories Containing This Product:
Amanitins & Phalloidins / Related Products • Actin / Related Products • Biotinylated Reagents Other Products
 
 
ALX-581-150 Revised 16-Sep-08 New product
LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Biotin)
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SYNONYMS Lipopolysaccharide from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Biotin)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
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ALX-581-150-R500   500 µl 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method [1]. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered.
CONCENTRATION: 1mg/ml
PURITY: Absence of dectectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double-distilled water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Use must be restricted to qualified personnel. Keep sterile. Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Description
Useful reagent for evaluating LPS binding and cell activation in white blood cells.
Product Specific Literature References
[1] Newer aspects of the chemsitry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component: C. Galanos, et al.; Int. Rev. Biochem. 14, 239 (1977)
[2] Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients: R. Salomao, et al.; J. Endotoxin Res. 8, 371 (2002) Abstract
[3] Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood: M.K. Brunialti, et al.; Cytometry 50, 14 (2002) Abstract; Full Text
[4] Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein: D. Diament, et al.; Infect. Immun. 70, 1677 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: As the biotinylation reagent and standard biotinylation protocol favors protein/peptide (contaminants) about 1000-fold over sugar as a target, it is mandatory to use >99.9% pure LPS to exclude the biotinylation of contaminants. These are usually present in many commercial LPS preparations and would lead to misleading observations of the tracking/staining with the reagents.
TLRgrade™ LPS fulfills such high quality requirements and the biotinylation procedure is chosen to preserve TLR4 (Toll-like receptor 4) binding and cellular activation using TLRgrade™ LPS-Biotin.

Related Product:

- For the unconjugated LPS, please see LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Prod. No. ALX-581-009).
Related Products
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-610-035 Revised 03-Feb-05
DY-630-biotin
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-035-C500   500 µg 140.00 USD Add To Cart
Product Specification
FORMULA: C52H74N6O9S2
MW: 991.3
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Fluorescent label. Ex(max): ~630nm (red spectral region).
General Information
MANUFACTURER Manufactured by Dyomics GmbH.
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-610-042 Revised 03-Feb-05
DY-550-biotin
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-042-C500   500 µg 140.00 USD Add To Cart
Product Specification
FORMULA: C48H70N7O12S2Na
MW: 1024.2
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Fluorescent label. Ex(max): ~550nm.
General Information
MANUFACTURER Manufactured by Dyomics GmbH.
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Biotinylated Reagents Other Products
 
 
ALX-209-001 Revised 25-May-07
Calmodulin (bovine brain) (high purity) (Biotin)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calmodulins
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ALX-209-001-C050   50 µg 180.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from bovine brain.
CONCENTRATION: 0.5mg/ml.
FORMULATION: Liquid. In PBS containing 0.1% BSA and 0.02% sodium azide.
APPLICATION: Detection of calmodulin-binding proteins by Western blot with streptavidin-alkaline phosphatase conjugates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase: R.L. Kincaid, et al.; Meth. Enzymol. 159, 605 (1988) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P02593: Calmodulin (human)
AfCS Signalling Gateway link A000452: Calmodulin I (mouse)
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-209-002 Revised 02-Aug-06
Annexin V (human) (recombinant) (Biotin)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-002-T020   20 tests 106.00 USD Add To Cart
ALX-209-002-T100   100 tests 352.00 USD Add To Cart
ALX-209-002-T300   300 tests 531.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Biotinylated Reagents Other Products
 
 
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