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APO-54N-035 Revised 10-Oct-08
MDA5 (human) (IntraCellular) Detection Set [For ELISA Application]
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SYNONYMS Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) Detection Set [For ELISA Application]
Helicard (human) (IntraCellular) Detection Set [For ELISA Application]
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-035-KI01   1 Set 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0 to 32ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Description

This intracellular Detection Set is for measuring protein levels in cell lysates.  It allows to quantitatively determine:
a. Levels of MDA5
b. Interferon response since MDA5 is an interferon inducible gene
b. Virus infection response
General Information
The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard; Ifih1) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belongs to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS (mitochondrial antiviral signalling), IPS-1 (interferon-β promoter stimulator 1) or VISA (virus-induced signalling adapter) [6, 7]. Cardif/ MAVS/ IPS-1/ VISA then interacts with several TRAFs molecules, leading to the activation of regulatory kinases, such as TBK1, IKKε , and the IKKα/β/γ complex. These kinases activate respective transcription factors, IRF-3, IRF-7, and NF-κB that participate in the activation of IFN and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA while only poly I: C acts as a chemical agonist for MDA5. The physiological agonist of MDA5 has still to be discovered. Mda-5 is an early response gene inducible by IFN and tumor necrosis factor-α, responding predominantly to IFN-β [8].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9BYX4: MDA5 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[8] mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract; Full Text
[6] Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
[7] IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
[1] Recognition of viruses by innate immunity: O. Takeuchi & S. Akira; Immunol. Rev. 220, 214 (2007) Abstract
[4] Cytoplasmic recognition of RNA: M. Yoneyama, et al.; Adv. Drug Deliv. Rev. 60, 841 (2008) Abstract
[5] Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses: T. Venkataraman, et al.; J. Immunol. 178, 6444 (2007) Abstract
[2] MDA5/RIG-I and virus recognition: O. Takeuchi & S. Akira; Curr. Opin. Immunol. 20, 17 (2008) Abstract
[3] TLR3: Interferon induction by double-stranded RNA including poly(I:C): M. Matsumoto & T. Seya; Adv. Drug Deliv. Rev. 60, 805 (2008)
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • ELISA Sets
 
 
APO-54N-039 Revised 10-Oct-08 New product
MDA5 (human) (IntraCellular) ELISA Kit
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SYNONYMS Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) ELISA Kit
Helicard (human) (IntraCellular) ELISA Kit
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-039-KI01   1 Kit 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0.5 to 32ng/ml).
QUANTITY: For 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • ELISA & EIA Kits
 
 
ALX-804-863 Revised 29-Jan-08
Monoclonal Antibody to MDA5 (human) (Hely-1)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (human) MAb (Hely-1)
anti-Helicard (human) MAb (Hely-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-863-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Hely-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY: Recognizes human MDA5.
APPLICATION: ELISA
Immunoprecipitation (1:100)
Western Blot (1:1‘000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006) Abstract
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Further Categories Containing This Product:
Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Interferons & Interferon Receptors Other Products • Monoclonal Antibodies
 
 
ALX-804-849 Revised 16-Aug-07
Monoclonal Antibody to RIG-I (Alme-1)
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SYNONYMS anti-RIG-1 MAb (Alme-1)
anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1)
anti-DEAD-box Protein 58 MAb (Alme-1)
anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-849-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Alme-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human and mouse RIG-I.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
Related Products
Further Categories Containing This Product:
Antiviral Signalling / Related Products • NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-963 Revised 27-Nov-08 New product
Polyclonal Antibody to LGP2 (human) (AT137)
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SYNONYMS anti-Probable ATP-dependent RNA Helicase DHX58 (human) PAb (AT137)
anti-Probable ATP-dependent RNA Helicase LGP2 (human) PAb (AT137)
anti-Protein D11Lgp2 Homolog (human) PAb (AT137)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-963-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to 15 aa near the C-terminus of human LGP2.
SPECIFICITY: Recognizes human LGP2.
APPLICATION: Immunoprecipitation (1:200)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MDA5 and RIG-I are two key intracellular receptors that recognize virus infection to produce type I IFN. LGP2 is homologous to MDA5 and RIG-I, but lacks the caspase recruitment domain (CARD) crucial for their antiviral activity [1]. LGP2 may be a negative regulator of host innate immune defense against viruses by acting through dsRNA sequestration or inhibition of RIG-I dimerization [2].
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity: M. Yoneyama, et al.; J. Immunol. 175, 2851 (2005) Abstract; Full Text
[2] Negative regulation of cytoplasmic RNA-mediated antiviral signaling: A. Komuro, et al.; Cytokine 43, 350 (2008) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-935 Revised 20-Feb-08
Polyclonal Antibody to MDA5 (human) (AT113)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (human) PAb (AT113)
anti-Helicard (human) PAb (AT113)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-935-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY: Recognizes human MDA5.
APPLICATION: Immunoprecipitation (1:100)
Western Blot (1:1‘000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Related Products
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products • NF-kB Pathway Other Products • Interferons & Interferon Receptors Other Products • Antiviral Signalling / Related Products • Polyclonal Antibodies
 
 
ALX-210-352 Revised 30-Oct-06
Polyclonal Antibody to MDA5 (mouse) (AL180)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (mouse) PAb (AL180)
anti-Helicard (mouse) PAb (AL180)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-352-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).
SPECIFICITY: Recognizes mouse MDA5.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-932 Revised 28-Feb-07
Polyclonal Antibody to RIG-I (human) (AT111)
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SYNONYMS anti-RIG-1 (human) PAb (AT111)
anti-Retinoic Acid-inducible Gene 1 Protein (human) PAb (AT111)
anti-DEAD-box Protein 58 (human) PAb (AT111)
anti-Probable ATP-dependent RNA Helicase DDX58 (human) PAb (AT111)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-932-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human RIG-I.
APPLICATION: Immunoprecipitation (1:100)
Western blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
Related Products