ALEXIS Biochemicals: Cu/Zn-Superoxide Dismutase ELISA Kit

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ALX-430-069

Revised 31-Oct-08

MnTBAP chloride
SYNONYMS	Manganese (III) tetrakis (4-benzoic acid)porphyrin chloride
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Porphyrins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-069-M010		10 mg	20.00 USD
ALX-430-069-M050		50 mg	60.00 USD

Product Specification

FORMULA:	C₄₈H₂₈MnN₄O₈Cl
MW:	879.2
PURITY:	≥97% (EA, MS)
APPEARANCE:	Black crystalline powder.
SOLUBILITY:	Dissolve at 0.1M in aqueous base, then add buffer to lower pH to pH 7.0 or higher as needed.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light and moisture.

Product Description

Cell permeable SOD mimetic. Potent inhibitor of peroxynitrite-induced oxidative reactions (peroxynitrite scavenger), but not a scavenger of nitric oxide (NO).

Product Specific Literature References

Stable Mn(III) porphyrins mimic superoxide dismutase in vitro and substitute for it in vivo: K.M. Faulkner, et al.; J. Biol. Chem. 269, 23471 (1994) Abstract; Full Text
A metalloporphyrin superoxide dismutase mimetic protects against paraquat-induced endothelial cell injury, in vitro: B.J. Day, et al.; J. Pharm. Exp. Ther. 275, 1227 (1995) Abstract
Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger: C. Szabó, et al.; FEBS Lett. 381, 82 (1996) Abstract
Mitochondrial nitric-oxide synthase stimulation causes cytochrome c release from isolated mitochondria. Evidence for intramitochondrial peroxynitrite formation: P. Ghafourifar, et al.; J. Biol. Chem. 274, 31185 (1999) Abstract; Full Text
Reactive oxygen species regulate activation-induced T cell apoptosis:: D.A. Hildeman, et al.; Immunity 10, 735 (1999) Abstract
Doxorubicin-induced apoptosis in endothelial cells and cardiomyocytes is ameliorated by nitrone spin traps and ebselen.: S. Kotamraju, et al.; J. Biol. Chem. 275, 33585 (2000) Abstract; Full Text
Endothelial heme oxygenase-1 induction by hypoxia. Modulation by inducible nitric-oxide synthase and S-nitrosothiols: R. Motterlini, et al.; J. Biol. Chem. 275, 13613 (2000) Abstract; Full Text
Mitochondrial membrane permeabilization and superoxide production during apoptosis. A single-cell analysis: H. Dussmann, et al.; J. Biol. Chem. 278, 12645 (2003) Abstract; Full Text

Further Categories Containing This Product:

Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Superoxide Dismutases [SODs] / Related Products

ALX-430-070

Revised 05-Mar-08

MnTMPyP . pentachloride
SYNONYMS	Manganese (III) tetrakis (1-methyl-4-pyridyl)porphyrin . 5Cl^-
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Porphyrins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-070-M010		10 mg	20.00 USD
ALX-430-070-M050		50 mg	50.00 USD

Product Specification

FORMULA:	C₄₄H₃₆MnN₈Cl₅
MW:	909.0
PURITY:	≥95%
APPEARANCE:	Black crystalline powder.
SOLUBILITY:	Soluble in water or aqueous buffers (e.g. 0.1M TRIS-HCl, pH 9.0 containing 1mM EDTA (>50mg/ml) or PBS, pH 7.2 (4mg/ml)).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Prepare solutions immediately before experiments. We recommend using fresh preparations each day.

Product Description

Cell permeable SOD mimetic. Catalyzes the dismutation of O₂^- even in the presence of excess EDTA.

Product Specific Literature References

Stable Mn(III) porphyrins mimic superoxide dismutase in vitro and substitute for it in vivo: K.M. Faulkner, et al.; J. Biol. Chem. 269, 23471 (1994) Abstract; Full Text
A cationic manganic porphyrin inhibits uptake of paraquat by Escherichia coli: S.I. Liochev & I. Fridovich; Arch. Biochem. Biophys. 321, 271 (1995) Abstract
Superoxide scavenging by Mn(II/III) tetrakis (1-methyl-4-pyridyl) porphyrin in mammalian cells: P.R. Gardner, et al.; Arch. Biochem. Biophys. 325, 20 (1996) Abstract
The ortho effect makes manganese(III) meso-tetrakis(N-methylpyridinium- 2-yl)porphyrin a powerful and potentially useful superoxide dismutase mimic: Batinic-Haberle, et al.; J. Biol. Chem. 273, 24521 (1998) Abstract; Full Text
Molecular actions of a Mn(III)Porphyrin superoxide dismutase mimetic and peroxynitrite scavenger: reaction with nitric oxide and direct inhibition of NO synthase and soluble guanylyl cyclase: S. Pfeiffer, et al.; Mol. Pharmacol. 53, 795 (1998) Abstract
SODs are involved in the regulation of ICAM-1 expression in human melanoma and endothelial cells: R. Morandini, et al.; Cell. Mol. Biol. (Noisy-le-grand) 45, 1053 (1999) Abstract
Effects of superoxide dismutase mimetics on the activity of nitric oxide in rat aorta: A. MacKenzie, et al.; Br. J. Pharmacol. 127, 1159 (1999) Abstract
Inducible nitric oxide synthase-derived superoxide contributes to hypereactivity in small mesenteric arteries from a rat model of chronic heart failure: A.A. Miller, et al.; Br. J. Pharmacol. 131, 29 (2000) Abstract

Further Categories Containing This Product:

Antioxidants, Flavonoids & Free Radical Scavengers Other Products • NOS Inhibitors (NOS Induction & Enzyme Activity) • Superoxide Dismutases [SODs] / Related Products

ALX-202-022

Revised 01-Oct-07

Cu/Zn-Superoxide Dismutase (bovine liver)
SYNONYMS	SOD (Cu/Zn) (bovine liver)
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Superoxide Dismutases [SODs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-202-022-UT50		5 KU	40.00 USD
ALX-202-022-5T50		5x5 KU	160.00 USD
ALX-202-022-UZ50		50 KU	170.00 USD
ALX-202-022-5Z50		5x50 KU	660.00 USD

Product Specification

MW:	~31kDa.
CAS NUMBER:	9054-89-11
EC:	1.15.1.1
SOURCE/HOST:	Isolated from bovine liver.
FORMULATION:	Lyophilized. Essentially salt free.
RECONSTITUTION:	Reconstitute with distilled water, saline or neutral buffer to give a clear slightly blue solution.
SPECIFIC ACTIVITY:	~4'600U/mg protein. One unit is defined as the amount of enzyme that inhibits the rate of reduction of ferricytochrome c by 50% at 25°C, pH 7.8 (J.M. McCord & I. Fridovich; J. Biol. Chem. 244, 6049 (1969)).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted product should be stored at +4° and is stable for up to 2 days.
HANDLING:	Avoid freeze/thaw cycles.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P00442: Cu/Zn Superoxide Dismutase (bovine)

Further Categories Containing This Product:

Natural Proteins • Enzymes

ALX-804-265

Revised 25-Nov-08

Monoclonal Antibody to MnSOD (MnS-1)
SYNONYMS	anti-Manganese Superoxide Dismutase MAb (MnS-1)
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Superoxide Dismutases [SODs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-265-C100		100 µg	259.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat Others
CLONE:	MnS-1
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS.
IMMUNOGEN:	Human MnSOD (manganese superoxide dismutase).
SPECIFICITY:	Recognizes human MnSOD. Cross-reacts with rat and rabbit MnSOD.
APPLICATION:	Immunohistochemistry (frozen sections): 0.5-10µg/ml. Use to stain both indirectly or directly acetone-fixed cryostat sections or cell smears; recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedure or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. Western Blot. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-850-033

Revised 17-Apr-08

Cu/Zn-Superoxide Dismutase ELISA Kit
SYNONYMS	SOD (Cu/Zn) ELISA Kit
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Superoxide Dismutases [SODs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-033-KI01		1 Kit	619.00 USD

Product Specification

SENSITIVITY:	0.04ng/ml (range 0.08 to 5ng/ml)
QUANTITY:	96 wells (~80 tests).
APPLICATION:	For the quantitative determination of human Cu/Zn SOD in cell culture supernatants, serum and other body fluids.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Description

Late stages of apoptosis are characterized by the loss of membrane integrity and a release of the intracellular Cu/Zn SOD.

Product Specific Literature References

A highly sensitive non-radioactive cytotoxicity assay for human target cells: L. Franke & T. Porstmann; J. Immunol. Methods 171, 259 (1994) Abstract

General Information

MANUFACTURER

Manufactured by Bender MedSystems.

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