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Items 15 of 15
ALX-201-024
Revised 24-Feb-05
HSF1 (human) (recombinant)
SYNONYMS
Heat Shock Factor 1 (human) (recombinant)
PRODUCT LINE
Stress & Heat Shock Proteins
PRODUCT CATEGORY
Stress & Heat Shock Proteins Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-201-024-C150
150 µg
343.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Liquid. Sonicated
E. coli
extract at a total protein concentration of 6mg/ml in 25mM HEPES, pH 7.9, 12.5mM MgCl, 0.1mM EDTA, 10% glycerol, 1mM DTT, 0.1% NP-40 and 300mM KCl and protease inhibitors.
APPLICATION:
Positive control in gel shift assays (25 tests) or Western blot (50 tests) with PAb to HSF1 (Prod. No.
ALX-210-129
). DNase I footprinting and
in vitro
transcription assays.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-20°C
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q00613:
HSF 1 (human)
Further Categories Containing This Product:
Transcription Factors Other Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-199
Revised 11-Jan-07
CEBP-α (270-358) (human) (recombinant) (His)
SYNONYMS
CCAAT/Enhancer Binding Protein α (270-358) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
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ALX-201-199-C100
100 µg
220.00 USD
Product Specification
MW:
~14.5kDa.
SOURCE/HOST:
Produced in
E. coli.
bZip region of CEBP-α (aa 270-358).
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-200
Revised 04-Mar-05
CEBP-γ (39-147) (human) (recombinant) (His)
SYNONYMS
CCAAT/Enhancer Binding Protein γ (39-147) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-200-C100
100 µg
220.00 USD
Product Specification
MW:
~16.5kDa.
SOURCE/HOST:
Produced in
E. coli.
DNA binding domain of CEBP-γ (aa 39-147).
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-243
Revised 26-Apr-06
ETS1 (human) (recombinant)
SYNONYMS
c-ets-1 Protein (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
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ALX-201-243-C050
50 µg
210.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
2mg/ml
PURITY:
≥90% (SDS-PAGE)
APPEARANCE:
Liquid. In 50mM TRIS-KOH, pH 7.0, containing 6mM magnesium dichloride, 50mM potassium chloride, 5µM zinc dichloride, 2mM DTT and 0.1% NP40.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
USE/STABILITY:
For consistent results use a fresh aliquot for each experiment.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
The Ets family of transcription factors is known to control the expression of genes critical for cellular proliferation, differentiation, and transformation. They activate transcription by interaction with purine-rich sequences in promoter/enhancer regions of various genes. Differential expression of Ets genes is particularly associated with embryonic development of the central nervous system. Previous studies have implicated Ets transcription factors in apoptosis; however, exact mechanisms remain unclear. The mammalian Ets1 gene encodes a set of nuclear phosphoproteins ranging in size from 42 to 54kD.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-210-129
Revised 25-Jan-07
Polyclonal Antibody to HSF1
SYNONYMS
anti-Heat Shock Factor 1 PAb
PRODUCT LINE
Stress & Heat Shock Proteins
PRODUCT CATEGORY
Stress & Heat Shock Proteins Other Products
Ordering Information
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Format:
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ALX-210-129-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human HSF1 (heat shock factor 1).
SPECIFICITY:
Recognizes human, mouse and rat HSF1. Detects a band of ~83kDa by Western blot.
APPLICATION:
Gel Supershift Assay
Immunoprecipitation
Western Blot (1:10'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Transcription Factors Other Products
•
Polyclonal Antibodies
ALX-803-318
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (human) (R3-6E9)
SYNONYMS
anti-Runt-related Transcription Factor 3 (human) MAb (R3-6E9)
anti-CBF-α3 (human) MAb (R3-6E9)
anti-Core-binding Factor α3 Subunit (human) MAb (R3-6E9)
anti-AML-2 (human) MAb (R3-6E9)
anti-Acute Myeloid Leukemia 2 Protein (human) MAb (R3-6E9)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
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Unit Price:
Quantity:
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ALX-803-318-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
R3-6E9
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human RUNX3. Weakly cross-reacts with mouse RUNX3.
APPLICATION:
Immunohistochemistry (paraffin sections (2µg/ml)) (see [1])
Immunoprecipitation
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization:
K. Ito, et al.; Cancer Res.
65
, 7743 (2005)
Abstract
;
Full Text
[2]
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
[3]
Targets of genome copy number reduction in primary breast cancers identified by integrative genomics:
W. Chen, et al.; Genes Chromosomes Cancer
46
, 288 (2007)
Abstract
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-803-319
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (human) (R3-5G4)
SYNONYMS
anti-Runt-related Transcription Factor 3 (human) MAb (R3-5G4)
anti-CBF-α3 (human) MAb (R3-5G4)
anti-Core-binding Factor α3 Subunit (human) MAb (R3-5G4)
anti-AML-2 (human) MAb (R3-5G4)
anti-Acute Myeloid Leukemia 2 Protein (human) MAb (R3-5G4)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
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ALX-803-319-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
R3-5G4
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human RUNX3. Weakly cross-reacts with mouse RUNX3.
APPLICATION:
Immunoprecipitation
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization:
K. Ito, et al.; Cancer Res.
65
, 7743 (2005)
Abstract
;
Full Text
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-803-320
Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (R3-1E10)
SYNONYMS
anti-Runt-related Transcription Factor 3 MAb (R3-1E10)
anti-CBF-α3 MAb (R3-1E10)
anti-Core-binding Factor α3 Subunit MAb (R3-1E10)
anti-AML-2 MAb (R3-1E10)
anti-Acute Myeloid Leukemia 2 Protein MAb (R3-1E10)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-803-320-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
R3-1E10
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY:
Recognizes human and mouse RUNX3.
APPLICATION:
Immunohistochemistry (paraffin sections (2µg/ml) (see [1]))
Western Blot (0.2µg/ml (see [1]))
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis:
T. Yano, et al.; Mol. Cell. Biol.
26
, 4474 (2006)
Abstract
;
Full Text
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
General Literature References
Tumor suppressor activity of RUNX3:
S.C. Bae & J.K. Choi; Oncogene
23
, 4336 (2004)
Abstract
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer:
Q.C. Lau, et al.; Cancer Res.
66
, 6512 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products
•
Monoclonal Antibodies
ALX-804-221
Revised 22-Mar-06
Monoclonal Antibody to Ski (G8)
PRODUCT LINE
Cancer
PRODUCT CATEGORY
Tumor Markers
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-221-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE:
G8
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant v-Ski.
SPECIFICITY:
Recognizes cellular and hyperphosphorylated human, mouse, rat, chicken and
Xenopus
Ski
.
Detects bands of ~95kDa (Ski) and bands of up to ~115kDa (hyperphosphorylated Ski) by Western blot.
APPLICATION:
Immunocytochemistry (1-10µg/ml)
Immunoprecipitation (1-4µg per sample)
Western Blot (1-5µg/ml)
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-20°C
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski:
R. Nicol, et al.; Cell Growth Differ.
10
, 243 (1999)
Abstract
;
Full Text
Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents:
S.B. Cohen, et al.; Nucl. Acids Res.
27
, 1006 (1999)
Abstract
;
Full Text
General Information
Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF-β stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.
General Literature References
The ski oncogene:
P. Sutrave & S.H. Hughes; Oncogene
6
, 353 (1991), (Review)
Abstract
The Ski oncoprotein interacts with the Smad proteins to repress TGFbeta signaling:
K. Luo, et al.; Genes Dev.
13
, 2196 (1999)
Abstract
;
Full Text
SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling:
Y. Sun, et al.; PNAS
96
, 12442 (1999)
Abstract
;
Full Text
Viral ski inhibits retinoblastoma protein (Rb)-mediated transcriptional repression in a dominant negative fashion :
F. Tokitou, et al.; J. Biol. Chem.
274
, 4485 (1999)
Abstract
;
Full Text
Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor :
T. Nomura, et al.; Genes Dev.
13
, 412 (1999)
Abstract
;
Full Text
c-Ski acts as a transcriptional Co-repressor in transforming growth factor-beta signaling through interaction with smads :
S. Akiyoshi, et al. ; J. Biol. Chem.
274
, 35269 (1999)
Abstract
;
Full Text
Interaction of the Ski oncoprotein with Smad3 regulates TGF-beta signaling:
Y. Sun, et al.; Mol. Cell
4
, 499 (1999)
Abstract
Further Categories Containing This Product:
Transcription Factors Other Products
•
Monoclonal Antibodies
ALX-804-262
Revised 13-Jun-08
Monoclonal Antibody to LEF (2D12)
SYNONYMS
anti-Lymphoid Enhancer Factor MAb (2D12)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-804-262-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
2D12
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Recombinant LEF (lymphoid enhancer factor) (aa 1-85).
SPECIFICITY:
Recognizes human and mouse (weak) LEF. Detects a band of ~68kDa by Western blot. Does not cross-react with TCF3 (T cell factor 3) or TCF4 (T cell factor 4).
APPLICATION:
Immunocytochemistry
(use at 1µg/ml in PBS)
Western Blot
(1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
General Information
LEF is a transcription factor of the LEF/TCF protein family. LEF interacts with cytoplasmic β-catenin and regulates the expression of genes containing a LEF/TCF binding site. The formation of LEF-β-catenin heterodimers is an important step in the wnt/wg signal transduction pathway.
Further Categories Containing This Product:
Wnt Signalling Pathway Other Products
•
Monoclonal Antibodies
ALX-804-360
Revised 05-Oct-05
Monoclonal Antibody to SnoN (1G1)
SYNONYMS
anti-Ski-related Novel Protein Non-Alu-containing MAb (1G1)
PRODUCT LINE
Cancer
PRODUCT CATEGORY
Tumor Markers
Ordering Information
Product Numbers:
