ALEXIS Biochemicals: Ribosomal Protein S3 (human) (recombinant)

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ALX-840-606

Revised 08-Mar-07

Positive Control (Cell Lysate) for Antibodies to CARMA1 (human)
SYNONYMS	Positive Control (Cell Lysate) for Antibodies to CARD-containing MAGUK Protein 1 (human) Positive Control (Cell Lysate) for Antibodies to CARD11 (human) Positive Control (Cell Lysate) for Antibodies to Caspase Recruitment Domain-containing Protein 11 (human) Positive Control (Cell Lysate) for Antibodies to Bimp3 (human)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Positive Controls (Cell Lysates & Extracts)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-840-606-R100		100 µl	70.00 USD

Product Specification

SOURCE/HOST:	Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human CARMA1 (CARD-containing MAGUK protein 1) (aa 1-1147) fused at the C-terminus to a FLAG^®-tag.
QUANTITY:	100µl corresponding to 2x10⁵cells.
FORMULATION:	Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION:	Positive control in Western blot for PAb to CARMA1 (AL220) (Prod. No. ALX-210-903). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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General Information

BACKGROUND/TECHNICAL INFORMATION	FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products

ALX-522-143

Revised 24-Nov-08

IL-1RAcP (human):Fc (human) (recombinant)
SYNONYMS	Interleukin-1 Receptor Accessory Protein (human):Fc (human) (recombinant)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Interleukin-1 [IL-1] & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-143-C050		50 µg	390.00 USD

Product Specification

MW:	~80kDa
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human IL-1RAcP (aa 21-356) is fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Lonza).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9NPH3: IL-1RAcP (human)
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • NF-kB Pathway Other Products

APO-54N-035

Revised 10-Oct-08

MDA5 (human) (IntraCellular) Detection Set [For ELISA Application]
SYNONYMS	Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) Detection Set [For ELISA Application] Helicard (human) (IntraCellular) Detection Set [For ELISA Application]
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	RNA Helicases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-035-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.4ng/ml (range 0 to 32ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human MDA5 in cell extracts.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

This intracellular Detection Set is for measuring protein levels in cell lysates. It allows to quantitatively determine:
a. Levels of MDA5
b. Interferon response since MDA5 is an interferon inducible gene
b. Virus infection response

General Information

The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard; Ifih1) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belongs to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS (mitochondrial antiviral signalling), IPS-1 (interferon-β promoter stimulator 1) or VISA (virus-induced signalling adapter) [6, 7]. Cardif/ MAVS/ IPS-1/ VISA then interacts with several TRAFs molecules, leading to the activation of regulatory kinases, such as TBK1, IKKε , and the IKKα/β/γ complex. These kinases activate respective transcription factors, IRF-3, IRF-7, and NF-κB that participate in the activation of IFN and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA while only poly I: C acts as a chemical agonist for MDA5. The physiological agonist of MDA5 has still to be discovered. Mda-5 is an early response gene inducible by IFN and tumor necrosis factor-α, responding predominantly to IFN-β [8].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9BYX4: MDA5 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[8] mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract; Full Text
[6] Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
[7] IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
[1] Recognition of viruses by innate immunity: O. Takeuchi & S. Akira; Immunol. Rev. 220, 214 (2007) Abstract
[4] Cytoplasmic recognition of RNA: M. Yoneyama, et al.; Adv. Drug Deliv. Rev. 60, 841 (2008) Abstract
[5] Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses: T. Venkataraman, et al.; J. Immunol. 178, 6444 (2007) Abstract
[2] MDA5/RIG-I and virus recognition: O. Takeuchi & S. Akira; Curr. Opin. Immunol. 20, 17 (2008) Abstract
[3] TLR3: Interferon induction by double-stranded RNA including poly(I:C): M. Matsumoto & T. Seya; Adv. Drug Deliv. Rev. 60, 805 (2008)

Related Products

ALX-210-352	Polyclonal Antibody to MDA5 (mouse) (AL180)
ALX-210-935	Polyclonal Antibody to MDA5 (human) (AT113)
ALX-804-863	Monoclonal Antibody to MDA5 (human) (Hely-1)
APO-54N-035	MDA5 (human) (IntraCellular) Detection Set [For ELISA Application]

Further Categories Containing This Product:

ALX-804-137

Revised 07-Dec-05

Monoclonal Antibody to A20 (human) (8E8.38)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLRs Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-137-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	8E8.38
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Zinc finger domain corresponding to aa 375-537 of human A20.
SPECIFICITY:	Recognizes human A20.
APPLICATION:	Western Blot (1:1'000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles.

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Product Description

A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4 [1]. A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2 [2-4], which interfere with NF-kB activation upstream of the IKK complex.

Product Specific Literature References

A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome: F.S. Zetoune, et al.; Cytokine 15, 282 (2001) Abstract

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

[1] Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein: S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun. 3303, 586 (2003) Abstract
[2] The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN: K. Heyninck, et al.; J. Cell Biol. 145, 1471 (1999) Abstract; Full Text
[3] Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2: S. Van Huffel, et al.; J Biol Chem 276, 30216 (2001) Abstract; Full Text
[4] Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1: K. Heyninck, et al.; FEBS Lett. 536, 135 (2003) Abstract

Further Categories Containing This Product:

NF-kB Pathway Other Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products

ALX-804-134

Revised 01-Mar-07

Monoclonal Antibody to Bcl-10 (Carmen-1)
SYNONYMS	anti-CARMEN MAb (Carmen-1) anti-CIPER MAb (Carmen-1) anti-CLAP MAb (Carmen-1)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Caspase Recruitment Domain [CARD] Proteins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-134-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
CLONE:	Carmen-1
ISOTYPE:	Rat IgM
SOURCE/HOST:	Purified from concentrated hybridoma cell culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	>95% (SDS-PAGE)
PURITY DETAIL:	Purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Bcl-10.
SPECIFICITY:	Recognizes human and mouse Bcl-10.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored undiluted at +4°C.
HANDLING:	Do not freeze/thaw.

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General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

NF-kB Pathway Other Products • Innate Immunity Other Products • Monoclonal Antibodies

ALX-804-847

Revised 19-Jun-08

Monoclonal Antibody to Cardif (human) (Adri-1)
SYNONYMS	anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1) anti-IPS-1 Cardif (human) MAb (Adri-1) anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1) anti-MAVS (human) MAb (Adri-1) anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1) anti-VISA (human) MAb (Adri-1) anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antiviral Signalling / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-847-C100		100 µg	370.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Adri-1
ISOTYPE:	Mouse IgG2b
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY:	Recognizes human Cardif.
APPLICATION:	Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections) Immunoprecipitation (1:200) Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008) Abstract

General Information

RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract

Further Categories Containing This Product:

NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies

ALX-804-863

Revised 29-Jan-08

Monoclonal Antibody to MDA5 (human) (Hely-1)
SYNONYMS	anti-Melanoma Differentiation-associated Gene 5 (human) MAb (Hely-1) anti-Helicard (human) MAb (Hely-1)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	RNA Helicases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-863-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Hely-1
ISOTYPE:	Mouse IgG1
SOURCE/HOST:	Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY:	Recognizes human MDA5.
APPLICATION:	ELISA Immunoprecipitation (1:100) Western Blot (1:1‘000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006) Abstract