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Cell Death / Apoptosis / Autophagy
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ALX-201-065 Revised 18-Sep-08
MGMT (human) (recombinant)
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SYNONYMS O6-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-065-R050   50 µl 320.00 USD Add To Cart
Product Specification
EC: 2.1.1.63
SOURCE/HOST: Produced in E. coli. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY: Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION: Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY: 100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O6-methylguanine from DNA.
QUALITY CONTROL: The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P16455: MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-073 Revised 20-Nov-08
KillerTRAIL™, Soluble (human) (recombinant)
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SYNONYMS Apo-2L, Soluble (human) (recombinant)
TNFSF 10, Soluble (human) (recombinant)
CD253, Soluble (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY TRAIL [Apo-2L]
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ALX-201-073-C020   20 µg 360.00 USD Add To Cart
ALX-201-073-3020 SuperPack 3x20 µg 720.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.
CONCENTRATION: 0.5mg/ml   
PURITY: ≥99% (SDS-PAGE)
PURITY DETAIL: Affinity purified. Sterile filtered.
FORMULATION: Liquid. In 20mM HEPES, pH 7.9, 120mM sodium chloride, 0.01% Tween 20, 1% sucrose and 1mM DTT.
ENDOTOXIN CONTENT: ≤0.004EU/μg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005).
SPECIFICITY: Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).
BIOLOGICAL ACTIVITY: Induces apoptosis in a concentration range of 10-100ng/ml.
Note: Does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL™ (Prod. No. ALX-201-115).
SPECIFIC ACTIVITY: ED50: 20ng/ml (BJAB cells)
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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Product Specific Literature References
The cytokines tumor necrosis factor-α (TNF-α) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papilloma: J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001) Abstract; Full Text
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001) Abstract
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia 16, 67 (2002) Abstract; Full Text
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Sträter, et. al.; Gastroenterology 122, 659 (2002) Abstract
Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1: M. MacFarlane, et al.; Cell Death Differ. 12, 773 (2005) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P50591: TRAIL (human)
AfCS Signalling Gateway link A002862: TRAIL (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-077 Revised 10-Jul-07
DNase I-like Endonuclease 3 (human) (recombinant)
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SYNONYMS DNAS1L3 (human) (recombinant)
Deoxyribonuclease γ (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-201-077-C010   10 µg 350.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM potassium chloride and 50% glycerol.
APPLICATION: For studying DNAS1L3 catalyzed oligonucleosomal DNA fragmentation.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Specific Literature References
Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments: A.G. Yakovlev, et al.; Nucl. Acids Res. 27, 1999 (1999) Abstract
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase: A.G. Yakovlev, et al.; J. Biol. Chem. 275, 21302 (2000) Abstract; Full Text
Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation: A.H. Boulares, et al.; J. Biol. Chem. 277, 372 (2002) Abstract; Full Text
The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells: A.H. Boulares, et al.; Cancer Res. 62, 4439 (2002) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION For maximum activity, use at pH 7.5 with 2.5mM Ca2+ and 5mM Mg2+. Causes single strand nicks in duplex DNA and can be inhibited by Zn2+, but not by actin.
 
 
ALX-201-078 Revised 24-Jan-05
Caspase-3 (rat) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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ALX-201-078-C005   5 µg 320.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.165mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. 5µg of caspase-3 in 50mM HEPES, pH 7.4, 100mM NaCI, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 50% glycerol.
SPECIFIC ACTIVITY: ≥500U/µg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase-substrate Z-DEVD-AMC per hour at 20°C in a reaction solution containing 20µM substrate, 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 10% glycerol.
APPLICATION: In combination with caspase-3 activity assays, this product is useful in biological screening of caspase substrates and inhibitors. It can also be used as a positive control in caspase-3 assays as well as a positive control  in Western blot.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stability at -20°C: 1-2 weeks
HANDLING: Avoid freeze/thaw cycles.
Product Description
Contains both the proenzyme and the processed (active) form.
Product Specific Literature References
Presence of DNA fragmentation and lack of neuroprotective effect in DFF45 knockout mice subjected to traumatic brain injury: A.G. Yakovlev, et al.; Mol. Med. 7, 205 (2001) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Enzyme Evaluation Protocol (general)
This protocol can be used to measure caspase enzyme activity. A synthetic fluorogenic peptide, e. g. Ac-DEVD-AMC (Prod. No. ALX-260-031) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC. AMC release can be measured by spectrofluorometry using UV.
1. Add 20µM of Ac-DEVD-AMC to 1 ml of assay buffer (20mM PIPES, pH 7.2, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose). Add the appropriate amount of active caspase to the mixture (e.g. 50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AMC liberated from the Ac-DEVD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm.

Swiss-Prot link P55213: Caspase-3 (rat)
AfCS Signalling Gateway link A000498: Caspase-3 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-082 Revised 07-Feb-06
Procaspase-3 (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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ALX-201-082-C005   5 µg 413.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Requires activation.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 0.1mg/ml with PBS containing 15% glycerol.
APPLICATION: Very useful in studying mechanisms of caspase activation and regulation, as well as in investigating cascades of caspase-3 mediated signal transduction pathways.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C
General Information
Procaspase-3 (human) (recombinant) is highly activatable by treatment with active human recombinant caspase-8  (Prod. No. ALX-201-062). Activation is routinely tested using 10-30ng of procaspase-3 treated with 0.-0.5 units of the active recombinant caspase-8 in 100µl of reaction buffer for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric (Prod. No. ALX-850-216) and Colorimetric Assay Kits (Prod. No. ALX-850-215).
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-084 Revised 09-Feb-05
NEDD8 Precursor (human) (recombinant)
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PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY NEDD8
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ALX-201-084-M001   1 mg 238.00 USD Add To Cart
Product Specification
MW: ~9.1kDa.
PURITY: ≥96% (SDS-PAGE)
FORMULATION: Liquid.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Typical concentration for conjugate formation is 10-50µM depending on conditions.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-085 Revised 17-Jan-08
NEDD8 (human) (recombinant)
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PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY NEDD8
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ALX-201-085-C500   500 µg 216.00 USD Add To Cart
Product Specification
MW: ~9kDa
SOURCE/HOST: Recombinant human NEDD8 isolated from HeLa cells.
CONCENTRATION: 2.25mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM HEPES, pH 8.0, containing 100mM sodium chloride, 0.5mM EDTA and 1mM DTT.
APPLICATION: Typical in vitro concentration for conjugate formation is 10-50µM depending on conditions.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination: V.N. Podust, et al.; PNAS 97, 4579 (2000) Abstract
Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha: M.A. Read, et al.; Mol. Cell. Biol. 20, 2326 (2000) Abstract
Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity: D.P. Xirodimas, et al.; Cell 118, 83 (2004)
General Information
The ubiquitin-like protein NEDD8 is conjugated to proteins by NEDD8 E1 and Ubc12. NEDD8 conjugation is implicated in the turnover regulation of p27 (Kip 1) and is known to modify Cdc 53 and cullin family members of ubiquitin-protein ligases.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-086 Revised 23-Mar-06
SUMO-2 (1-103) (human) (recombinant)
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SYNONYMS Sentrin-3 (1-103) (human) (recombinant)
pro-SUMO-2 (human) (recombinant)
pro-Sentrin-3 (human) (recombinant)
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY SUMOs / Related Products
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ALX-201-086-C250   250 µg 270.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
APPEARANCE: Lyophilized.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Inactive precursor of human SUMO-2. Substrate for the characterization of SUMO-2 specific proteases.
Product Specific Literature References
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997) Abstract; Full Text
Protein modification by SUMO: R.T. Hay; TIBS 26, 332 Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-087 Revised 10-Feb-05
SUMO-2 (1-92) (human) (recombinant)
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SYNONYMS Sentrin-3 (1-92) (human) (recombinant)
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY SUMOs / Related Products
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ALX-201-087-C500   500 µg 360.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
APPEARANCE: Lyophilized powder.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Active human SUMO-2 for conjugation to protein substrates.
Product Specific Literature References
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997) Abstract; Full Text
Protein modification by SUMO: R.T. Hay; TIBS 26, 332 (2001) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-088 Revised 10-Feb-05
SUMO-3 (1-95) (human) (recombinant)
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SYNONYMS Sentrin-2 (1-95) (human) (recombinant)
pro-SUMO-3 (human) (recombinant)
pro-Sentrin-2 (human) (recombinant)
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY SUMOs / Related Products
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ALX-201-088-C250   250 µg 270.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
APPEARANCE: Lyophilized powder.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Inactive precursor of human SUMO-3. Substrate for the characterization of SUMO-3 specific proteases.
Product Specific Literature References
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997) Abstract; Full Text
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P55855: Sentrin 2 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-089 Revised 10-Feb-05
SUMO-3 (1-93) (human) (recombinant)
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SYNONYMS Sentrin-2 (1-93) (human) (recombinant)
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY SUMOs / Related Products
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ALX-201-089-C500   500 µg 360.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95%
FORMULATION: Lyophilized powder.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for a few days at room temperature.