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Glucocorticoids & Glucocorticoid Receptors / Related Products
You are here: Product Lines > DNA Regulation / Transcription > Nuclear Receptors / Related Products > Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-550-516 Revised 21-May-08
Compound A
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SYNONYMS CpdA
2-((4-Acetophenyl)-2-chloro-N-methyl)ethylammonium chloride
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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ALX-550-516-M005   5 mg 40.00 USD Add To Cart
ALX-550-516-M025   25 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C11H14ClNO2 . HCl
MW: 227.7 . 36.5
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or water.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stock solutions in ethanol are stable for up to 1 month when stored at -80°C.
HANDLING: Protect from moisture. After reconstitution, prepare aliquots and store at -80°C.

Product Description
Cell permeable, nonsteroidal glucocorticoid receptor modulator with high binding affinity. Exerts anti-inflammatory potential by down-modulating TNF-induced pro-inflammatory gene expression, such as IL-6 and E-selectin. Unlike dexamethasone (Prod. No. ALX-370-002) it does not enhance glucocorticoid response element (GRE)-driven gene expression or induce glucocorticoid receptor (GR) binding to GRE-dependent genes in vivo.
Product Specific Literature References
A fully dissociated compound of plant origin for inflammatory gene repression: K. De Bosscher, et al.; PNAS 102, 15827 (2005) Abstract
A plant-derived ligand favoring monomeric glucocorticoid receptor conformation with impaired transactivation potential attenuates collagen-induced arthritis: P. Dewint, et al.; J. Immunol. 180, 2608 (2008) Abstract
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Further Categories Containing This Product:
GITR & GITRL Other Products • Glucocorticoids & Glucocorticoid Receptors / Related Products • RANK & RANKL / OPG Other Products • DNA & RNA Methyl & Alkyl Transferases / Related Products • Antitumor Agents (Hormone-related) • NOS Inhibitors (NOS Induction & Enzyme Activity) • Natural Products - Chemopreventive Agents • Natural Products - Nitric Oxide Pathway Modulators • Other Natural Products - DNA Regulation / Transcription • Natural Products for Cell Cycle Research
 
 
ALX-370-007 Revised 09-Sep-08
Corticosterone
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SYNONYMS (11β)-11,21-Dihydroxypregn-4-ene-3,20-dione
Reichstein's Substance H
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-370-007-M050   50 mg 18.00 USD Add To Cart
Product Specification
FORMULA: C21H30O4
MW: 346.5
CAS NUMBER: 50-22-6
MERCK INDEX: 14: 2538
RTECS: GM7650000
PURITY: ≥98% (HPLC)
APPEARANCE: White to light yellow solid.
SOLUBILITY: Soluble in 100% ethanol (50mg/ml) or dioxane (20mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: MAY BE TERATOGENIC. IRRITANT.

Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products • Nitric Oxide Pathway Other Products
 
 
ALX-370-002 Revised 19-Sep-06
Dexamethasone
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SYNONYMS 9α-Fluoro-16α-methylprednisolone
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-370-002-M250   250 mg 22.00 USD Add To Cart
ALX-370-002-G001   1 g 45.00 USD Add To Cart
Product Specification
FORMULA: C22H29FO5
MW: 392.5
CAS NUMBER: 50-02-2
MERCK INDEX: 14: 2943
RTECS: TU3980000
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or methanol. Slightly soluble in acetone or chloroform. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
HAZARD: HARMFUL. MAY BE TERATOGENIC.

Product Description
Inhibits constitutive OPG mRNA steady-state levels in a dose- and time-dependent fashion, promoting osteoclast formation. Glucocorticoid with anti-inflammatory activity. Inhibits the induction of nitric oxide synthases (NOS).
Product Specific Literature References
Inhibition of the induction of nitric oxide synthase by glucocorticoids: yet another explanation for their anti-inflammatory effects?: S. Moncada & R.M.J. Palmer; TIPS 12, 130 (1991), (Review) Abstract
Glucocorticoids inhibit the induction of nitric oxide synthase and the related cell damage in adenocarcinoma cells: K.J. O'Connor & S. Moncada; Biochim. Biophys. Acta 1097, 227 (1991) Abstract
Nitric oxide mediates tumor necrosis factor-alpha cytotoxicity in endothelial cells: C. Estrada, et al.; BBRC 186, 475 (1992) Abstract
Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium: R.R. Dudek, et al.; BBRC 202, 1120 (1994) Abstract
Induction of the alkyltransferase (MGMT) gene by DNA damaging agents and the glucocorticoid dexamethasone and comparison with the response of base excision repair genes: T. Grombacher, et al.; Carcinogenesis 17, 2329 (1996) Abstract
Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone: T. Biswas, et al.; Oncogene 18, 525 (1999) Abstract
Dexamethasone-induced thymocyte apoptosis: apoptotic signal involves the sequential activation of phosphoinositide-specific phospholipase C, acidic sphingomyelinase, and caspases: M.G. Cifone, et al.; Blood 93, 2282 (1999) Abstract; Full Text
Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2: M.S. Montani, et al.; Cell Death Differ 6, 79 (1999) Abstract
Dexamethasone inhibits the phosphorylation of retinoblastoma protein in the suppression of human vascular smooth muscle cell proliferation: T.D. Reil, et al.; J. Surg. Res. 92, 108 (2000) Abstract
Dexamethasone suppresses iNOS gene expression by inhibiting NF-kappaB in vascular smooth muscle cells: M. Matsumura, et al.; Life Sci. 69, 1067 (2001) Abstract
Dexamethasone inhibits inducible nitric-oxide synthase expression and nitric oxide production by destabilizing mRNA in lipopolysaccharide-treated macrophages: R. Korhonen, et al.; Mol. Pharmacol. 62, 698 (2002) Abstract; Full Text
Molecular mechanisms underlying dexamethasone inhibition of iNOS expression and activity in C6 glioma cells: J. Shinoda, et al.; Glia 42, 68 (2003) Abstract
Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-kappaB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line: A.L. Vital, et al.; Mediators Inflamm. 12, 71 (2003) Abstract
Dexamethasone suppresses eNOS and CAT-1 and induces oxidative stress in mouse resistance arterioles: S.C. Schafer, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H436 (2005) Abstract; Full Text
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Further Categories Containing This Product:
GITR & GITRL Other Products • RANK & RANKL / OPG Other Products • Antitumor Agents (Hormone-related) • NOS Inhibitors (NOS Induction & Enzyme Activity) • Chemopreventive Agents Other Products • DNA & RNA Methyl & Alkyl Transferases / Related Products • Cell Cycle Other Products
 
 
ALX-850-021 Revised 17-Sep-07
Glucocorticoid Receptor Discovery Kit
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-850-021-KI01   1 Set 367.00 USD Add To Cart
Product Specification
QUANTITY: 50 assays.
KIT/SET CONTAINS: Recombinant human glucocorticoid receptor (full length) expressed in insect cells and enriched by ammonium sulfate precipitation, followed by dialysis in KED buffer with 20% glycerol.
Oligonucleotide double stranded DNA corresponding to the human tyrosine amino transferase gene response element:
5’...CTA GGC TGT ACA GGA TGT TCT GCC TAG...3’
3’...GAT CCG ACA TGT CCT ACA AGA CGG ATC...5’
APPLICATION: Components of this kit may be used to perform gel shift assays or as a control for Western blot detection with anti-human glucocorticoid receptor antibodies (Prod. No. ALX-210-155, ALX-210-131 or ALX-210-153).
Complete system can be used as a control system into which the researcher may introduce other components such as other putative response elements or other glucocorticoid receptors (mutants, from various tissue sources, etc.).
Recombinant protein may also be used to perform DNase I footprinting experiments or as a control in Western blot experiments or for in vitro transcription assays.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-270-474 Revised 22-Oct-07
HSD Inhibitor 23
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SYNONYMS N-(5-Hydroxytricyclo[3.3.1.13,7]dec-2-yl)-α,α-dimethyl-4-[5-(trifluoromethyl)-2-pyridinyl]-1-piperazineacetamide
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-270-474-M001   1 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C24H33F3N4O2
MW: 466.5
CAS NUMBER: 868604-75-5
PURITY: ≥95%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
IDENTITY: Determined by 1H-NMR, 13C-NMR and MS.

Product Description
Selective, potent and cell permeable small molecule inhibitor of 11-β-hydroxysteroid dehydrogenase.
Product Specific Literature References
Discovery and metabolic stabilization of potent and selective 2-amino-N-(adamant-2-yl) acetamide 11beta-hydroxysteroid dehydrogenase type 1 inhibitors: J.J. Rohde, et al.; J. Med. Chem. 50, 149 (2007) Abstract; Full Text
 
 
ALX-803-006 Revised 11-Jun-07
Monoclonal Antibody to Glucocorticoid Receptor (BuGR2)
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SYNONYMS anti-GR MAb (BuGR2)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-803-006-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Sheep
Others
CLONE: BuGR2
ISOTYPE: Mouse IgG2
FORMULATION: Lyophilized from PBS, pH 7.2, containing 0.05% sodium azide.
RECONSTITUTION: Reconstitute with distilled water.
IMMUNOGEN: Partially purified rat GR (glucocorticoid receptor).
SPECIFICITY: Recognizes mouse, rat, sheep, guinea pig and rabbit GR. Recognizes full-lengh GR, the 17kDa DNA binding trypsin fragment and the 45kDa steroid- and DNA-binding chymotrypsin fragment. Detects a band of ~97kDa by Western blot. Does not cross-react with primate, avian or amphibian GR.
APPLICATION: Flow Cytometry
Gel Shift Assay
Immunocytochemistry
Immunohistochemistry (paraffin sections (5µg/ml))
Immunoprecipitation
Western Blot (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Analysis of the disruptive action of an epididymal protease and the stabilizing influence of molybdate on nondenatured and denatured glucocorticoid receptor: W.J. Hendry, 3rd, et al.; Endocrinology 120, 629 (1987) Abstract
Phosphorylated sites within the functional domains of the approximately 100-kDa steroid-binding subunit of glucocorticoid receptors: L.I. Smith, et al.; Biochemistry 28, 4490 (1989) Abstract
Disruption of the glucocorticoid receptor assembly with heat shock protein 90 by a peptidic antiglucocorticoid: H.P. Dao-Phan, et al.; Mol. Endocrinol. 11, 962 (1997) Abstract; Full Text
Analysis of FKBP51/FKBP52 chimeras and mutants for Hsp90 binding and association with progesterone receptor complexes: R.L. Barent, et al.; Mol. Endocrinol. 12, 342 (1998) Abstract; Full Text
Glucocorticoid receptor/signal transducer and activator of transcription 5 (STAT5) interactions enhance STAT5 activation by prolonging STAT5 DNA binding and tyrosine phosphorylation: S.L. Wyszomierski, et al.; Mol. Endocrinol. 13, 330 (1999) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Immunocytochemical staining of GR in L929 cells with Prod. No. ALX-803-006 results in staining of both the cytoplasm and nucleus, even in the presence of hormone.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-131 Revised 19-Nov-07
Polyclonal Antibody to Glucocorticoid Receptor
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SYNONYMS anti-GR PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-210-131-C050   50 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 346-367 (D346QKPIFNVIPPIPVGSENWNRC367) of human GR (glucocorticoid receptor).
SPECIFICITY: Recognizes all forms of human, mouse and rat GR. Detects a band of ~97kDa by Western blot. Does not interfere with receptor DNA binding.
APPLICATION: Immunocytochemistry (1µg/ml)
Immunohistochemistry (paraffin sections)
Western Blot (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-157-015.
Product Specific Literature References
Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors: J.A. Cidlowski, et al.; Mol. Endocrinol. 4, 1427 (1990) Abstract
Liganded androgen receptor interaction with beta-catenin: nuclear co-localization and modulation of transcriptional activity in neuronal cells: J.E. Pawlowski, et al.; J. Biol. Chem. 277, 20702 (2002) Abstract; Full Text
Presence of a transcriptionally active glucocorticoid receptor alpha in lens epithelial cells: E.R. James, et al.; Invest. Ophthalmol. Vis. Sci. 44, 5269 (2003) Abstract; Full Text
Differential effects of adrenalectomy on melanin-concentrating hormone and orexin A: D.L. Drazen, et al.; Endocrinology 145, 3404 (2004) Abstract
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-153 Revised 07-May-07
Polyclonal Antibody to Glucocorticoid Receptor
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SYNONYMS anti-GR PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-210-153-C050   50 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 245-259 (C245KPLILPDTKPKIKD259) of human GR (glucocorticoid receptor).
SPECIFICITY: Recognizes human, mouse and rat GR. Detects a band of ~97kDa by Western blot. Does not interfere with receptor DNA binding.
APPLICATION: Gel Shift Assays
Immunohistochemistry (frozen sections (1µg/ml))
Immunoprecipitation (5µg/m), Chromatin Immunoprecipitation
Western Blot (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-157-016.
Product Specific Literature References
Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple forms in vitro and distinct localization of cytoplasmic and nuclear receptors: J.A. Cidlowski, et al.; Mol. Endocrinol. 4, 1427 (1990) Abstract
Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure: C.L. Smith, et al.; J. Biol. Chem. 272, 14227 (1997) Abstract; Full Text
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-155 Revised 11-Nov-05
Polyclonal Antibody to Glucocorticoid Receptor
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SYNONYMS anti-GR PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-210-155-R200   200 µl 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 150-175 (A150PIEKEIPKIHSDVSSEQQHLKGQTG175) of human GR (glucocorticoid receptor).
SPECIFICITY: Recognizes human and rat GR. Detects a band of ~97kDa by Western blot.
APPLICATION: Gel Supershift Assay
Immunocytochemistry (1:100)
Immunoprecipitation
Western Blot (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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General Information
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-277 Revised 14-Feb-07
Polyclonal Antibody to Glucocorticoid Receptor α
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SYNONYMS anti-GRα PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-210-277-C050   50 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Lyophilized. Contains 0.05% sodium azide.
RECONSTITUTION: Reconstitute with PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 755-771 (C755EIITNQIPKYSN
GNIKK771) of human GRα (glucocorticoid receptor α).
SPECIFICITY: Recognizes human, mouse and rat GRα. Detects a band of ~94kDa by Western blot.
APPLICATION: