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ALX-850-299 Revised 18-Aug-08
PTX3 (human) Detection Set [For ELISA Application]
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3]/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-299-KI01   1 Set 995.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: Detection limit: 75pg/ml.
KIT/SET CONTAINS: Contains
- 1 vial (10µg) PTX3 (human) (rec.) (Prod. No. ALX-201-124)
- 1 vial (50µg) PAb to PTX3 (Biotin) [Detection Antibody] (Prod. No. ALX-210-365B)
- 1 vial (100µg) MAb to PTX3 (human) (MNB4) [Capture Antibody]  (Prod. No. ALX-804-464)
APPLICATION: For the quantitative determination of PTX3 in human and bovine (weak) cell culture media, EDTA-plasma or serum (weak) samples.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Once thawed store biotinylated antibody at 4°C and do not re-freeze.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNFalpha, LPS, and cyclic stretch: C.C. dos Santos, et al.; Physiol. Genomics 19, 331 (2004) Abstract; Full Text
TNFalpha-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK: B. Han, et al.; J. Immunol. 175, 8303 (2005) Abstract; Full Text
The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells: P. Baruah, et al.; J. Leukoc. Biol. 80, 87 (2006) Abstract; Full Text
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract
Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris: K. Inoue, et al.; Arterioscler. Thromb. Vasc. Biol. 27, 161 (2007) Abstract; Full Text
Calorie restriction modulates inactivity-induced changes in the inflammatory markers C-reactive protein and pentraxin-3: A. Bosutti, et al.; J. Clin. Endocrinol. Metab. 93, 3226 (2008) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Sandwich ELISA for PTX3

- 96 well-ELISA  plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 min. at 405nm in an automatic ELISA reader.

Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.
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ELISA Sets
 
 
ALX-850-302 Revised 16-Nov-05
CD14, Soluble (human) Detection Set [For ELISA Application]
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PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
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ALX-850-302-KI01   1 Set 690.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble human CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
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ELISA Sets
 
 
ALX-850-303 Revised 16-Nov-05
CD14, Soluble (mouse) Detection Set [For ELISA Application]
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PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-303-KI01   1 Set 990.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble mouse CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
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ELISA Sets
 
 
ALX-850-304 Revised 06-Nov-06
LBP, Soluble Detection Set [For ELISA Application]
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SYNONYMS Lipopolysaccharide Binding Protein, Soluble Detection Set [For ELISA Application]
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharide Binding Protein [LBP]/Related Products
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ALX-850-304-KI01   1 Set 690.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Dog
Pig
QUANTITY: 96 wells (~80 tests)
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of human, dog, pig, rabbit, bovine and horse soluble LBP levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
ALX-850-305 Revised 06-Nov-06
LBP, Soluble (mouse) Detection Set  [For ELISA Application]
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SYNONYMS Lipopolysaccharide Binding Protein, Soluble (mouse) Detection Set [For ELISA Application]
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharide Binding Protein [LBP]/Related Products
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ALX-850-305-KI01   1 Set 690.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests)
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of mouse soluble LBP levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
APO-54N-013 Revised 20-Jun-08
BAFF, Soluble (mouse) Detection Set [For ELISA Application]
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SYNONYMS BLyS, Soluble (mouse) Detection Set [For ELISA Application]
TALL-1, Soluble (mouse) Detection Set [For ELISA Application]
zTNF4, Soluble (mouse) Detection Set [For ELISA Application]
TNFSF 13B/20, Soluble (mouse) Detection Set [For ELISA Application]
CD257, Soluble (mouse) Detection Set [For ELISA Application]
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
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APO-54N-013-KI01   1 Set 1'050.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.3ng/ml (range 0 to 20ng/ml)
QUANTITY: For 4x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of soluble mouse BAFF from biological fluids (serum and cell culture supernatant).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Toll-like Receptor 9-Dependent and -Independent Dendritic Cell Activation by Chromatin-Immunoglobulin G Complexes: M.W. Boule, et al.; J. Exp. Med. 199, 1631 (2004) Abstract
Regulation of B-cell survival by BAFF-dependent PKCdelta-mediated nuclear signalling: I. Mecklenbrauker, et al.; Nature 431, 456 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
A role for B cell-activating factor of the TNF family in chemically induced autoimmunity: Y. Zheng, et al.; J. Immunol. 175, 6163 (2005) Abstract
Development of autoantibody responses in NC/Nga mice: its prevention by pulverized konjac glucomannan feeding: N. Onishi, et al.; Arch. Dermatol. Res. 300, 95 (2008) Abstract
General Information
BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link : BAFF (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The TNF family members BAFF and APRIL: the growing complexity: F. Mackay & C. Ambrose; Cytokine Growth Factor Rev. 14, 311 (2003) Abstract
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
The BAFF/APRIL system: an important player in systemic rheumatic diseases: F. Mackay, et al.; Curr. Dir. Autoimmun. 8, 243 (2005) Abstract
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ELISA Sets
 
 
APO-54N-017 Revised 24-Jun-08
LAG-3, Soluble (human) Detection Set [For ELISA Application]
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SYNONYMS Lymphocyte Activation Gene-3, Soluble (human) Detection Set
FDC Protein, Soluble (human) Detection Set
CD223, Soluble (human) Detection Set
PRODUCT LINE Immunology
PRODUCT CATEGORY Dendritic Cells [DC]
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APO-54N-017-KI01   1 Set 950.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.05ng /ml (range 0 to 4ng/ml)
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of soluble human LAG-3 from biological fluids (serum and cell culture supernatant).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C.
Product Specific Literature References
Demonstration of antiglobulin activity in the synovial membrane of patients with rheumatoid arthritis and ankylosing spondylitis after pepsin treatment: real or artefact?: P.G. Davies, et al.; Ann. Rheum. Dis. 45, 821 (1986) Abstract
Preferential Th1 profile of T helper cell responses in X-linked (Bruton's) agammaglobulinemia: A. Amedei, et al.; Eur. J. Immunol. 31, 1927 (2001) Abstract
Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation: C. Casati, et al.; J. Immunol. 180, 3782 (2008) Abstract
General Information
The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4 [1]. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa) [1,2]. The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome [3]. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses [4].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P18627: LAG-3 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination: F. Triebel; Trends Immunol. 24, 619 (2003) Abstract
[2] A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006) Abstract
[3] Active tuberculosis in Africa is associated with reduced Th1 and increased Th2 activity in vivo: C. Lienhardt, et al.; Eur. J. Immunol. 32, 1605 (2002) Abstract
[4] Expression and release of LAG-3-encoded protein by human CD4+ T cells are associated with IFN-gamma production: F. Annunziato, et al.; FASEB J. 10, 769 (1996) Abstract
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Further Categories Containing This Product:
CD Kits & Sets • ELISA Sets
 
 
APO-54N-022 Revised 01-Apr-08
BAFF, Soluble (human) Detection Set [For ELISA Application]
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SYNONYMS BLyS, Soluble (human) Detection Set [For ELISA Application]
TALL-1, Soluble (human) Detection Set [For ELISA Application]
zTNF4, Soluble (human) Detection Set [For ELISA Application]
TNFSF 13B/20, Soluble (human) Detection Set [For ELISA Application]
CD257, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-022-KI01   1 Set 560.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.3ng/ml (range 0 to 30ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD)
1 vial Coating Antibody (COAT)
1 vial Detection Antibody (DET)
1 vial Goat anti-rat IgG-HRP (HRP)
APPLICATION: For the quantitative determination of soluble human BAFF from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse BAFF.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases. 
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9Y275: BAFF (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The TNF family members BAFF and APRIL: the growing complexity: F. Mackay & C. Ambrose; Cytokine Growth Factor Rev. 14, 311 (2003) Abstract
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
The BAFF/APRIL system: life beyond B lymphocytes: L.G. Ng, et al.; Mol. Immunol. 42, 763 (2005) Abstract
The BAFF/APRIL system: an important player in systemic rheumatic diseases: F. Mackay, et al.; Curr. Dir. Autoimmun. 8, 243 (2005) Abstract
Further Categories Containing This Product:
ELISA Sets
 
 
APO-54N-023 Revised 22-Oct-07
CTRP7 Detection Set [For ELISA Application]
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SYNONYMS C1q Tumor Necrosis Factor-α-related Protein 7 Detection Set [For ELISA Application]
C1qTNF7 Detection Set [For ELISA Application]
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY C1q Tumor Necrosis Factor-alpha-related Proteins [CTRPs]
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APO-54N-023-KI01   1 Set 560.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SENSITIVITY: 0.1ng/ml (range 0 to 10ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human and mouse CTRP7 from biological fluids (serum and cell culture supernatant).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
Adipose tissue functions as storage of energy and plays an active role in monitoring and controlling whole-body metabolism by secreting a number of proinflammatory adipokines, (such as leptin, resistin, tumor necrosis factor-α and interleukin-6), as well as one anti-inflammatory adipokine, adiponectin. Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle [1]. Recently, a new highly conserved family of adiponectin paralogs designated as C1q/Tumor Necrosis Factor-α-Related Proteins (CTRPs) 1-7, has been described in mouse and human [2-5]. This family of proteins exhibits similar structural and biological properties to adiponectin. The CTRPs, unlike adiponectin whose expression is restricted to adipose tissue, are widely expressed by many different adult tissues. Like adiponectin, CTRP2 rapidly induces acetyl-CoA carboxylase, and mitogen-activated protein kinase phosphorylation in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation, and CTRP7 (as well as CTRPs 1, 2 and 3) induces AMP-activated protein kinase phosphorylation. The activity of the CTRPs suggests that some members of the CTRP family of proteins could substitute for adiponectin function in vivo.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q8BVD7: CTRP7 (mouse)
Swiss-Prot link Q9BXJ2: CTRP7 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Adipose tissue, adipokines, and inflammation: G. Fantuzzi; J. Allergy Clin. Immunol. 115, 911 (2005) Abstract
[2] A family of Acrp30/adiponectin structural and functional paralogs: G.W. Wong, et al.; PNAS 101, 10302 (2004) Abstract
[3] C1qTNF-related protein-1 (CTRP-1): a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen: G. Lasser, et al.; Blood 107, 423 (2006) Abstract
[4] Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes: T. Maeda, et al.; J. Cell Physiol. 206, 537 (2006) Abstract
[5] The adiponectin paralog CORS-26 has anti-inflammatory properties and is produced by human monocytic cells: J. Weigert, et al.; FEBS Lett. 579, 5565 (2005) Abstract
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Further Categories Containing This Product:
ELISA Sets
 
 
APO-54N-024 Revised 22-Oct-07
TL1A, Soluble (human) Detection Set [For ELISA Application]
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SYNONYMS TNFSF 15, Soluble (human) Detection Set [For ELISA Application]
VEGI, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY HVEM & LIGHT/LT-betaR/DcR3/Related Products
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APO-54N-024-KI01   1 Set 540.00 USD Add To Cart