ALEXIS Biochemicals: Polyclonal Antibody to TIGAR (AT122)

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ALX-270-022

Revised 09-Jan-08

AEBSF . hydrochloride
SYNONYMS	4-(2-Aminoethyl)benzenesulfonylfluoride . HCl Pefabloc^®SC
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Proteases Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-022-M050		50 mg	40.00 USD
ALX-270-022-M250		250 mg	145.00 USD
ALX-270-022-G001		1 g	360.00 USD

Product Specification

FORMULA:	C₈H₁₀FNO₂S . HCl
MW:	203.2 . 36.5
CAS NUMBER:	30827-99-7
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from moisture.

Product Description

Specific irreversible inhibitor of serine proteases including trypsin, chymotrypsin, plasmin, plasma kallikrein, and thrombin. Stable, non-toxic alternative to PMSF (Prod. No. ALX-270-184). Inhibitor of NAD(P)H oxidase. Activator of MAP kinases JNK and p38.

Product Specific Literature References

Irreversible enzyme inhibitors. 180. Irreversible inhibitors of the C'la component of complement derived from m-(phenoxypropoxy)benzamidine and phenoxyacetamide: B.R. Baker & H.J. Cory; J. Med. Chem. 14, 119 (1971) Abstract
Serine protease inhibitors block priming of monocytes for enhanced release of superoxide: P. Megyeri, et al.; Immunology 86, 629 (1995) Abstract; Full Text
Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF: M. Citron, et al.; Neuron 17, 171 (1996) Abstract
Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor: Y. Nakabo & M.J. Pabst; J. Leukoc. Biol. 60, 328 (1996) Abstract; Full Text
Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds: V. Diatchuk, et al.; J. Biol. Chem. 272, 13292 (1997) Abstract; Full Text
4-(2-Aminoethyl)benzenesulfonyl fluoride attenuates tumor-necrosis-factor-alpha-induced blood-brain barrier opening: P. Megyeri, et al.; Eur. J. Pharmacol. 374, 207 (1999) Abstract
Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis: R. Buitrago-Rey, et al.; J. Antimicrob. Chemother. 49, 871 (2002) Abstract; Full Text
Heme oxygenase-1 gene activation by the NAD(P)H oxidase inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride via a protein kinase B, p38-dependent signaling pathway in monocytes: N. Wijayanti, et al.; J. Biol. Chem. 280, 21820 (2005) Abstract; Full Text
Inhibitory effect of 4-(2-aminoethyl)-benzenesulfonyl fluoride, a serine protease inhibitor, on PI3K inhibitor-induced CHOP expression: T. Hosoi, et al.; Eur. J. Pharmacol. 554, 8 (2007) Abstract

Further Categories Containing This Product:

Heme Oxygenase/Related Products • Cell Stress, Oxidative Stress & Redox Signalling Other Products • MAPK Pathway Activators • Fatty Acid Amide Hydrolase [FAAH]/Related Products

ALX-430-137

Revised 17-Apr-07

IM-54
SYNONYMS	2-(1H-Indol-3-yl)-3-pentylamino-maleimide
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Non-apoptotic Cell Death/Necrosis

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-137-M001		1 mg	30.00 USD
ALX-430-137-M025		25 mg	420.00 USD

Product Specification

FORMULA:	C₁₉H₂₃N₃O₂
MW:	325.4
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow powder.
SOLUBILITY:	Soluble in DMSO (100mg/ml), dichloomethane, 100% ethanol or methanol (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Cell permeable inhibitor of necrosis. Selectively blocks oxidative stress-induced necrotic cell death (~3µM IM-54 prevented ~50% cell death in HL60 cells exposed to 100µM H₂O₂). Does not protect etoposide-induced apoptosis or display antioxidant properties. Does not affect S6K1 and PKC kinase activities at concentrations of 50µM.

Product Specific Literature References

Inhibition of hydrogen peroxide-induced necrotic cell death with 3-amino-2-indolylmaleimide derivatives: K. Dodo, et al.; Bioorg. Med. Chem. Lett. 15, 3114 (2005) Abstract

Further Categories Containing This Product:

Cell Stress, Oxidative Stress & Redox Signalling Other Products

ALX-460-007

Revised 02-Feb-07

Menadione
SYNONYMS	Vitamin K₃ 2-Methyl-1,4-naphthoquinone
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Vitamins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-460-007-G010		10 g	10.00 USD

Product Specification

FORMULA:	C₁₁H₈O₂
MW:	172.2
CAS NUMBER:	58-27-5
MERCK INDEX:	14: 5831
RTECS:	QL9100000
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow crystals.
SOLUBILITY:	Soluble in 96% ethanol (16mg/ml) or DMSO (1mg/ml); almost insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
HANDLING:	Protect from light.
HAZARD:	HARMFUL.

Product Description

Cytotoxic in various cell lines. Induces apoptosis via elevation of peroxide and superoxide radical levels. Affects gap-junctional intercellular communication by mediation of tyrosine phosphorylation.

Product Specific Literature References

Vitamin K3 induces cell cycle arrest and cell death by inhibiting Cdc25 phosphatase: F.Y. Wu and T.P.; Eur. J. Cancer 35, 1388 (1999) Abstract
Apoptosis-inducing activity of vitamin C and vitamin K: H. Sakagami, et al.; Cell Mol. Biol. 46, 129 (2000) Abstract
Cytotoxic effect through fas/APO-1 expression due to vitamin K in human glioma cells: L.K. Sun, et al.; J. Neurooncol. 47, 31 (2000) Abstract
Cytotoxic activity of vitamins K1, K2 and K3 against human oral tumor cell lines: H. Okayasu, et al.; Anticancer Res. 21, 2387 (2001) Abstract
2-Methyl-1,4-naphthoquinone, vitamin K(3), decreases gap-junctional intercellular communication via activation of the epidermal growth factor receptor/extracellular signal-regulated kinase cascade: L.O. Klotz, et al.; Cancer Res. 62, 4922 (2002) Abstract; Full Text
Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy: J. Mauzeroll, et al.; PNAS 101, 17582 (2004) Abstract; Full Text
Menadione-induced reactive oxygen species generation via redox cycling promotes apoptosis of murine pancreatic acinar cells: D.N. Criddle, et al.; J. Biol. Chem. 281, 40485 (2006) Abstract; Full Text

Further Categories Containing This Product:

Antitumor Agents (DNA Interaction & Gene Regulation) • Antitumor Agents (Apoptosis Inducers) • Cell Stress, Oxidative Stress & Redox Signalling Other Products

ALX-152-006

Revised 23-Jan-07

Bradykinin
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Bradykinin Receptor/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-152-006-M005		5 mg	22.00 USD
ALX-152-006-M025		25 mg	86.00 USD

Product Specification

SEQUENCE:	H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH
FORMULA:	C₅₀H₇₃N₁₅O₁₁
MW:	1060.3
CAS NUMBER:	5979-11-3
MERCK INDEX:	14: 1359
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in 5% acetic acid.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Activator of eNOS (NOS III).

Product Specific Literature References

Different activation of L-arginine pathway by bradykinin, serotonin, and clonidine in coronary arteries: V. Richard, et al.; Am. J. Physiol. 259, H1433 (1990) Abstract
Bradykinin and ATP stimulate L-arginine uptake and nitric oxide release in vascular endothelial cells: R.G. Bogle, et al.; BBRC 180, 926 (1991) Abstract
Activation of a small-conductance Ca(2+)-dependent K+ channel contributes to bradykinin-induced stimulation of nitric oxide synthesis in pig aortic endothelial cells: K. Groschner, et al.; Biochim. Biophys. Acta 1137, 162 (1992) Abstract
G proteins in aortic endothelial cells and bradykinin-induced formation of nitric oxide: J. Gil-Longo, et al.; Eur. J. Pharmacol. 247, 119 (1993) Abstract
Simultaneous measurements of Ca2+ and nitric oxide in bradykinin-stimulated vascular endothelial cells: L.A. Blatter, et al.; Circ. Res. 76, 922 (1995) Abstract; Full Text
Biosynthesis of endothelium-derived nitric oxide by bradykinin as endogenous precursor: A.R. Volpe, et al.; Immunopharmacology 33, 287 (1996) Abstract
Nitric oxide as mediator of bradykinin-induced pancreatic circulatory and metabolic responses: W.W. Pawlik, et al.; J. Physiol. Pharmacol. 48, 751 (1997) Abstract
Bradykinin-regulated interactions of the mitogen-activated protein kinase pathway with the endothelial nitric-oxide synthase: S.G. Bernier, et al.; J. Biol. Chem. 275, 30707 (2000) Abstract; Full Text
Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation: M.B. Harris, et al.; J. Biol. Chem. 276, 16587 (2001) Abstract; Full Text
Bradykinin down-regulates, whereas arginine analogs up-regulates, endothelial nitric-oxide synthase expression in coronary endothelial cells: N.D. Vaziri, et al.; J. Pharmacol. Exp. Ther. 313, 121 (2005) Abstract; Full Text
Bradykinin mediates phosphorylation of eNOS in odontoblasts: Y. Korkmaz, et al.; J. Dent. Res. 85, 536 (2006) Abstract

Further Categories Containing This Product:

NOS Regulation • Cell Stress, Oxidative Stress & Redox Signalling Other Products • Peptides

ALX-152-031

Revised 24-Nov-06

(Des-Arg1)-Bradykinin
SYNONYMS	Bradykinin (2-9)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Bradykinin Receptor/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-152-031-M001		1 mg	15.00 USD
ALX-152-031-M005		5 mg	50.00 USD

Product Specification

SEQUENCE:	H-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH
FORMULA:	C₄₄H₆₁N₁₁O₁₀
MW:	904.0
CAS NUMBER:	16875-11-9
APPEARANCE:	Lyophilized.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Further Categories Containing This Product:

NOS Regulation • Cell Stress, Oxidative Stress & Redox Signalling Other Products • Peptides

ALX-152-032

Revised 08-Dec-06

(Des-Arg9)-Bradykinin
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Bradykinin Receptor/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-152-032-M005		5 mg	75.00 USD
ALX-152-032-M025		25 mg	300.00 USD

Product Specification

SEQUENCE:	H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-OH
FORMULA:	C₄₄H₆₁N₁₁O₁₀
MW:	904.0
CAS NUMBER:	15958-92-6
PURITY:	≥99%
APPEARANCE:	White lyophilized powder.
SOLUBILITY:	Soluble in water (1mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Further Categories Containing This Product:

Cell Stress, Oxidative Stress & Redox Signalling Other Products • NOS Regulation • Peptides

ALX-803-301

Revised 31-Oct-06

Monoclonal Antibody to Melusin (human) (5E1)
SYNONYMS	anti-Integrin β1-binding Protein 2 (human) MAb (5E1) anti-ITGB1BP2 (human) MAb (5E1)
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Cell Stress, Oxidative Stress & Redox Signalling Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-301-C100		100 µg	240.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	5E1
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.2mg/ml
PURITY:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL:	Protein A-affinity purified.
IMMUNOGEN:	Recombinant human melusin (integrin β1-binding protein 2).
SPECIFICITY:	Recognizes human melusin. Cross-reacts weakly with mouse melusin.
APPLICATION:	ELISA Immunocytochemistry (5µg/ml) Immunohistochemistry (5µg/ml) Immunoprecipitation Western Blot (2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Further Categories Containing This Product:

GSK-3 Other Products • Monoclonal Antibodies

ALX-803-302

Revised 06-Aug-07

Monoclonal Antibody to CHP1 (5E9G5)
SYNONYMS	anti-CHORD Containing Protein-1 MAb (5E9G5) anti-CHORDC1 MAb (5E9G5)
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Cell Stress, Oxidative Stress & Redox Signalling Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-302-C100		100 µg	240.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
CLONE:	5E9G5
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.2mg/ml
PURITY:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL:	Protein A-affinity purified.
IMMUNOGEN:	Recombinant mouse CHP1 (CHORD containing protein-1).
SPECIFICITY:	Recognizes human and mouse CHP1.
APPLICATION:	ELISA Immunocytochemistry (5µg/ml) Immunohistochemistry (5µg/ml) Immunoprecipitation (5µg per 5x10⁶ cells) Western Blot (2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

General Literature References

Chp-1 and melusin, two CHORD containing proteins in vertebrates: M. Brancaccio, et al.; FEBS Lett. 551, 47 (2003) Abstract; Full Text

Further Categories Containing This Product:

Co-Chaperones & HSP-interacting Proteins/Related Products • Nod-complex/Related Products • Monoclonal Antibodies

ALX-804-647

Revised 13-Oct-06

Monoclonal Antibody to Bradykinin (MBK3)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Bradykinin Receptor/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-647-R100		100 µl	210.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
CLONE:	MBK3
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. Undiluted ascites.
IMMUNOGEN:	Bradykinin (H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH).
SPECIFICITY:	Recognizes bradykinin from various species.
APPLICATION:	Western Blot (1:1’000-1:4’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Anti-idiotypic antibodies bearing the internal image of a bradykinin epitope. Production, characterization, and interaction with the kinin receptor: M. Haasemann, et al.; J. Immunol. 147, 3882 (1991) Abstract

SPECIES CROSSREACTIVITY:	Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 644-659 (CN⁶⁴⁴YEFKKITED TVEFGS⁶⁵⁹) of C-terminal rat PMP70 (peroxisomal membrane protein 70). This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single aa substitution.
SPECIFICITY:	Recognizes mouse PMP70. Detects a band of ~70kDa by Western blot.
APPLICATION:	Flow Cytometry Western Blot (1:500)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-036.