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MPTP [Mitochondrial Transition Pore] / Related Products
You are here: Product Lines > Cell Death / Apoptosis / Autophagy > Mitochondrial Pathway > MPTP [Mitochondrial Transition Pore] / Related Products
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ALX-210-785/1 Revised 27-Mar-08
Polyclonal Antibody to Voltage-dependent Anion Channel
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SYNONYMS anti-VDAC PAb
anti-Mitochondrial Porin PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY MPTP [Mitochondrial Transition Pore] / Related Products
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ALX-210-785/1-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to a part (CNDGTEFGGSIYQK) of C-terminal human VDAC (voltage-dependent anion channel) with an added N-terminal cysteine. This sequence is completely conserved between isoforms 1, 2 and 3 of human, mouse and rat VDAC.
SPECIFICITY: Recognizes rat VDAC. Detects a band of ~31kDa by Western blot.
APPLICATION: Western Blot (2µg/ml)
Note: Not recommended for Immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-170-002.
Product Specific Literature References
Phospholipid scramblase 3 controls mitochondrial structure, function, and apoptotic response: J. Liu, et al.; Mol. Cancer Res. 1, 892 (2003) Abstract; Full Text
Proteome analysis of DNA damage-induced neuronal death using high throughput mass spectrometry: M.D. Johnson, et al.; J. Biol. Chem. 279, 26685 (2004) Abstract; Full Text
Modulation of mitochondrial transition pore components by thyroid hormone: E. Yehuda-Shnaidman, et al.; Endocrinology 146, 2462 (2005) Abstract; Full Text
Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria: M.R. Brown, et al.; J. Biol. Chem. 281, 11658 (2006) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Sequence information of human VDAC: Swiss Prot Accession Number P21796. http://www.expasy.ch/cgi-bin/niceprot.pl?P21796
General Literature References
The mitochondrial permeability transition pore and its role in cell death: M. Crompton; Biochem. J. 341 (Pt 2), 233 (1999), Review Abstract; Full Text
Further Categories Containing This Product:
Ion Channels & Transporters Other ProductsPolyclonal Antibodies
 
 
ALX-210-789 Revised 06-Aug-07
Polyclonal Antibody to Cyclophilin D
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SYNONYMS anti-CyPD PAb
anti-Peptidyl-prolyl cis-trans Isomerase D PAb
PRODUCT LINE Immunology
PRODUCT CATEGORY Immunophilins / Related Products
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ALX-210-789-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to N-terminal aa 42-54 (S42GNPLVYLDVDAN54C) of mature human cyclophilin D.
SPECIFICITY: Recognizes human, mouse, rat, hamster and dog cyclophilin D. Detects a band of ~15kDa by Western blot from rat liver and heart extract, and a ~37kDa band from rat brain extract.
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-033.
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Product Specific Literature References
Modulation of mitochondrial transition pore components by thyroid hormone: E. Yehuda-Shnaidman, et al.; Endocrinology 146, 2462 (2005) Abstract; Full Text
Related Products
Further Categories Containing This Product:
MPTP [Mitochondrial Transition Pore] / Related ProductsPolyclonal Antibodies
 
 
ALX-270-293 Revised 28-Mar-08
Decylubiquinone
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SYNONYMS 2,3-Dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Coenzymes & Vitamins Other Products
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ALX-270-293-M010   10 mg 60.00 USD Add To Cart
Product Specification
FORMULA: C19H30O4
MW: 322.4
CAS NUMBER: 55486-00-5
PURITY: ≥97% (TLC)
APPEARANCE: Clear orange to red liquid.
SOLUBILITY: Soluble in DMSO (10mg/ml) or TRIS buffer.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions in DMSO are stable for up to 3 months when stored at -20°C.
HAZARD: IRRITANT.

Product Description
Inhibitor of the mitochondrial permeability transition pore (MPTP). This inhibitory effect can be counteracted by ubiquinone-5 (Prod. No. ALX-270-294).
Product Specific Literature References
A ubiquinone-binding site regulates the mitochondrial permeability transition pore: E. Fontaine, et al.; J. Biol. Chem. 273, 25734 (1998) Abstract; Full Text
The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c: N.O. Karpinich, et al.; J. Biol. Chem. 277, 16547 (2002) Abstract; Full Text
Further Categories Containing This Product:
MPTP [Mitochondrial Transition Pore] / Related Products
 
 
ALX-270-294 Revised 03-May-06
Ubiquinone-5
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SYNONYMS Coenzyme Q1
2,3-Dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Coenzymes & Vitamins Other Products
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ALX-270-294-M002   2 mg 75.00 USD Add To Cart
Product Specification
FORMULA: C14H18O4
MW: 250.3
CAS NUMBER: 727-81-1
PURITY: ≥95% (HPLC)
APPEARANCE: Orange-red to red liquid.
SOLUBILITY: Soluble in acetone.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Counteracts inhibitory effects on mitochondrial permeability transition pore (MPTP) of decylubiquinone (Prod. No. ALX-270-293) and ubiquinone-10 (Prod. No. ALX-270-295).
Further Categories Containing This Product:
MPTP [Mitochondrial Transition Pore] / Related Products
 
 
ALX-270-295 Revised 03-May-06
Ubiquinone-10
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SYNONYMS Coenzyme Q2
2,3-Dimethoxy-5-methyl-geranyl-1,4-benzoquinone
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Coenzymes & Vitamins Other Products
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ALX-270-295-M002   2 mg 125.00 USD Add To Cart
Product Specification
FORMULA: C19H26O4
MW: 318.4
CAS NUMBER: 606-06-4
PURITY: ≥90% (HPLC)
APPEARANCE: Red liquid.
SOLUBILITY: Soluble in acetone.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C

Product Description
Inhibitor of the mitochondrial permeability transition pore (MPTP). This inhibitory effect can be counteracted by ubiquinone-5 (Prod. No. ALX-270-294).
Further Categories Containing This Product:
MPTP [Mitochondrial Transition Pore] / Related Products
 
 
ALX-302-010 Revised 28-Aug-07
Ganglioside GD3 . disodium salt (bovine brain)
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SYNONYMS Disialoganglioside GD3 . 2Na (bovine brain)
GD3 . 2Na (bovine brain)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Gangliosides / Related Products
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ALX-302-010-MC05   0.5 mg 150.00 USD Add To Cart
ALX-302-010-M001   1 mg 260.00 USD Add To Cart
Product Specification
SEQUENCE: [Il3(Neu5Ac)2LacCer] [[α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)]-β-Gal-(1→4)-β-Glc-(1→1')-Cer]
FORMULA: C70H123N3O29 . 2Na
MW: 1470.8 . 46.0 (calculated on sphingosine C18:1 and stearic acid)
CAS NUMBER: 62010-37-1
PURITY: ≥98% (TLC)
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Induces mitochondrial permeability transition (MPT) without requiring elevated Ca2+ levels and thus triggers Fas-mediated apoptosis.
Product Specific Literature References
Apoptogenic ganglioside GD3 directly induces the mitochondrial permeability transition: B.S. Kristal & A.M. Brown; J. Biol. Chem. 274, 23169 (1999) Abstract; Full Text
Lipid signaling in CD95-mediated apoptosis: F. Malisan & R. Testi; FEBS Lett 452, 100 (1999), (Review) Abstract
Ganglioside GD3, the mitochondrial permeability transition, and apoptosis: B.S. Kristal & A.M. Brown; Ann. NY Acad. Sci. 893, 321 (1999), (Review) Abstract
Ganglioside GD3 and its mimetics induce cytochrome c release from mitochondria: Y. Inoki, et al.; BBRC 276, 1210 (2000) Abstract
 
 
ALX-350-098 Revised 05-Apr-08
Ferutinin (high purity)
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SYNONYMS Tefestrol (high purity)
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Other Natural Products - DNA Regulation / Transcription
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ALX-350-098-M001   1 mg 35.00 USD Add To Cart
ALX-350-098-M005   5 mg 100.00 USD Add To Cart
ALX-350-098-M010   10 mg 170.00 USD Add To Cart
Product Specification
FORMULA: C22H30O4
MW: 358.5
SOURCE/HOST: Semisynthetic.
PURITY: ≥98%
SOLUBILITY: Soluble in acetone, dichloromethane, DMSO or ethyl acetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C

Product Description
Potent, naturally occuring non-steroid estrogenic compound. Agonist for estrogen receptor (ER) α and agonist/antagonist for ERβ with higher binding affinity than tamoxifen (Prod. No. ALX-550-095) for both ERs. Electrogenic Ca2+ ionophore inducing mitochondrial depolarisation which can be completely blocked by cyclosporin A (Prod. No. ALX-380-002), suggesting that ferutinin opens the mitochondrial permeability transition pore (mPTP). In a concentration range of 1-50µM ferutinin increases the permeability of thymocytes, mitochondria, sarcoplasmic reticulum, liposomes and bilayer lipid membranes for Ca2+.
Product Specific Literature References
Ferutinine structure: A.I. Saidkhodjaev, et al.; Chem. Nat. Comp. 1, 28 (1973)
Ionophoretic properties of ferutinin: M.V. Zamaraeva, et al.; Cell Calcium 22, 235 (1997) Abstract
Influence of plant terpenoids on the permeability of mitochondria and lipid bilayers: A.Y. Abramov, et al.; Biochim. Biophys. Acta 1512, 98 (2001) Abstract
Daucane phytoestrogens: a structure-activity study: G. Appendino, et al.; J. Nat. Prod. 65, 1612 (2002) Abstract
Terpenoids found in the umbelliferae family act as agonists/antagonists for ER(alpha) and ERbeta: differential transcription activity between ferutinine-liganded ER(alpha) and ERbeta: K. Ikeda, et al.; BBRC 291, 354 (2002) Abstract
Actions of ionomycin, 4-BrA23187 and a novel electrogenic Ca2+ ionophore on mitochondria in intact cells: A.Y. Abramov & M.R. Duchen; Cell Calcium 33, 101 (2003) Abstract
 
 
ALX-350-277 Revised 03-Apr-08
Betulinic acid (high purity)
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SYNONYMS 3β-Hydroxy-20(29)-lupaene-28-oic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antitumor Reagents
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ALX-350-277-M005   5 mg 32.00 USD Add To Cart
ALX-350-277-M025   25 mg 95.00 USD Add To Cart
ALX-350-277-M100   100 mg 290.00 USD Add To Cart
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Product Specification
FORMULA: C30H48O3
MW: 456.7
CAS NUMBER: 472-15-1
SOURCE/HOST: Isolated from Platanus acerifolia (plane) tree bark.
PURITY: ≥99%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep cool and dry.
HAZARD: IRRITANT.

Product Description
Antitumor and anti-HIV agent. Induces apoptosis by activating mitochondrial permeability transition (MPT). Inhibits NF-κB activation and NF-κB-regulated gene expression induced by carcinogens and inflammatory stimuli. Decreases expression of Bcl-2 and cyclin D1. Potent proteasome activator.
Product Specific Literature References
Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids: T. Fujioka, et al.; J. Nat. Prod. 57, 243 (1994) Abstract
Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis: E. Pisha, et al.; Nat. Med. 1, 1046 (1995) Abstract
Betulinic acid and dihydrobetulinic acid derivatives as potent anti-HIV agents: Y. Kashiwada, et al.; J. Med. Chem. 39, 1016 (1996) Abstract
Betulinic acid triggers CD95 (APO-1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors: S. Fulda, et al.; Cancer Res. 57, 4956 (1997) Abstract
Anti-AIDS agents--XXVII. Synthesis and anti-HIV activity of betulinic acid and dihydrobetulinic acid derivatives: F. Hashimoto, et al.; Bioorg. Med. Chem. 5, 2133 (1997) Abstract
Induction of p53 without increase in p21WAF1 in betulinic acid-mediated cell death is preferential for human metastatic melanoma: M. Rieber & M. Strasberg Rieber; DNA Cell Biol. 17, 399 (1998) Abstract
Betulinic acid induces apoptosis in human neuroblastoma cell lines: M.L. Schmidt, et al.; Eur. J. Cancer 33, 2007 (1997) Abstract
Activation of mitochondria and release of mitochondrial apoptogenic factors by betulinic acid: S. Fulda, et al.; J. Biol. Chem. 273, 33942 (1998) Abstract; Full Text
Betulinic acid inhibits aminopeptidase N activity: M.F. Melzig & H. Bormann; Planta Med. 64, 655 (1998) Abstract
Correspondence re: S. Fulda et al., Betulinic acid triggers CD95 (Apo1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors. Cancer Res., 57: 4956, 1997: M. Rieber & M. Strasberg Rieber; Cancer. Res. 58, 5876 (1998) Abstract
Betulinic acid: a new cytotoxic agent against malignant brain-tumor cells: S. Fulda, et al.; Int. J. Cancer 82, 435 (1999) Abstract
Betulinic acid-induced apoptosis in glioma cells: A sequential requirement for new protein synthesis, formation of reactive oxygen species, and caspase processing: W. Wick, et al.; J Pharmacol. Exp. Ther. 289, 1306 (1999) Abstract; Full Text
Effects of betulinic acid alone and in combination with irradiation in human melanoma cells: E. Selzer, et al.; J. Invest. Dermatol. 114, 935 (2000) Abstract
Betulinic acid suppresses carcinogen-induced NF-kappa B activation through inhibition of I kappa B alpha kinase and p65 phosphorylation: abrogation of cyclooxygenase-2 and matrix metalloprotease-9: Y. Takada & B.B. Aggarwal; J. Immunol. 171, 3278 (2003) Abstract
Inhibition of IkappaB kinase activity by acetyl-boswellic acids promotes apoptosis in androgen-independent PC-3 prostate cancer cells in vitro and in vivo: T. Syrovets, et al.; J. Biol. Chem. 280, 6170 (2005) Abstract
Induction of central signalling pathways and select functional effects in human platelets by beta-boswellic acid: D. Poeckel, et al.; Br. J. Pharmacol. 146, 514 (2005) Abstract
Betulinic acid and its derivatives: a review on their biological properties: P. Yogeeswari and D. Sriram; Curr. Med. Chem. 12, 657 (2005), Review Abstract
Betulinic acid and its derivatives, potent DNA topoisomerase II inhibitors, from the bark of Bischofia javanica: S. Wada & R. Tanaka; Chem. Biodivers. 2, 689 (2005) Abstract
Betulinic acid as new activator of NF-kappaB: molecular mechanisms and implications for cancer therapy: H. Kasperczyk, et al.; Oncogene 24, 6945 (2005) Abstract
Activation and inhibition of the proteasome by betulinic acid and its derivatives: L. Huang, et al.; FEBS Lett. 581, 4955 (2007) Abstract
 
 
ALX-350-298 Revised 03-Apr-08
Betulinic acid (~95%)
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SYNONYMS 3β-Hydroxy-20(29)-lupaene-28-oic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antitumor Reagents
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ALX-350-298-M100   100 mg 55.00 USD Add To Cart
ALX-350-298-M500   500 mg 180.00 USD Add To Cart
ALX-350-298-G001   1 g 290.00 USD Add To Cart
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Product Specification
FORMULA: C30H48O3
MW: 456.7
CAS NUMBER: 472-15-1
SOURCE/HOST: Isolated from Platanus acerifolia (plane tree) bark.
PURITY: ≥95% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C

Product Description
Antitumor and anti-HIV agent. Induces apoptosis by activating mitochondrial permeability transition (MPT). Inhibits NF-κB activation and NF-κB-regulated gene expression induced by carcinogens and inflammatory stimuli. Decreases expression of Bcl-2 and cyclin D1. Potent proteasome activator.
Product Specific Literature References
Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids: T. Fujioka, et al.; J. Nat. Prod. 57, 243 (1994) Abstract
Discov