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ALX-804-017
Revised 03-Mar-05
Monoclonal Antibody to Calmodulin (6D4)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calmodulin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-017-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Rat
Others
CLONE:
6D4
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified calmodulin from
Dictyostelium discoideum
strain.
SPECIFICITY:
Recognizes rat, bovine, chicken, chlamydomonas and dictyostelium calmodulin. Detects a band of ~17kDa by Western blot. Does not cross-react with parvalbumin, troponin, S-100 and myosin light chain kinase (MLCK).
APPLICATION:
ELISA
Immunocytochemistry (1:100)
Western Blot (1:2'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum:
D. Hulen, et al.; Cell Motility & Cytoskeleton
18
, 113 (1991)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in dictyostelium cells with Prod. No.
ALX-804-017
results in staining of the contractile vacuoles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-014
Revised 20-Oct-08
Monoclonal Antibody to Calnexin (AF18)
SYNONYMS
anti-p88 MAb (AF18)
anti-IP90 MAb (AF18)
PRODUCT LINE
Stress & Heat Shock Proteins
PRODUCT CATEGORY
Endoplasmatic Reticulum Stress
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-014-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
AF18
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Focus human hepatoma cell line.
SPECIFICITY:
Recognizes human and mouse calnexin. Detects a band of ~90kDa by Western blot.
APPLICATION:
Immunocytochemistry (1:500)
Immunoprecipitation
Western Blot (1:4’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene:
K. Galvin, et al.; PNAS
89
, 8452 (1992)
Abstract
;
Full Text
Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm:
P.P. Li, et al.; PNAS
99
, 1353 (2002)
Abstract
;
Full Text
FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction:
B.N. Desai, et al.; PNAS
99
, 4319 (2002)
Abstract
;
Full Text
c-erbB-3: a nuclear protein in mammary epithelial cells:
M. Offterdinger, et al.; J. Cell Biol.
157
, 929 (2002)
Abstract
;
Full Text
Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator:
H. Zhang, et al.; J. Biol. Chem.
277
, 28948 (2002)
Abstract
;
Full Text
Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex:
J. Enninga, et al.; Mol. Cell. Biol.
23
, 7271 (2003)
Abstract
;
Full Text
Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER:
R. Khanna, et al.; J. Cell Sci.
117
, 2897 (2004)
Abstract
;
Full Text
An LQT mutant minK alters KvLQT1 trafficking:
A. Krumerman, et al.; Am. J. Physiol. Cell Physiol.
286
, C1453 (2004)
Abstract
The stop transfer sequence of the human UDP-glucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms:
L. Barre, et al.; Febs J.
272
, 1063 (2005)
Abstract
;
Full Text
The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma:
K.T. Chin, et al.; Nucl. Acids Res.
33
, 1859 (2005)
Abstract
;
Full Text
Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis:
H. Zhang, et al.; J. Biol. Chem.
281
, 11312 (2006)
Abstract
;
Full Text
General Information
Calnexin, also referred to as IP90 or p88, is a ~88kDa chaperone protein found in the endoplastmic reticulum. It binds with partially folded immunoglobulin proteins and retains them in the endoplastmic reticulum until they are completely folded.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-053
Revised 11-Jan-05
Monoclonal Antibody to Calpain (156)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-053-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE:
156
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified 28kDa calpain subunit from bovine skeletal muscle.
SPECIFICITY:
Recognizes human and bovine 28kDa calpain subunit. Detects a band of ~28kDa by Western blot. Does not cross-react with the human 80kDa m-calpain subunit.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (µ-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
µ- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
Product Specific Literature References
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains:
J. Cong, et al.; J. Biol. Chem.
268
, 25740 (1993)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-051
Revised 09-Oct-08
Monoclonal Antibody to µ-Calpain (Domain II) (2H2A7C2)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-051-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Pig
Others
CLONE:
2H2A7C2
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine µ-calpain.
SPECIFICITY:
Recognizes an epitope in domain II (aa 245-265) of µ-calpain from several mammalian sources (e.g. human, rat, pig, bovine). Detects aband of ~80kDa by Western blot. Does not cross-react withm-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:
Immunocytochemistry (1:25)
Western Blot (1:1'000)
Note:
Not recommended for immunoprecipitation.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
The calpain (Ca
2+
-dependent proteinase or Ca
2+
-activated neutral protease) system consists of two ubiquitous forms of calpain (m-calpain and µ-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered Ca
2+
homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
m- and µ-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of Ca
2+
, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
BACKGROUND/TECHNICAL INFORMATION
Immunocytochemical staining of µ-calpain in pig LLC-PK1 cells with Prod. No. ALX-804-051 results in diffuse cytoplasmic staining.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-050
Revised 10-Nov-08
Monoclonal Antibody to µ-Calpain (Domain III) (9A4H8D3)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-050-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Others
CLONE:
9A4H8D3
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine µ-calpain.
SPECIFICITY:
Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine, hamster, rabbit). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:
Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections)
Western Blot (1:2’000)
Note:
Not recommended for immunoprecipitation.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen. Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with 804-050 results in diffuse cytoplasmic staining.
Product Specific Literature References
A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells:
R.D. Lane, et al.; Exp. Cell Res.
203
, 5 (1992)
Abstract
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains:
J. Cong, et al.; J. Biol. Chem.
268
, 25740 (1993)
Abstract
;
Full Text
Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25:
G. Kusakawa, et al.; J. Biol. Chem.
275
, 17166 (2000)
Abstract
;
Full Text
Calpain is a major cell death effector in selective striatal degeneration induced in vivo by 3-nitropropionate: implications for Huntington’s disease:
N. Bizat, et al.; J. Neurosci.
23
, 5020 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-055
Revised 11-Aug-08
Monoclonal Antibody to Calpastatin (Domain II) (2G11D6)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-055-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
Pig
Others
CLONE:
2G11D6
ISOTYPE:
Mouse IgG2a
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine skeletal muscle calpastatin.
SPECIFICITY:
Recognizes an epitope in domain II (aa 295-501) of human, rat, dog, pig, bovine and rabbit calpastatin. Detects bands of ~150, ~125, ~90 and ~70kDa by Western blot, which have been suggested to correspond to protein isoforms translated from different start sites. Does not cross-react with calpains or calmodulin.
APPLICATION:
Immunocytochemistry (1:1’000)
Immunohistochemistry (frozen sections)
Western Blot (1:5’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-054
Revised 04-Mar-08
Monoclonal Antibody to Calpastatin (Domain IV) (1F7E3D10)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-054-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Pig
Others
CLONE:
1F7E3D10
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine skeletal muscle 80kDa subunit of m-calpastatin.
SPECIFICITY:
Recognizes an epitope in domain IV (aa 543-673) of human, rat, pig and bovine calpastatin. Detects bands of ~150, ~125, ~90 and ~70kDa by Western blot, which have been suggested to correspond to protein isoforms translated from different start sites. Does not cross-react with calpains or calmodulin.
APPLICATION:
Immunocytochemistry (1:200)
Western Blot (1:5'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-089
Revised 27-Mar-08
Monoclonal Antibody to Calsequestrin (VIIID12)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Signalling (Intracellular) Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-089-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Dog
Others
CLONE:
VIIID12
ISOTYPE:
Mouse IgG2b
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified rabbit skeletal muscle sarcoplasmic reticulum.
SPECIFICITY:
Recognizes mouse, rat, rabbit and dog type I (slow) and type II (fast) calsequestrin. Detects a band of ~63kDa by Western blot.
APPLICATION:
Immunocytochemistry
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-399
Revised 01-Dec-05
Monoclonal Antibody to cAMP-dependent Protein Kinase Substrate (phosphorylated) (15B12)
SYNONYMS
anti-PKA Substrate (phosphorylated) MAb (15B12)
anti-Protein Kinase A Substrate (phosphorylated) MAb (15B12)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PKA Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-399-C100
100 µg
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE:
15B12
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:
Synthetic phosphopeptide corresponding to PKA (protein kinase A) substrate consensus phoshorylation site of activated PKA substrate.
SPECIFICITY:
Recognizes RRXpS motif of activated PKA substrate in a broad range of species.
APPLICATION:
ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note:
Not suitable for Western blot.
Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-388
Revised 20-Sep-07
Monoclonal Antibody to Casein Kinase II Substrate (phosphorylated) (9F4)
SYNONYMS
anti-CKII Substrate (phosphorylated) MAb (9F4)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Casein Kinase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-388-C100
100 µg
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE:
9F4
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic phosphopeptide corresponding to CKII (casein kinase II) substrate consensus phosphorylation site of activated CKII substrate.
SPECIFICITY:
Recognizes DpSD motif of activated CKII substrate in a broad range of species.
APPLICATION:
ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note:
Not suitable for Western blot!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-530
Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-530-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
1H11
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
