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ALX-201-402
Revised 27-Mar-08
SIGIRR (human):Fc (human) (recombinant)
SYNONYMS
Single Ig IL-1R-related Molecule (human):Fc (human) (recombinant)
TIR8 (human):Fc (human) (recombinant)
Toll/Interleukin-1 Receptor 8 (human):Fc (human) (recombinant)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Interleukin-1 [IL-1] & Receptors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-402-C010
10 µg
260.00 USD
ALX-201-402-C050
50 µg
560.00 USD
Product Specification
SOURCE/HOST:
Produced in HEK 293 cells. Signal peptide and extracellular domain of human SIGIRR (aa 1-118) are fused at the C-terminus to the Fc portion of human IgG.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:
<0.1EU/µg protein (LAL-test).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q6IA17:
SIGIRR (human)
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Proteasome / Related Products
ALX-202-047
Revised 16-Jun-08
Proteasome 20S (rabbit)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-202-047-C025
25 µg
88.00 USD
Product Specification
MW:
~700kDa.
SOURCE/HOST:
Isolated from rabbit.
CONCENTRATION:
1.4mg/ml (2μM)
PURITY:
≥95% (SDS PAGE)
FORMULATION:
Liquid. In 50mM HEPES, pH 7.6, containing 150mM sodium chloride and 1mM DTT.
APPLICATION:
Protein degradation assays. Typical enzyme concentration for assay is 0.1-1nM. Requires activation by SDS (0.035%) or PA28 Activator.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
20S proteasome is the catalytic core of the 26S proteasome that degrades polyubiquitinated proteins. Known to hydrolyze various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Detergent and PA28 activated. Activity is measured using the fluorogenic substrate Z-LLVY-AMC (Prod. No.
ALX-260-070
) with SDS activation.
Product Specific Literature References
Purification of two high molecular weight proteases from rabbit reticulocyte lysate:
R. Hough, et al.; J. Biol. Chem.
262
, 8303 (1987)
Abstract
;
Full Text
Degradation of oxidized insulin B chain by the multiproteinase complex macropain (proteasome):
L.R. Dick, et al.; Biochemistry
30
, 2725 (1991)
Abstract
The ubiquitin-proteasome proteolytic pathway:
A. Ciechanover; Cell
79
, 13 (1994)
Abstract
Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides:
L.R. Dick, et al.; J. Immunol.
152
, 3884 (1994)
Abstract
Kinetic characterization of the chymotryptic activity of the 20S proteasome:
R.L. Stein, et al.; Biochemistry
35
, 3899 (1996)
Abstract
Further Categories Containing This Product:
Enzymes
•
Natural Proteins
ALX-210-294
Revised 13-Mar-06
Polyclonal Antibody to Proteasome 19S (Subunit S2) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-294-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Purified total IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human proteasome 19S subunit S2.
SPECIFICITY:
Recognizes subunit S2, located in the 19S regulatory subunit of human 26S proteasome. Detects bands of ~100kDa (S2 subunit) and ~50kDa (subunit degradation product) by Western blot.
APPLICATION:
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-295
Revised 05-Nov-07
Polyclonal Antibody to Proteasome 20S (Subunit C2)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-295-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 249-263 (CP
249
ADEPAEKAD
EPMEH
263
) of C-terminal human proteasome 20S subunit C2.
SPECIFICITY:
Recognizes human, mouse, rat, hamster and dog 20S subunit C2. Detects a band of ~29kDa by Western blot.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-049
.
General Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator, see Prod. No.
ALX-804-214
), Angelman’s syndrome (E6-AP, see Prod. No.
ALX-210-260
), and Liddle syndrome (endothelial sodium channels, see Prod. No.
ALX-210-291
,
ALX-210-300
or
ALX-210-299
).
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-296
Revised 21-Jan-05
Polyclonal Antibody to Proteasome 19S (Subunit S10B)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-296-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Purified IgG in PBS containing 1mg/ml BSA.
IMMUNOGEN:
Synthetic peptide corresponding to aa 278-294 (CA
278
TNRPD
TLDPALLRPGR
294
) of human proteasome 19S subunit 10B.
SPECIFICITY:
Recognizes subunit S10B located in the 19S regulatory subunit of human, mouse, rat, dog and hamster 26S Proteasome. Detects bands of ~42, ~46, ~48 and ~52kDa by Western blot, which correspond to subunit 10B and probably the combined signal from 4, 6B, 7 and 8 subunit proteins.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-050
.
Product Description
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator, see Prod. No.
ALX-804-214
), Angelman's syndrome (E6-AP, see Prod. No.
ALX-210-260
), and Liddle syndrome (endothelial sodium channels, see Prod. No.
ALX-210-291
,
ALX-210-300
or
ALX-210-299
).
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-297
Revised 21-Jan-05
Polyclonal Antibody to Proteasome Activator 11S (Subunit REGγ) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-297-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Epitope-affinity purified IgG containing 1mg/ml BSA.
IMMUNOGEN:
Synthetic peptide corresponding to aa 1-14 (M
1
ASLLKVDQE
VKLK
14
C) of human proteasome activator 11S subunit REGγ.
SPECIFICITY:
Recognizes human 11S subunit REGγ. Detects a band of 28kDa by Western blot.
APPLICATION:
Western Blot (1:1'000).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-047
.
Product Description
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator, see Prod. No.
ALX-804-214
), Angelman's syndrome (E6-AP, see Prod. No.
ALX-210-260
), and Liddle syndrome (endothelial sodium channels, see Prod. No.
ALX-210-291
,
ALX-210-300
or
ALX-210-299
).
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-298
Revised 04-Jun-07
Polyclonal Antibody to Proteasome Activator 11S (Subunit REGα)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-298-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 5-22 (R
5
VQPEAQAKVDV
FREDLC
22
) of human proteasome activator 11S subunit REGα.
SPECIFICITY:
Recognizes human, mouse, rat, dog and hamster proteasome activator 11S subunit REGα. Detects a band of ~28kDa by Western blot.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-048
.
Product Description
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in, cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator, see Prod. No.
ALX-804-214
), Angelman's syndrome (E6-AP, see Prod. No.
ALX-210-260
), and Liddle syndrome (endothelial sodium channels, see Prod. No.
ALX-210-291
,
ALX-210-300
or
ALX-210-299
).
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-319
Revised 12-Apr-07
Polyclonal Antibody to Proteasome 19S (Subunit S4) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-319-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Purified total IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant proteasome 19S subunit S4.
SPECIFICITY:
Recognizes subunit S4 located in the 19S regulatory subunit of human 26S proteasome. Detects a band of ~56kDa by Western blot. Also detects a weak band of ~35kDa, which may correspond to subunit degradation product.
APPLICATION:
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-320
Revised 10-Oct-05
Polyclonal Antibody to Proteasome 19S (Subunit S5A) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-320-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Purified total IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human proteasome 19S subunit S5A.
SPECIFICITY:
Recognizes subunit S5A located in the 19S regulatory subunit of human 26S proteasome. Detects a band of ~50kDa by Western blot.
APPLICATION:
Immunoprecipitation
Western Blot (1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-321
Revised 12-Jan-05
Polyclonal Antibody to Proteasome 19S (Subunit S6) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-321-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Purified total IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human proteasome 19S subunit S6.
SPECIFICITY:
Recognizes subunit S6 located in the 19S regulatory subunit of human 26S proteasome. Detects a band of ~48kDa by Western blot.
APPLICATION:
Immunoprecipitation
Western Blot (1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-322
Revised 12-Jan-05
Polyclonal Antibody to Proteasome 19S (Subunit S6') (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-322-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Purified total IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human proteasome 19S subunit S6'.
SPECIFICITY:
Recognizes subunit S6', located in the 19S regulatory subunit of human 26S proteasome. Detects a band of ~48kDa by Western blot.
APPLICATION:
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-323
Revised 08-Feb-05
Polyclonal Antibody to Proteasome 19S (Subunit S7) (human)
PRODUCT LINE
Ubiquitin & Proteasome
PRODUCT CATEGORY
Proteasome / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-323-R100
100 µl
379.00 USD
