ALEXIS Biochemicals: HSP104 (yeast) (recombinant)

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ALX-201-135

Revised 15-Oct-08

Caspase-2 (active) (human) (recombinant) (His)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-135-C005		5 µg	255.00 USD

Product Specification

MW:	Bands at ~10 and at ~20kDa can be detected by Western blot.
EC:	3.4.22.55
SOURCE/HOST:	Produced in E. coli. Human caspase 2 (aa 18-450) is fused at the C-terminus to a His-tag. When expressed in E. coli, caspase-2 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
CONCENTRATION:	0.2mg/ml
FORMULATION:	Liquid. In 50mM TRIS, pH 8.0, containing 100mM sodium chloride and 50mM imidazole.
ACTIVITY:	The rate of caspase-2 enzymatic hydrolysis can be measured by the release of AFC from the caspase substrate Ac-VDVAD-AFC (Prod. No. ALX-260-112) as emission at 505nm and excitation at 400nm using a spectrofluorometer.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least one week when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

General Information

When expressed in E. coli, caspase-2 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-2 enzymatic activity can be measured by the release of AFC from the caspase substrate Ac-VDVAD-AFC (Prod. No. ALX-260-112) as emission at 505nm and excitation at 400nm using a spectrofluorometer.

BACKGROUND/TECHNICAL INFORMATION

Enzyme Evaluation Protocol
This protocol is used to measure caspase enzyme activity. The synthetic fluorogenic peptide Ac-VDVAD-AFC (Prod. No. ALX-260-112) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AFC (7-amino-4-trifluoromethylcoumarin). AFC release can be measured by spectrofluorometry using UV.

1. Add 20mM of Ac-VDVAD-AFC (Prod. No. ALX-260-112) to 1ml of assay buffer (20mM PIPES, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose, pH 7.2). Add the appropriate amount of active caspase to the mixture (50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AFC liberated from the Ac-VDVAD-AFC using a spectrofluorometer with an excitation wavelength of 400nm and an emission wavelength of 505nm.

Note: Enzyme activity can also be measured using the caspase enzyme substrate Ac-VDVAD-pNA (Prod. No. ALX-260-059) at 37°C in a spectromax at 405nm wavelength.

Swiss-Prot link P42575: Caspase-2 (human) (precursor)
AfCS Signalling Gateway link A000497: Caspase-2 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-136

Revised 14-Aug-08

Caspase-9 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-136-C005		5 µg	255.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.2mg/ml
FORMULATION:	Liquid. In 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least one week when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997) Abstract; Full Text

General Information

When expressed in E. coli, caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme. The rate of caspase-9 enzymatic activity can be measured by the release of AMC from the caspase substrate Ac-LEHD-AMC (Prod. No. ALX-260-080) as emission at 440nm and excitation at 380nm using a spectrofluorometer.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P55211: Caspase-9 (human) (precursor) AfCS Signalling Gateway link A000504: Caspase-9 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-137

Revised 16-Feb-05

Caspase-10 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-137-C005		5 µg	255.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
FORMULATION:	Liquid. 5µg in 25µl of 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least one week when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999) Abstract

General Information

When expressed in E. coli, caspase-10 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-10 enzymatic activity can be measured by the release of AMC from the caspase substrate Ac-IETD-AMC (Prod. No. ALX-260-042) as emission at 440nm and excitation at 380nm using a spectrofluorometer.

BACKGROUND/TECHNICAL INFORMATION

Enzyme Evaluation Protocol
This protocol is used to measure caspase enzyme activity. The synthetic fluorogenic peptide Ac-IETD-AMC (Prod. No. ALX-260-042) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC (7-amino-4-methylcoumarin). AMC release can be measured by spectrofluorometry using UV.

1. Add 20mM of Ac-IETD-AMC (Prod. No. ALX-260-042) to 1ml of assay buffer (20mM PIPES, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose, pH 7.2). Add the appropriate amount of active caspase to the mixture (50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AMC liberated from the Ac-IETD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm.

Note: Enzyme activity can also be measured using the caspase enzyme substrate Ac-IETD-pNA (Prod. No. ALX-260-045) at 37°C in a spectromax at 405nm wavelength.

Swiss-Prot link Q92851: Caspase-10 (human) (precursor)
AfCS Signalling Gateway link A000493: Caspase-10 (human)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

ALX-201-138

Revised 11-Sep-08

HSP90 (yeast) (recombinant) (His)
SYNONYMS	Heat Shock Protein 90 (yeast) (recombinant) HSP82 (yeast) (recombinant) Heat Shock Protein 82 (yeast) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	HSP90 / HtpG / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-138-C025		25 µg	180.00 USD
ALX-201-138-C100		100 µg	630.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli. Fused at the N-terminus to a His-tag.
CONCENTRATION:
PURITY:	≥99%
FORMULATION:	Liquid. In 40mM HEPES/KOH, pH 7.5, containing 150mM KCl.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Specific Literature References

Purification and characterization of prokaryotic and eukaryotic Hsp90: J. Buchner, et al.; Methods Enzymol. 290, 409 (1998) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P02829: Heat shock protein HSP82 (Saccharomyces cerevisiae (Baker's yeast))

General Literature References

Hsp90: chaperoning signal transduction: K. Richter & J. Buchner; J. Cell. Physiol. 188, 281 (2001), (Review) Abstract; Full Text

Further Categories Containing This Product:

Chaperone-mediated Autophagy [CMA] • Recombinant Proteins / Fusion Proteins

ALX-201-139

Revised 18-May-05

HSP26 (yeast) (recombinant)
SYNONYMS	Heat Shock Protein 26 (yeast) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	Small Heat Shock Proteins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-139-C025		25 µg	155.00 USD

Product Specification

SOURCE/HOST:	Produced in yeast.
CONCENTRATION:
PURITY:	≥99%
FORMULATION:	Liquid. In 40mM HEPES, pH 7.4, containing 50mM NaCl, 2mM EDTA and 1mM DTE.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Product Specific Literature References

Hsp26: a temperature-regulated chaperone: M. Haslbeck, et al.; EMBO J. 18, 6744 (1999) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P15992: Heat shock protein 26 (Saccharomyces cerevisiae (Baker's yeast))

General Literature References

Chaperone function of sHsps: M. Haslbeck & J. Buchner; Prog. Mol. Subcell. Biol. 28, 37 (2002), (Review) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-140

Revised 02-May-05

HSP12 (yeast) (recombinant)
SYNONYMS	Heat Shock Protein 12 (yeast) (recombinant) Glucose and Lipid-regulated Protein (yeast) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	Small Heat Shock Proteins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-140-C025		25 µg	155.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.9mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 40mM HEPES, pH 7.4, containing 50mM NaCl, 1mM EDTA and 1mM DTE.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Product Specific Literature References

HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function: U.M. Prakelt & P.A. Meacock; Mol. Gen. Genet. 223, 97 (1990) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P22943: 12 kDa heat shock protein (Saccharomyces cerevisiae (Baker's yeast))

General Literature References

Chaperone function of sHsps: M. Haslbeck & J. Buchner; Prog. Mol. Subcell. Biol. 28, 37 (2002), (Review) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-141

Revised 02-Apr-08

GroEL (E. coli) (recombinant)
SYNONYMS	Heat Shock Protein 60 (E. coli) (recombinant) HSP60 (E. coli) (recombinant) 60kDa Chaperonin (E. coli) (recombinant) Protein Cpn60 GroEL Protein (E. coli) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	HSP60 / GroEL / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-141-C025		25 µg	155.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	26mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 50mM TRIS/HCl, pH 8.0, containing 10mM KCl and 10mM MgCl₂.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Full activity after short incubation at room temperature.
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Product Specific Literature References

Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes: O. Fayet, et al.; Mol. Gen. Genet. 202, 435 (1986) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P06139: 60kDa chaperonin (E. coli)

General Literature References

Structure and function of the GroE chaperone: S. Walter, et al.; Cell. Mol. Life Sci. 59, 1589 (2002), (Review) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-142

Revised 10-Feb-05

GroES (E. coli) (recombinant)
SYNONYMS	Heat Shock Protein 10 (E. coli) (recombinant) HSP10 (E. coli) (recombinant) 10kDa Chaperonin (E. coli) (recombinant) Protein Cpn10 GroES Protein (E. coli) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	HSP60 / GroEL / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-142-C025		25 µg	155.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	4.2mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 50mM TRIS/HCl, pH 8.0, containing 10mM KCl and 10mM MgCl₂.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Specific Literature References

Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes: O. Fayet, et al.; Mol. Gen. Genet. 202, 435 (1986) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P05380: 10kDa chaperonin (E. coli)

General Literature References

Structure and function of the GroE chaperone: S. Walter; Cell. Mol. Life Sci. 59, 1589 (2002), (Review) Abstract

Further Categories Containing This Product:

Co-Chaperones & HSP-interacting Proteins / Related Products • Recombinant Proteins / Fusion Proteins

ALX-201-143

Revised 18-May-05

DnaK (E. coli) (recombinant)
SYNONYMS	Heat Shock Protein 70 (E. coli) (recombinant) HSP70 (E. coli) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	HSP70 / DnaK / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-143-C025		25 µg	135.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:
PURITY:	≥99%
FORMULATION:	Liquid. In 20mM TRIS/HCl, pH 7.2, containing 100mM NaCl, 40mM KCl, 0.1mM EDTA, 1mM DTT and 5% glycerol.
APPLICATION:	Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Product Specific Literature References

The unfolding story of the Escherichia coli Hsp70 DnaK: is DnaK a holdase or an unfoldase?: S.V. Slepenkov & S.N. Witt; Mol. Microbiol. 45, 1197 (2002), (Review) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P04475: Chaperone protein dnaK (E. coli)

General Literature References

Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question: D.A. Dougan, et al.; Cell. Mol. Life Sci. 59, 1607 (2002), (Review) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-144

Revised 08-Jun-06

DnaJ (E. coli) (recombinant)
SYNONYMS	Heat Shock Protein 40 (E. coli) (recombinant) HSP40 (E. coli) (recombinant) Heat Shock Protein J (E. coli) (recombinant)
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	Co-Chaperones & HSP-interacting Proteins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-144-C025		25 µg	290.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	4.3mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 30mM HEPES/KOH, pH 8.2, containing 120mM KCl, 15mM NaCl and 4% glycerol.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Specific Literature References

Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK: K. Liberek, et al.; PNAS 88, 2874 (1991) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P08622: Chaperone protein dnaJ (E. coli)

General Literature References

Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question: D.A. Dougan, et al.; Cell. Mol. Life Sci. 59, 1607 (2002), (Review) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-145

Revised 08-Oct-08

GrpE (E. coli) (recombinant)
SYNONYMS	HSP24 (E. coli) (recombinant) HSP70 Cofactor (E. coli) (recombinant) Nucleotide Exchange Factor (E. coli) (recombinant)