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Natural Killer T [NKT] Cells / Related Products
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ALX-302-010 Revised 28-Aug-07
Ganglioside GD3 . disodium salt (bovine brain)
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SYNONYMS Disialoganglioside GD3 . 2Na (bovine brain)
GD3 . 2Na (bovine brain)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Gangliosides / Related Products
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ALX-302-010-MC05   0.5 mg 150.00 USD Add To Cart
ALX-302-010-M001   1 mg 260.00 USD Add To Cart
Product Specification
SEQUENCE: [Il3(Neu5Ac)2LacCer] [[α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)]-β-Gal-(1→4)-β-Glc-(1→1')-Cer]
FORMULA: C70H123N3O29 . 2Na
MW: 1470.8 . 46.0 (calculated on sphingosine C18:1 and stearic acid)
CAS NUMBER: 62010-37-1
PURITY: ≥98% (TLC)
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Induces mitochondrial permeability transition (MPT) without requiring elevated Ca2+ levels and thus triggers Fas-mediated apoptosis.
Product Specific Literature References
Apoptogenic ganglioside GD3 directly induces the mitochondrial permeability transition: B.S. Kristal & A.M. Brown; J. Biol. Chem. 274, 23169 (1999) Abstract; Full Text
Lipid signaling in CD95-mediated apoptosis: F. Malisan & R. Testi; FEBS Lett 452, 100 (1999), (Review) Abstract
Ganglioside GD3, the mitochondrial permeability transition, and apoptosis: B.S. Kristal & A.M. Brown; Ann. NY Acad. Sci. 893, 321 (1999), (Review) Abstract
Ganglioside GD3 and its mimetics induce cytochrome c release from mitochondria: Y. Inoki, et al.; BBRC 276, 1210 (2000) Abstract
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MPTP [Mitochondrial Transition Pore] / Related Products • Natural Killer T [NKT] Cells / Related Products
 
 
ALX-306-030 Revised 06-Dec-06
Globotrihexosylceramide
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SYNONYMS Gb3
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-030-C100   100 µg 85.00 USD Add To Cart
ALX-306-030-M001   1 mg 455.00 USD Add To Cart
Product Specification
FORMULA: C62H117NO18
MW: 1164.6
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in pyridine or chloroform:methanol (2:1).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C

Product Description
Negative control for isoglobotrihexosylceramide (iGb3) (Prod. No. ALX-306-028). Does not stimulate natural killer T (NKT) cells.
Product Specific Literature References
Chemoenzymatic Syntheses of iGb3 and Gb3: Q. Yao et al.; Org. Lett. 8, 911 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Solubilization:
Our recommendation is to dissolve product in chloroform: abs. methanol 2:1. Aliquot into glass tubes, dry and store at 4°C. Resuspended in abs. methanol (at concentrations not higher than 1%) prior to cell assays.
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Further Categories Containing This Product:
Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-306-028 Revised 05-May-08
Isoglobotrihexosylceramide
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SYNONYMS iGb3
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-306-028-C100   100 µg 85.00 USD Add To Cart
ALX-306-028-M001   1 mg 455.00 USD Add To Cart
Product Specification
FORMULA: C62H117NO18
MW: 1164.6
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in pyridine or chloroform:methanol (2:1).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C

Product Description
Lysosomal glycosphingolipid proposed to be the natural ligand of NKT cells. Stimulates natural killer T (NKT) cells similar to α-GalCer. For negative control compound see globotrihexosylceramide (Gb3) (Prod. No. ALX-306-030).
Product Specific Literature References
[1] Lysosomal glycosphingolipid recognition by NKT cells: D. Zhou, et al.; Science 306, 1786 (2004) Abstract
[2] Synthesis and biological evaluation of alpha-galactosylceramide (KRN7000) and isoglobotrihexosylceramide (iGb3): C. Xia, et al.; Bioorg. Med. Chem. Lett. 16, 2195 (2006) Abstract
[3] Chemoenzymatic Syntheses of iGb3 and Gb3: Q. Yao et al.; Org. Lett. 8, 911 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Solubilization:
Our recommendation is to dissolve product in chloroform: abs. methanol 2:1. Aliquot into glass tubes, dry and store at 4°C. Resuspended in abs. methanol (at concentrations not higher than 1%) prior to cell assays. Note: The use of DMSO may abolish the iGb3 activity and should be avoided [2].

For expanding human NKT cells from freshly isolated peripheral blood mononuclear cells (PBMC):
Mix iGb3 at 100ng/ml with PBMC and culture for 4-5 days.

For the stimulation of mouse NKT cells:
The bone marrow derived dendritic cells (BMDC) is the most efficient cell type that present iGb3. Use 50’000-250’000 of BMDCs pulsed overnight with 100ng/ml of iGb3 and 50’000 freshly purified NKT cells as a responder. Use tetramer sorting to purifiy the NKT cells from spleen and before mixing with BMDCs, rest them in culture medium for 12 hours and then wash with medium to avoid a high background. MACS sorting technique is not recommended. Alternatively, NKT cell hybridomas can be used to detect the ligand. (For reference see: http://home.uchicago.edu/~dzhou/Testing%20iGb3%20ligand.htm)
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Further Categories Containing This Product:
Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-306-031 Revised 23-Sep-08 New product
α-Mannosyl Ceramide
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SYNONYMS α-ManCer
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-031-C250   250 µg 120.00 USD Add To Cart
ALX-306-031-M001   1 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C50H99NO9
MW: 858.3
PURITY: ≥95%
APPEARANCE: White to brown powder.
SOLUBILITY: Soluble in pyridine or warm DMSO (4mg/ml).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, aliquot into glass vials.

Product Description
Potent stimulator of natural killer T (NKT) cells expressing the invariant Vα19-Jα26 T cell receptor α chain (Vα19 NKT cells).
Product Specific Literature References
Synthetic alpha-mannosyl ceramide as a potent stimulant for an NKT cell repertoire bearing the invariant Valpha19-Jalpha26 TCR alpha chain: N. Okamoto, et al.; Chem. Biol. 12, 677 (2005) Abstract
Induction of promotive rather than suppressive immune responses from a novel NKT cell repertoire Valpha19 NKT cell with alpha-mannosyl ceramide analogues consisting of the immunosuppressant ISP-I as the sphingosine unit: M. Shimamura, et al.; Eur. J. Med. Chem. 41, 569 (2006) Abstract
Modulation of Valpha19 NKT cell immune responses by alpha-mannosyl ceramide derivatives consisting of a series of modified sphingosines: M. Shimamura, et al.; Eur. J. Immunol. 37, 1836 (2007) Abstract
General Information
Natural killer T cells (NKT cells) are important regulators of the immune system. They share properties of both innate as well as adaptive immunity [1]. They express T cell receptors (TCR) on their surface consisting of an invariable α-chain and a somewhat variable β-chain [2]. The majority of NKT cells express the invariable α-chain Vα14-Jα18 (Vα14) in mouse or its homolog in humans Vα24-Jα18 (Vα24) [3]. Vα14 NKT cells recognize α-GalCer presented by CD1d [4, 5].
Recently, NKT cells expressing the invariant Vα19-Jα26 TCR α-chain (Vα19 NKT cells) have been found in peripheral blood lymphocytes and in the liver [6]. They recognize α-ManCer presented by MR1 (Major histocompatibility complex, class I-related) and respond with production of large amounts of immunoregulatory cytokines [7, 8].
General Literature References
[1] Mouse CD1-specific NK1 T cells: development, specificity, and function: A. Bendelac, et al.; Annu. Rev. Immunol. 15, 535 (1997) Abstract
[2] Germline-encoded recognition of diverse glycolipids by natural killer T cells: J.P. Scott-Browne, et al.; Nat. Immunol. 8, 1105 (2007) Abstract
[3] CD1d-restricted mouse V alpha 14 and human V alpha 24 T cells: lymphocytes of innate immunity: K. Benlagha & A. Bendelac; Semin. Immunol. 12, 537 (2000) Abstract
[4] CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides: T. Kawano, et al.; Science 278, 1626 (1997) Abstract
[5] CD1d-lipid-antigen recognition by the semi-invariant NKT T-cell receptor: N.A. Borg, et al.; Nature 448, 44 (2007) Abstract
[6] Presence of a novel subset of NKT cells bearing an invariant V(alpha)19.1-J(alpha)26 TCR alpha chain: M. Shimamura & Y.Y. Huang; FEBS Lett. 516, 97 (2002) Abstract
[7] Synthetic alpha-mannosyl ceramide as a potent stimulant for an NKT cell repertoire bearing the invariant Valpha19-Jalpha26 TCR alpha chain: N. Okamoto, et al.; Chem. Biol. 12, 677 (2005) Abstract
[8] Modulation of Valpha19 NKT cell immune responses by alpha-mannosyl ceramide derivatives consisting of a series of modified sphingosines: M. Shimamura, et al.; Eur. J. Immunol. 37, 1836 (2007) Abstract
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Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-804-564 Revised 10-Jun-08
Monoclonal Antibody to Fyn (FYN-01)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Src Family Kinases / Related Products
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ALX-804-564-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: FYN-01
ISOTYPE: Mouse IgG
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant of human Fyn (aa 7-176).
SPECIFICITY: Recognizes human and mouse Fyn. Detects a band of ~59kDa by immunoprecipitation.
APPLICATION: Immunocytochemistry
Immunoprecipitation: Preparation of cell lysate: 30 min. on ice (orbital incubator) in lysis buffer containing N-dodecyl-β-D-maltoside (Prod. No. ALX-500-007) (20mM TRIS-Cl, 100mM sodium chloride, pH 8.2, 1% laurylmaltoside, 50mM sodium fluoride); positive control J77 T-cell lysate.
Western Blot (1:500)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Monoclonal Antibodies • Natural Killer T [NKT] Cells / Related Products
 
 
ALX-804-246 Revised 10-Mar-08
Monoclonal Antibody to SLAM (human) (IPO-3)
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SYNONYMS anti-CD150 (human) MAb (IPO-3)
PRODUCT LINE Immunology
PRODUCT CATEGORY CD150 [SLAM; IPO-3] / Related Products
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ALX-804-246-C100   100 µg 395.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: IPO-3
ISOTYPE: Mouse IgG1
CONCENTRATION: ~1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.2. Contains no sodium azide.
IMMUNOGEN: Activated B cells.
SPECIFICITY: Recognizes human SLAM (CD150).
APPLICATION: ELISA (1:750)
Flow Cytometry (1:750)
Immunocytochemistry 
Immunohistochemistry (frozen sections (1:75))
Immunoprecipitation
Functional Application (Cross-linking of surface receptors)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies IPO-3 and IPO-10 against human B cell differentiation antigens: V.G. Pinchouk, et al.; Anticancer Res. 8, 1377 (1988) Abstract
Monoclonal antibodies of IPO series against B cell differentiation antigens in leukemia and lymphoma immunophenotyping: S.P. Sidorenko, et al.; Neoplasma 39, 3 (1992) Abstract
Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes: S.P. Sidorenko & E.A. Clark; J. Immunol. 151, 4614 (1993) Abstract
Immunodeficiency virus cDNA synthesis in resting T lymphocytes is regulated by T cell activation signals and dendritic cells: P.S. Polacino, et al.; J. Med. Primatol. 25, 201 (1996) Abstract
CDw150 associates with src-homology 2-containing inositol phosphatase and modulates CD95-mediated apoptosis: S.V. Mikhalap, et al.; J. Immunol. 162, 5719 (1999) Abstract; Full Text
SLAM (CDw150) is a cellular receptor for measles virus: H. Tatsuo, et al.; Nature 406, 893 (2000) Abstract
CD150 association with either the SH2-containing inositol phosphatase or the SH2-containing protein tyrosine phosphatase is regulated by the adaptor protein SH2D1A: L.M. Shlapatska, et al.; J. Immunol. 166, 5480 (2001) Abstract; Full Text
The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells: S.V. Mikhalap, et al.; Blood 104, 4063 (2004) Abstract; Full Text
General Information
SLAM (CDw150) is present on the surface of T and B cells, and is a self-ligand mediating T and B cell interactions. Absence of an inhibitor (SAP) which blocks recruitment of the signal transduction molecule SHP-2 to its docking site in the SLAM cytoplasmic region is associated with X-linked lymphoproliferative disease [1].
General Literature References
[1] The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM  : J. Sayos, et al.; Nature 395, 462 (1998) Abstract
Further Categories Containing This Product:
Natural Killer T [NKT] Cells / Related Products • Monoclonal Antibodies • CD Antibodies
 
 
ALX-306-029 Revised 21-Oct-08
OCH
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SYNONYMS (2S,3S,4R)-1-O-(α-D-Galactopyranosyl)-2-tetracosanoyl-
aminononan-3,4-diol
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-029-C250   250 µg 120.00 USD Add To Cart
ALX-306-029-M001   1 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C39H78NO9
MW: 704.1
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO, 100% ethanol (warm) or methanol (warm).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, aliquot into glass vials.

Product Description
Truncated analog of α-Gal-Cer. Selectively induces IL-4, as opposed to IFN-γ. Offers protection in mouse against experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Also offers protection against diabetes in NOD mice and against collagen induced arthritis. Stimulation of NKT cells with α-Gal-Cer results in release of a mixture of Th1 and Th2 cytokines, stimulation with OCH results in release of primarily Th2 cytokine.
Product Specific Literature References
Synthesis and evaluation of sphinganine analogues of KRN7000 and OCH: R.M. Ndonye, et al.; J. Org. Chem. 70, 10260 (2005) Abstract
Total synthesis of an immunosuppressive glycolipid, (2S,3S,4R)-1-O- (alpha-d-galactosyl)-2- tetracosanoylamino-1,3,4-nonanetriol: K. Murata, et al.; J. Org. Chem. 70, 2398 (2005) Abstract
Glycolipids for natural killer T cells: P.B. Savage, et al.; Chem. Soc. Rev. 351, 771 (2006) Abstract
Further Categories Containing This Product:
Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
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