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ALX-850-234 Revised 17-Apr-08
CD30, Soluble (human) ELISA Kit
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SYNONYMS Ki-1, Soluble (human) ELISA Kit
TNFSF 8, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-234-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.33ng/ml (range 1.6 to 100ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human sCD30 in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
HANDLING: Store controls at -20°C. Store rest of the kit at +4°C.
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits • CD Kits & Sets
 
 
ALX-850-066 Revised 23-Apr-08
CD30L, Soluble (human) (recombinant) Set
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SYNONYMS CD153, Soluble (human) (recombinant) Set
TNFSF 8, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-066-KI01   1 Set 460.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.
CONCENTRATION: CD30L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD30L, Soluble (human) (recombinant) (Prod. No. ALX-522-028) and 2x50µg Enhancer for Ligands (Prod. No. ALX-804-034).
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute CD30L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD30L binds to human CD30 (Ki-1).
APPLICATION: Can be used together with anti-FLAG® MAb to detect membrane-bound human CD30. Co-stimulates T cells in the presence of cross-linking enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Selective enrichment of CD30-expressing cells within the blast region of lymphocytes from patients with primary HIV infection (PHI): P. Biswas, et al.; J. Biol. Regul. Homeost. Agents 16, 33 (2002) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-850-262 Revised 11-Apr-08
CD40, Soluble (human) ELISA Kit
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SYNONYMS TNFSF 5, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-850-262-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 6.92pg/ml (range 7.8 to 500 pg/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human soluble CD40 in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits • CD Kits & Sets
 
 
ALX-850-064 Revised 23-Apr-08
CD40L, Soluble (human) (recombinant) Set
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SYNONYMS TNFSF 5, Soluble (human) (recombinant) Set
CD154, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-064-KI01   1 Set 440.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold.
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute CD40L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD40L binds to human CD40.
APPLICATION: Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Abstract; Full Text
CD40 induces apoptosis in carcinoma cells through activation of cytotoxic ligands of the tumor necrosis factor superfamily: A.G. Eliopoulos, et al.; Mol. Cell. Biol. 20, 5503 (2000) Abstract
Brain dendritic cells and macrophages/microglia in central nervous system inflammation: H.G. Fischer & G. Reichmann; J. Immunol. 166, 2717 (2001) Abstract; Full Text
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Abstract; Full Text
The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines: A. Gjorloff Wingren, et al.; J. Immunol. 168, 5582 (2002) Abstract; Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells: Y. Tanaka, et al.; J. Immunol. 170, 1291 (2003) Abstract; Full Text
IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo: A. Soruri, et al.; J. Immunol. 170, 3306 (2003) Abstract; Full Text
CD40 induces human multiple myeloma cell migration via phosphatidylinositol 3-kinase/AKT/NF-kappa B signaling: Y.T. Tai, et al.; Blood 101, 2762 (2003) Abstract
TNF receptor-associated factor 6 is an essential mediator of CD40-activated proinflammatory pathways in monocytes and macrophages: L. Mukundan, et al.; J. Immunol. 174, 1018 (2005) Abstract; Full Text
Enhanced activation of human dendritic cells by inducible CD40 and Toll-like receptor-4 ligation: N. Lapteva, et al.; Cancer Res. 67, 10528 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

CD40L (Prod. No. ALX-522-015) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-850-075 Revised 23-Apr-08
CD40L, Soluble (mouse) (recombinant) Set
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SYNONYMS CD154, Soluble (mouse) (recombinant) Set
TNFSF 5, Soluble (mouse) (recombinant) Set
gp39, Soluble (mouse) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-075-KI01   1 Set 495.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: CD40L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1’000-fold.
PURITY: ≥90% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute CD40L with 100µl sterile water and each vial of enhancer with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD40L binds to human and mouse CD40 in an ELISA assay.
APPLICATION: ELISA
Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The persistent elimination of B cells responding to blood group A carbohydrates by synthetic group A carbohydrates and B-1 cell differentiation blockade: novel concept in preventing antibody-mediated rejection in ABO-incompatible transplantation: T. Irei, et al.; Blood 110, 4567 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-850-030 Revised 17-Apr-08
CD40L, Soluble (human) ELISA Kit
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SYNONYMS CD154, Soluble (human) ELISA Kit
TNFSF 5, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-030-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.062ng/ml (range 0.16 to 10ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of soluble human CD40L (CD154) in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
HANDLING: Store controls at -20°C. Store rest of the kit at +4°C.
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-850-311 Revised 14-Apr-08
CD40L, Soluble (human) ELISA Kit (high sensitivity)
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SYNONYMS CD154, Soluble (human) ELISA Kit (high sensitivity)
TNFSF 5, Soluble (human) ELISA Kit (high sensitivity)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-311-KI01   1 Kit 682.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.005ng/ml (range 0.08 to 5ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of soluble human CD40L (CD154) in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
HANDLING: Store controls at -20°C. Store rest of the kit at +4°C.
Product Specific Literature References
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells: A. Solanilla, et al.; Blood 95, 3758 (2000) Abstract
Levels of soluble CD40 ligand (CD154) in serum are increased in human immunodeficiency virus type 1-infected patients and correlate with CD4(+) T-cell counts: N.V. Sipsas, et al.; Clin. Diagn. Lab. Immunol. 9, 558 (2002) Abstract
Increased soluble and platelet-associated CD40 ligand in essential thrombocythemia and reactive thrombocytosis: J.F. Viallard, et al.; Blood 99, 2612 (2002) Abstract
Measurement of soluble P-selectin and soluble CD40 ligand in serum and plasma: J. Thom, et al.; J. Thromb. Haemost. 2, 2067 (2004) Abstract
Soluble CD40 ligand in acute and chronic heart failure: T. Ueland, et al.; Eur. Heart. J. 26, 1101 (2005) Abstract
Increased levels of soluble CD40L in African tick bite fever: possible involvement of TLRs in the pathogenic interaction between Rickettsia africae, endothelial cells, and platelets: J.K. Damas, et al.; J. Immunol. 177, 2699 (2006) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits • CD Kits & Sets
 
 
ALX-850-053 Revised 17-Apr-08
sCD44std, Soluble (human) ELISA Kit
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY CD44 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-053-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.015ng/ml (range 0.12 to 4ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of soluble human CD44std levels in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa: K.H. Heider, et al.; Cancer Res. 53, 4197 (1993) Abstract
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44: G. Koopman, et al.; J. Exp. Med. 177, 897 (1993) Abstract
Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression: V.J. Wielenga, et al.; Cancer Res. 53, 4754 (1993) Abstract
Human mammary carcinomas express homologues of rat metastasis-associated variants of CD44: H.P. Sinn, et al.; Breast Cancer Res. Treat. 36, 307 (1995) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-850-054 Revised 24-Feb-05
sCD44var(v5), Soluble (human) ELISA Kit
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY CD44 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-054-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.22ng/ml
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative detection of soluble CD44var(v5) levels in cell culture supernatants, human serum, plasma, amniotic fluid or other body fluids. Detects all circulating CD44 isoforms comprising the sCD44var(v5) sequences.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
Differential expression of the CD44 molecule in human brain tumours: M.C. Kuppner, et al.; Int. J. Cancer 50, 572 (1992) Abstract
Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa: K.H. Heider, et al.; Cancer Res. 53, 4197 (1993) Abstract
A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps: K.H. Heider, et al.; J. Cell Biol. 120, 227 (1993) Abstract
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44: G. Koopman, et al.; J. Exp. Med. 177, 897 (1993) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-850-055 Revised 28-Apr-08
sCD44var(v6), Soluble (human) ELISA Kit
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY CD44 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-055-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.05ng/ml (range 0.32 to 10ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of soluble human CD44var(v6) levels in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa: K.H. Heider; Cancer Res. 53, 4197 (1993) Abstract
Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44: G. Koopman, et al.; J. Exp. Med. 177, 897 (1993) Abstract
Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression: V.J. Wielenga, et al.; Cancer Res. 53, 4754 (1993) Abstract
Human mammary carcinomas express homologues of rat metastasis-associated variants of CD44: H.P. Sinn, et al.; Breast Cancer Res. Treat. 36, 307 (1995) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-850-238 Revised 17-Apr-08
CD162, Soluble (human) ELISA Kit