ALEXIS Biochemicals: Z-D(OMe)E(OMe)VD(OMe)-FMK

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ALX-151-031

Revised 23-Nov-06

Caspase-3 Control Peptide
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-151-031-M001		1 mg	35.00 USD
ALX-151-031-M005		5 mg	120.00 USD

Product Specification

SEQUENCE:	Ac-Asp-Glu-Val-Asp-OH
FORMULA:	C₂₀H₃₀N₄O₁₂
MW:	518.5
PURITY:	≥97%
APPEARANCE:	Lyophilized.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Further Categories Containing This Product:

Peptides

ALX-260-016

Revised 10-Feb-05

Ac-YVAD-2,6-dimethylbenzoyloxymethylketone
SYNONYMS	Caspase-1 Inhibitor
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-016-M001		1 mg	75.00 USD
ALX-260-016-M005		5 mg	290.00 USD

Product Specification

SEQUENCE:	Ac-Tyr-Val-Ala-Asp-2,6-dimethylbenzoyloxymethylketone
FORMULA:	C₃₃H₄₂N₄O₁₀
MW:	654.7
PEPTIDE CONTENT:	78-99%
PURITY:	≥95% (HPLC)
APPEARANCE:	White powder.
SOLUBILITY:	Soluble in DMSO or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.
HANDLING:	Keep cool and dry.

Product Description

Irreversible, cell permeable inhibitor of caspase-1. Also inhibits caspase-4.

Product Specific Literature References

Inactivation of interleukin-1 beta converting enzyme by peptide (acyloxy)methyl ketones: N.A. Thornberry, et al.; Biochemistry 33, 3934 (1994) Abstract

ALX-260-019

Revised 23-Jun-06

Biotinyl-YVAD-CMK
SYNONYMS	Caspase-1 Inhibitor (Chloromethylketone)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-019-M001		1 mg	120.00 USD
ALX-260-019-M005		5 mg	480.00 USD

Product Specification

SEQUENCE:	Biotinyl-Tyr-Val-Ala-Asp-chloromethylketone
FORMULA:	C₃₂H₄₅N₆O₉SCl
MW:	725.3
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
FORMULATION:	Lyophilized.
SOLUBILITY:	Soluble in trifluoroacetic acid (50%).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.

Product Description

Irreversible, cell permeable inhibitor of caspase-1. Inhibits also caspase-4.

Product Specific Literature References

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994) Abstract

ALX-260-020

Revised 21-May-08

Z-VAD-FMK
SYNONYMS	Caspase Family Inhibitor (Fluoromethylketone)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-020-M001		1 mg	97.00 USD
ALX-260-020-M005		5 mg	380.00 USD

Product Specification

SEQUENCE:	Z-Val-Ala-DL-Asp-fluoromethylketone
FORMULA:	C₂₁H₂₈FN₃O₇
MW:	453.5
PEPTIDE CONTENT:	77-97%
PURITY:	≥92% (HPLC)
APPEARANCE:	White to yellowish powder.
SOLUBILITY:	Soluble in DMSO or acetonitrile (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C.

Product Description

Irreversible, cell permeable broad-spectrum caspase inhibitor.

Product Specific Literature References

An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett. 374, 303 (1995) Abstract
Neuroprotection by a caspase inhibitor in acute bacterial meningitis: J.S. Braun, et al.; Nat. Med. 5, 298 (1999) Abstract

ALX-260-027

Revised 03-May-07

Ac-YVAD-CHO
SYNONYMS	Caspase-1 Inhibitor (Aldehyde)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-027-M001		1 mg	42.00 USD
ALX-260-027-M005		5 mg	163.00 USD

Product Specification

SEQUENCE:	Ac-Tyr-Val-Ala-Asp-CHO
FORMULA:	C₂₃H₃₂N₄O₈
MW:	492.5
PEPTIDE CONTENT:	70-90%
PURITY:	≥96% (HPLC)
APPEARANCE:	White powder.
FORMULATION:	Lyophilized. TFA salt.
SOLUBILITY:	Soluble in DMSO or water.
RECONSTITUTION:	Add 0.2ml DMSO per 1.0mg peptide. Shake well until all the contents are dissolved. This furnishes a 10mM solution of this material.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.
HANDLING:	Keep cool and dry. Keep under inert gas.

Product Description

Potent and reversible inhibitor of caspase-1. Also inhibits caspase-4. Strongly inhibits anti-Fas induced apoptosis in L929-Fas cells.

Product Specific Literature References

K.T. Chapman; Bioorg. Med. Chem. Lett. 2, 613 (1992)
A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992) Abstract
Interleukin 1 β (IL-1 β) processing in murine macrophages requires a structurally conserved homologue of human IL-1 β converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993) Abstract
Crystal structure of the cysteine protease interleukin-1 beta- converting enzyme: a (p20/p10)2 homodimer: N.P.C. Walker, et al.; Cell 78, 343 (1994) Abstract
Structure and mechanism of interleukin-1 β converting enzyme: K.P. Wilson, et al.; Nature 370, 270 (1994) Abstract
Requirement of an ICE/CED-3 protease for Fas/APO-1-mediated apoptosis: M. Los, et al.; Nature 375, 81 (1995) Abstract
Peptide inhibitors of the ICE protease family arrest programmed cell death of motoneurons in vivo and in vitro: C.E. Milligan, et al.; Neuron 15, 385 (1995) Abstract
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis: M. Enari, et al.; Nature 380, 723 (1996) Abstract
Role of the nuclear localization sequence in fibroblast growth factor-1-stimulated mitogenic pathways: Y.Z. Lin, et al.; J. Biol. Chem. 271, 3505 (1996) Abstract; Full Text
Inhibition of human caspases by peptide-based and macromolecular inhibitors: M. Garcia-Calvo, et al.; J. Biol. Chem. 273, 32608 (1998) Abstract; Full Text

ALX-260-028

Revised 27-Mar-08

Ac-YVAD-CMK
SYNONYMS	Caspase-1 Inhibitor (Chloromethylketone)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-028-M001		1 mg	55.00 USD
ALX-260-028-M005		5 mg	220.00 USD

Product Specification

SEQUENCE:	Ac-Tyr-Val-Ala-Asp-chloromethylketone
FORMULA:	C₂₄H₃₃N₄O₈Cl
MW:	541.0
PURITY:	≥96% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO (50mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.

Product Description

Irreversible, cell permeable inhibitor of caspase-1. Also inhibits caspase-4. Inhibits Fas-mediated apoptosis.

Product Specific Literature References

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994) Abstract
Crystal structure of the cysteine protease interleukin-1 beta- converting enzyme: a (p20/p10)2 homodimer: N.P.C. Walker, et al.; Cell 78, 343 (1994) Abstract
Involvement of an ICE-like protease in Fas-mediated apoptosis: M. Enari, et al.; Nature 375, 78 (1995)
Involvement of interleukin-1 beta-converting enzyme in apoptosis of bFGF-deprived murine aortic endothelial cells: S. Kondo, et al.; FASEB J. 10, 1192 (1996) Abstract
Peptide inhibitors of the ICE protease family arrest programmed cell death of motoneurons in vivo and in vitro: C.E. Milligan, et al.; Neuron 15, 385 (1995) Abstract
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis: M. Enari, et al.; Nature 380, 723 (1996) Abstract
ICE inhibitor YVADcmk is a potent therapeutic agent against in vivo liver apoptosis: N. Rouquet, et al.; Curr. Biol. 6, 1192 (1996) Abstract
Caspase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses: G. Mathiak, et al.; Br. J. Pharmacol. 131, 383 (2000) Abstract

ALX-260-029

Revised 22-Jul-08

Z-Asp-2,6-dichlorobenzoyloxymethylketone
SYNONYMS	Caspase Family Inhibitor
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-029-M010		10 mg	80.00 USD
ALX-260-029-M050		50 mg	320.00 USD

Product Specification

FORMULA:	C₂₀H₁₇Cl₂NO₇
MW:	454.3
CAS NUMBER:	153088-73-4
PURITY:	≥98% (TLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in methanol (50mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.
HANDLING:	Keep cool and dry.

Product Description

Irreversible, cell permeable broad-spectrum caspase inhibitor.

Product Specific Literature References

P1 aspartate-based peptide alpha-((2,6-dichlorobenzoyl)oxy)methyl ketones as potent time-dependent inhibitors of interleukin-1 beta- converting enzyme: R.E. Dolle, et al.; J. Med. Chem. 37, 563 (1994) Abstract
Aspartate-based inhibitor of interleukin-1 beta-converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells: T. Mashima, et al.; BBRC 209, 907 (1995) Abstract
Involvement of mitochondria and caspase-3 in ET-18-OCH(3)-induced apoptosis of human leukemic cells: C. Gajate, et al.; Int. J. Cancer 86, 208 (2000) Abstract
Induction of apoptosis in leukemic cells by the reversible microtubule-disrupting agent 2-methoxy-5-(2',3',4'-trimethoxyphenyl)-2,4,6-cycloheptatrien-1 -one: protection by Bcl-2 and Bcl-X(L) and cell cycle arrest: C. Gajate, et al.; Cancer Res. 60, 2651 (2000) Abstract; Full Text
Inhibition of the VEGF receptor 2 combined with chronic hypoxia causes cell death-dependent pulmonary endothelial cell proliferation and severe pulmonary hypertension: L. Taraseviciene-Stewart; FASEB J. 15, 427 (2001) Abstract; Full Text
Long-term caspase inhibition ameliorates apoptosis, reduces myocardial troponin-I cleavage, protects left ventricular function, and attenuates remodeling in rats with myocardial infarction: Y. Chandrashekhar, et al.; J. Am. Coll. Cardiol. 43, 295 (2004) Abstract

ALX-260-030

Revised 05-Jun-08

Ac-DEVD-CHO
SYNONYMS	Caspase-3 Inhibitor (Aldehyde) Caspase-7 Inhibitor (Aldehyde)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-030-M001		1 mg	47.00 USD
ALX-260-030-M005		5 mg	183.00 USD

Product Specification

SEQUENCE:	Ac-Asp-Glu-Val-Asp-CHO
FORMULA:	C₂₀H₃₀N₄O₁₁
MW:	502.5
PEPTIDE CONTENT:	70-90%.
PURITY:	≥95% (HPLC)
FORMULATION:	Lyophilized.
SOLUBILITY:	Soluble in distilled water or DMSO.
RECONSTITUTION:	Add 0.2ml DMSO per 1mg peptide. Shake well until all the contents are dissolved. This furnishes a 10mM solution of this material.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C. Solutions are stable for at least 1 month when stored at -20°C in the dark and protected from moisture.
HANDLING:	Keep cool and dry. Keep under nitrogen.

Product Description

Reversible inhibitor of caspase-3 and -7.

Product Specific Literature References

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995) Abstract
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis: M. Enari, et al.; Nature 380, 723 (1996) Abstract
ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D.W. Nicholson; Nature Biotechnol. 14, 297 (1996) Abstract
CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel, et al.; J. Biol. Chem. 271, 1841 (1996) Abstract; Full Text
A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem. 272, 13432 (1997) Abstract; Full Text
Inhibition of human caspases by peptide-based and macromolecular inhibitors: M. Garcia-Calvo, et al.; J. Biol. Chem. 273, 32608 (1998) Abstract; Full Text
Inhibition of ubiquitin-proteasome pathway activates a caspase-3-like protease and induces Bcl-2 cleavage in human M-07e leukaemic cells: X.M. Zhang, et al.; Biochem. J. 340, 127 (1999) Abstract; Full Text

ALX-260-034

Revised 10-Feb-05

Biotinyl-DEVD-CHO
SYNONYMS	Caspase-3 Inhibitor (Aldehyde) Caspase-7 Inhibitor (Aldehyde)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-034-M001		1 mg	82.00 USD
ALX-260-034-M005		5 mg	323.00 USD

Product Specification

SEQUENCE:	Biotinyl-Asp-Glu-Val-Asp-CHO
FORMULA:	C₂₈H₄₂N₆O₁₂S
MW:	686.7
PEPTIDE CONTENT:	65-90%.
PURITY:	≥99% (HPLC)
APPEARANCE:	White powder.
FORMULATION:	Lyophilized.
SOLUBILITY:	Soluble in 50% acetic acid with agitation (1mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Packaged under inert gas.

Product Description

Reversible inhibitor of caspase-3 and -7. Useful for the purification or extract depletion of caspase-3 in conjunction with streptavidin conjugates.

Product Specific Literature References

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995) Abstract

ALX-260-039

Revised 05-Jan-06

Z-VAD(OMe)-FMK
SYNONYMS	Caspase Family Inhibitor (Fluoromethylketone)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-039-M001		1 mg	170.00 USD
ALX-260-039-M005		5 mg	585.00 USD

Product Specification

SEQUENCE:	Z-Val-Ala-Asp(OMe)-fluoromethylketone
FORMULA:	C₂₂H₃₀N₃O₇F
MW:	467.5
CAS NUMBER:	187389-52-2
PURITY:	≥95.0%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions should be divided into aliquots and stored at -20°C.
HANDLING:	Keep cool and dry.

Product Description

Irreversible and cell permeable broad-spectrum caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). For cell free assays use Prod. No. ALX-260-020.

Product Specific Literature References

Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes: S.C. Chow, et al.; FEBS Lett. 364, 134 (1995) Abstract
An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett. 374, 303 (1995) Abstract
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med. 184, 2445 (1996) Abstract
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J. 315, 21 (1996) Abstract; Full Text
A specific inhibitor of apoptosis decreases tissue injury after intestinal ischemia-reperfusion in mice: A. Farber, et al.; J. Vasc. Surg. 30, 752 (1999) Abstract; Full Text
Correlation of tumor necrosis factor α (TNF α) with high Caspase 3-like activity in myelodysplastic syndromes: S.D. Mundle, et al.; Cancer Lett. 140, 201 (1999) Abstract
TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk: P.F. Piguet, et al.; Lab. Invest. 79, 495 (1999) Abstract
Monocytic cell necrosis is mediated by potassium depletion and caspase-like protease: M. Warny & C.P. Kelly; Am. J. Physiol. 276, C717 (1999) Abstract; Full Text
Serine protease inhibitors suppress cytochrome c-mediated caspase-9 activation and apoptosis during hypoxia-reoxygenation: Z. Dong, et al.; Biochem. J. 347, 669 (2000) Abstract; Full Text
Apoptosi