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TNF-alpha
You are here: Product Lines > Chemokines & Cytokines > TNF & TNF Receptor Superfamily/Related Products > TNF-alpha & TNF Receptors/Related Products > TNF-alpha
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Items 14 of 14
ALX-804-253 Revised 18-May-06
Monoclonal Antibody to TNF-α (mouse) (MP6-XT22)
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SYNONYMS anti-Tumor Necrosis Factor-α (mouse) MAb (MP6-XT22)
anti-TNFSF 2 (mouse) MAb (MP6-XT22)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-253-C100   100 µg 264.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: MP6-XT22
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G affinity-purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouseTNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes mouse TNF-α.
APPLICATION: Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-252 Revised 22-Oct-07
Monoclonal Antibody to TNF-α (mouse) (MP6-XT3)
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SYNONYMS anti-Tumor Necrosis Factor-α (mouse) MAb (MP6-XT3)
anti-TNFSF 2 (mouse) (MP6-XT3)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-252-C100   100 µg 264.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: MP6-XT3
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes mouse TNF-α.
APPLICATION: ELISA
Western Blot (1-5µg/ml)
Functional Application (Neutralization)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-199 Revised 18-Sep-07
Monoclonal Antibody to TNF-α (human) (TNF-D)
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SYNONYMS anti-Tumor Necrosis Factor-α (human) MAb (TNF-D)
anti-TNFSF 2 (human) MAb (TNF-D)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-199-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: TNF-D
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Recombinant human TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes human TNF-α. Does not cross-react with mouse TNF-α or human or mouse TNF-β.
APPLICATION: ELISA: Use as capture or detection antibody in sandwich ELISA.
Flow Cytometry: Suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with  monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes.
Functional Application: Selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening aliquot contents and freeze at -20°C.
Product Specific Literature References
Monoclonal antibodies to human tumor necrosis factors alpha and beta: application for affinity purification, immunoassays, and as structural probes: T.S. Bringman & B.B. Aggarwal; Hybridoma 6, 489 (1987) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-371 Revised 01-Jun-06
Monoclonal Antibody to TNF-α (mouse) (1F3F3D4 IGH 156)
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SYNONYMS anti-TNFSF 2 (mouse) MAb (1F3F3D4IGH 156)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-371-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 1F3F3D4 IGH 156
ISOTYPE: Rat IgM
CONCENTRATION: 0.1mg/ml
PURITY: >95% (SDS-PAGE).
FORMULATION: Liquid. Antibody in PBS.
IMMUNOGEN: Recombinant human TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes mouse TNF-α.
APPLICATION: ELISA
Functional Application (neutralizing): 25ng/ml neutralizes the bioactivity of 40pg/ml recombinant mouse TNF-α by >95%.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-335 Revised 29-Aug-06
Polyclonal Antibody to TNF-α
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SYNONYMS anti-Tumor Necrosis Factor-α PAb
anti-TNFSF 2 PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-335-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY: ≥99% (SDS-PAGE).
FORMULATION: Liquid. In PBS.
ENDOTOXIN CONTENT: ≤0.0005EU/µg protein.
IMMUNOGEN: Recombinant rat TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes human, mouse and rat TNF-α.
APPLICATION: ELISA
Western Blot: 10ng recombinant rat TNF-α by 40-60µg/ml Prod. No. ALX-210-335.
Functional Application: Neutralizes cytotoxic activity of recombinant rat TNF-α (Neutralizing activity: >2x105U/mg. One unit is defined as the amount of antibody sufficient for neutralizing the cytotoxic activity of 1 unit recombinant rat TNF-α).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-522-087 Revised 07-Feb-06
Fc (human):TNF-α (human), Soluble (recombinant)
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SYNONYMS Fc (human):Tumor Necrosis Factor-α (human), Soluble (recombinant)
Fc (human):TNFSF2 (human), Soluble (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-087-C010   10 µg 395.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~48kDa
SOURCE/HOST: Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test; BioWhittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY: Induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer).
APPLICATION: Flow Cytometry (1:100)
Immunoprecipitation (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Life and death decisions: secondary complexes and lipid rafts in TNF receptor family signal transduction: J.R. Muppidi, et al.; Immunity 21, 461 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-520-002 Revised 05-Jan-07
TNF-α (human) (recombinant) (cell culture grade)
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SYNONYMS Tumor Necrosis Factor-α (human) (recombinant) (cell culture grade)
TNFSF 2 (human) (recombinant) (cell culture grade)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-520-002-C010   10 µg 183.00 USD Add To Cart
ALX-520-002-C050   50 µg 550.00 USD Add To Cart
Product Specification
MW: ~17kDa.
CAS NUMBER: 94948-59-1
MERCK INDEX: 14: 9812
SOURCE/HOST: Produced in yeast.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized. Contains 50µg D-mannitol/µg TNF-α.
ENDOTOXIN CONTENT: <1EU/µg protein
RECONSTITUTION: A quick spin is recommended followed by reconstitution in distilled, sterile water to a concentration of 0.1-1mg/ml. For further dilution, add a carrier protein to avoid loss of biological activity.
BIOLOGICAL ACTIVITY: Induces cytolysis of mouse L929 cells in the presence of 1µg/ml Actinomycin D (Prod. No. ALX-380-009) (ED50~0.02-0.08ng/ml).
SPECIFIC ACTIVITY: ≥2x107U/mg protein. Determined by standard cell cytotoxicity assay (N. Matthes & M.L. Neale; Lymphokines and Interferons, A Practical Approach; M.J. Clemens et al., eds.; IRL Press, p. 221).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Lyophilized protein is stable at room temperature for a few weeks.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-008 Revised 18-Sep-07
TNF-α, Soluble (human) (recombinant)
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SYNONYMS Tumor Necrosis Factor-α, Soluble (human) (recombinant)
TNFSF 2, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-008-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~19kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-060), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY: Exerts its biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells).
SPECIFIC ACTIVITY: ED50: 0.1ng/ml (WEHI 164 cells)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003) Abstract; Full Text
Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes: O. Micheau & J. Tschopp; Cell 114, 181 (2003) Abstract
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003) Abstract; Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) Abstract
The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed: L. Riol-Blanco, et al.; J. Immunol. 174, 4070 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01375: TNF-α (human) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-850-060 Revised 23-Apr-08
TNF-α, Soluble (human) (recombinant) Set
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SYNONYMS Tumor Necrosis Factor-α, Soluble (human) (recombinant) Set
TNFSF 2, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-060-KI01   1 Set 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in E. coli. The extracellular domain of human TNF-α (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 50µg of TNF-α, Soluble (human) (recombinant) (Prod. No. ALX-522-008) and 2x50µg Enhancer for Ligands (Prod. No. ALX-804-034).
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute the contents of each tube with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: TNF-α binds to human and mouse TNF-R1 and TNF-R2. In the presence of cross-linking enhancer, TNF-α preferentially binds to human and mouse TNF-R2.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
NF-kappaB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell Biol. 21, 5299 (2001) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Receptor/Ligand + Enhancer Set
 
 
ALX-522-009 Revised 22-Aug-07
TNF-α, Soluble (mouse) (recombinant)
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SYNONYMS Tumor Necrosis Factor-α, Soluble (mouse) (recombinant)
TNFSF 2, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-009-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
MW: ~20kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY: Exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells). 
SPECIFIC ACTIVITY: ED50=0.02ng/ml (WEHI 164 cells)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P06804: TNF-α (mouse) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994) Abstract
Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994) Abstract
The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-850-061 Revised 23-Apr-08
TNF-α, Soluble (mouse) (recombinant) Set
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SYNONYMS Tumor Necrosis Factor-α, Soluble (human) (recombinant) Set
TNFSF 2, Soluble (human) (